Fluorescence Testing
For models with fluorescence capability, BioTek provides two options for testing the
fluorescence system. One uses a solid state Fluorescence Test Plate (package BTI#
1400006*; contact BioTek Customer Care regarding availability). The other uses liquid
plates, the materials for which are available for purchase from BioTek (see
on page
).
*Fluorescence Test Plate BTI# 7092092 cannot be used for these tests.
BioTek Fluorescence Test Plate
The Fluorescence Test Plate simplifies the process for conducting fluorescence intensity,
fluorescence polarization, and time-resolved fluorescence qualification tests on the
Synergy H1. The test plate is solid and therefore immune to the pipetting errors,
evaporation issues, and costs experienced with conventional Liquid Tests.
The test plate package includes Gen5 protocols* designed specifically for use with the test
plate. The protocols include embedded Microsoft Excel spreadsheets to automatically
calculate results and determine pass/fail. The protocols and their spreadsheets were fully
validated in accordance with BioTek Instruments' Product Validation policies and
procedures.
*Gen5 version 2.06 or higher is required.
The package also contains a User Guide that describes the test methods, helps you get
started with using the plate, and provides important information for cleaning and
maintaining the test plate. The guide also provides troubleshooting tips and information on
the annual recalibration program.
Results Analysis
Refer to the
Fluorescence Test Plate User Guide
for descriptions of the data reduction
calculations for each test. The tests must meet the following criteria to pass:
Corners Test
%CV < 3.0
Sensitivity Tests:
Filters, Top, SF
Detection Limit <= 10 pM
Monochromators, Top, SF
Detection Limit <= 20 pM
Monochromators, Bottom, SF
Detection Limit <= 20 pM
Filters, Top, MUB
Detection Limit <= 160 pg/mL
Monochromators, Top, MUB
Detection Limit <= 160 pg/mL
Monochromators, Bottom, MUB
Detection Limit <= 160 pg/mL
Linearity Test
R
2
>= 0.9500 confirms that the system is linear
Time-Resolved Fluorescence (TRF) Test
Detection Limit <= 250 fM
Fluorescence Polarization (FP) Test
Mean PHPR > 340 mP, STD PLPR < 5 mP
Synergy H1 Operator's Manual
Fluorescence Testing | 87
Summary of Contents for Synergy H1
Page 1: ...Hybrid Multi Mode Microplate Reader Synergy H1 TM Operator s Manual ...
Page 2: ......
Page 26: ...BioTek Instruments Inc xxiv Preface ...
Page 54: ...BioTek Instruments Inc 28 Chapter 2 Installation ...
Page 68: ...BioTek Instruments Inc 42 Chapter 3 Getting Started ...
Page 132: ...BioTek Instruments Inc 106 Chapter 7 Instrument Qualification Process ...
