BMG Labtech FLUOstar OPTIMA Operating Manual Download | Manualshive

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Operating Manual

Revision I

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Summary of Contents for FLUOstar OPTIMA

Page 1: ...Operating Manual Revision I...

Page 2: ...3930 94500 Champigny s Marne Omiya ku 330 0844 Phone 61 3 5973 4744 Phone 33 1 48 86 20 20 Saitama City Japan Fax 61 3 5973 4711 Fax 33 1 48 86 47 07 Phone 81 48 647 7217 australia bmglabtech com fra...

Page 3: ...ure in the above mentioned modes and additionally in fluorescence polarization mode and simultaneous dual emission The LUMIstar OPTIMA is a luminescence microplate reader that can be upgraded to inclu...

Page 4: ...12 4 2 2 FLUORESCENCE INTENSITY OPTICS 13 4 2 3 ABSORBANCE OPTICS 13 4 2 4 LUMINESCENCE OPTICS 14 4 2 5 FLUORESCENCE POLARIZATION OPTICS 14 4 2 6 DUAL EMISSION OPTICS 15 4 2 7 HIGH DENSITY OPTICS 15 4...

Page 5: ...filter positions Gain control Software selectable gain Automatic gain adjustment Plate Carrier Auto lock microplate carrier All microplate formats up to 1536 well in all detection modes Microplates sh...

Page 6: ...10 OD for 0 2 OD range 0 030 OD for 2 3 OD range Computer interface RS232 38400 baud binary communication protocol Power requirements 100 115 230 V 50 60 Hz Consumption max 205 VA Fuses T2 5A 250V for...

Page 7: ...ng and positioning the reader make sure to unlock the transport pin section 2 1 Transport Pin before any power connection section 2 2 Power and Communication Connections 2 1 Transport Pin When the ins...

Page 8: ...ment s power cable and turning the power switch on make sure the transport pin is unlocked section 2 1 Power Connection First check that the power switch on the back of the instrument is in the Off po...

Page 9: ...A flashing green light means the instrument is busy e g performing a measurement plate in out priming etc A faster flashing 5 flashes per second means an error has occurred Back View On Off Power cabl...

Page 10: ...8 FLUOstar OPTIMA POLARstar OPTIMA top view of reagent box with combi optic and 2 reagent injectors Top View Reagent Box LUMIstar OPTIMA Transport pin Figure 9 LUMIstar OPTIMA top view of reagent box...

Page 11: ...ion and Changing of Optics The readers are equipped with quick fix mountings for easy exchange of optics and easy to position spacers for more about spacers see 4 5 No tools are required for changing...

Page 12: ...ging of Optics For fluorescence measurements excitation enters through the yellow marked light guide and emission is measured through the blue marked light guide For absorbance measurements The grey r...

Page 13: ...tics Regarding reagent injection be careful when you position the needles in the measurement head see figure 15 to avoid damage to the reagent needles as well as to the optic The optional reagent inje...

Page 14: ...Luminescence optics Regarding reagent injection be careful when you position the needles in the measurement head see figure 18 to avoid damage to the reagent needles as well as to the optic The option...

Page 15: ...ptics look like the polarization optics but are not capable of polarization The optic has to be installed in the same way as the polarization optic figure 20 4 2 7 High Density Optics The high density...

Page 16: ...Well Injection Head A special injection head is required in order to inject into 384 well plates The injection head is installed similarly to an optic see section 4 2 Installation and Changing of Opti...

Page 17: ...installation of the software the filter must be typed in the filter table figure 25 In the Control software the filter table can be reached by choosing Setup Filter see the software manual for additio...

Page 18: ...eful not to touch any filters and turn the nut counter clockwise The filter wheel can be pulled straight out of the housing The filter positions are numbered 1 to 8 When installing new filters note th...

Page 19: ...Fluorescence Polarization Filters The excitation filters can go in any available position in the excitation wheel The emission wheel has two filters of the same wavelength for each channel They shoul...

Page 20: ...ier see figure 31 spacers needs to be installed under the measurement head see figure 32 There should be enough spacers so that the height of the left side of the plate carrier is slightly higher than...

Page 21: ...nge barrel Take off the syringe barrel and rinse it with distilled water It may be useful to use the wire syringe cleaners can be found in the service box to scrape particles off the walls In order to...

Page 22: ...disinfecting the instrument use the following solutions Alcohol 70 Authorized personnel wearing disposable gloves and protective clothing should only perform the disinfection procedure The location sh...

Page 23: ...disinfected according to the instructions of the operating manual of instrument Name ________________________________________________ Company ________________________________________________ ________...

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