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ACD HybEZ

™

II Hybridization System User Manual

321710-USM Date 05112017

2

For Research Use Only. Not for diagnostic use.

RNAscope

®

, BaseScope

™

, HybEZ

™

, and EZ-Batch

™

are trademarks or registered trademarks of Advanced Cell

Diagnostics, Inc. All other trademarks belong to their respective owners.

When describing a procedure for publication using these product, please refer to them as the ACD

HybEZ

™

II

Hybridization System (Cat. No. 321710 and Cat. No. 321720).

Advanced Cell Diagnostics, Inc. reserves the right to change its products and services at any time to incorporate
technological developments. This manual is subject to change without notice.

Although this manual has been prepared with every precaution to ensure accuracy, Advanced Cell Diagnostics,
Inc. assumes no liability for any errors, omissions, or for any damages resulting from the use of this information.

© 2017. Advanced Cell Diagnostics, Inc. All rights reserved.

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Summary of Contents for ACD EZ-Batch

Page 1: ...Copyright C COSMOBIO CO LTD HybEZTM Ⅱ Hybridization System 簡易マニュアルかならず英語版マニュアルもご参照ください ...

Page 2: ...Copyright C COSMOBIO CO LTD 構成品 2 オーブン本体写真電源コードユーザーマニュアル HybEZ 浸潤トレイ品番310012 写真 EZ Batch スライドホルダー品番310017 写真 EZ Batch 洗浄トレイ品番310019 写真浸潤ペーパー2枚品番310025の一部 ...

Page 3: ...Copyright C COSMOBIO CO LTD ディスプレイ画面について HybEZ Ⅱ Ovenの電源を入れると RNAscopeモードになっていますディスプレイの1列目に RNAscope と表示されます 2列目の左に設定温度 40 が右に現在の温度点滅が表示されますオーブン内の温度が設定温度 2 5 を超えた場合アラームが鳴ります ...

Page 4: ...セイ用に温度を40 に保つモードです Bake モードスライドのベイキング用のモードです開始すると温度が上昇し 60 に達した時点で60分のタイマーがスタートし自動的に終了します Hold T モード室温 10 75 内の任意の温度に設定するモードです P Run モードお好みのプログラムを10個までプリセット可能です 1つのプログラム中に 3段階の温度時間を設定できますモードの切り替えについて 1 Modeボタンを押し 4つのモードを表示する 2 or ボタンを押しモードを選択する選択されたモードは点滅します 3 Enterボタンで決定する ...

Page 5: ...Copyright C COSMOBIO CO LTD 湿潤トレイの準備 5 1 浸潤ペーパーを1枚浸潤トレイの中央に載せます 2 50mL程度の蒸留水で湿潤ペーパーを湿らせて使用します ...

Page 6: ...Copyright C COSMOBIO CO LTD EZ Batch スライドホルダーの使用方法スライドの外端の回し留め金を外しますスライドのラベルを外側し内側をホルダー中央の溝に入れるようにセットしますスライドの外端の留め金を回しスライドを固定します最大20スライドまで同時に洗浄操作が可能になります ...

Page 7: ...イドホルダーへセットします 2 EZ Batch スライドホルダーを浸潤トレイの上へ置きます湿潤ペーパーは入れないトレイの蓋もしない 3 トレイをオーブンへ入れます 4 Modeボタンを押します 5 or ボタンでBakeモードを選択します 6 Enterボタンを押します 7 Baking Slide と現在の温度が表示されますオーブン内の温度が60 に達したら60分からカウントダウンが始まります 8 カウントダウンが終了したらお知らせの発信音がします 9 オーブン内の温度が室温まで冷却されディスプレイがモード選択画面に戻ります ...

Page 8: ...を押します 2 or ボタンを押してHold Tモードを選択します 3 Enterボタンを押します 4 ディスプレイの1列目に Hold Temp と表示されます 2列目の左に設定温度が右に現在の温度が表示されます 5 or ボタンを押し任意の温度を設定します 6 Enterボタンを押して任意の温度を決定します 7 オーブン内の温度が設定温度へ達した後にサンプルのインキュベートがはじまります 8 Modeボタンを押すといつでもHold Tモードを終了できますオーブン内の温度が設定温度 2 5 を超えた場合アラームが鳴ります ...

Page 9: ...表示されます 2列目の左に Run 右に Setup と表示されます 4 or ボタンを押し Setupを選択します点滅しています 5 Enterボタンを押します 6 Program n と表示されているので or ボタンを押して番号を設定し Enterボタンを押します 7 Temp 1 xx x と表示されているので or ボタンを押して任意の温度を設定してEnterボタンを押します 8 Time 1 mm ss と表示されているので or ボタンを押して任意の温度を設定してEnterボタンを押します 9 7 および8 の操作を繰り返してステージ2 3の温度と時間も設定しますプログラムの設定 P Runモード ...

Page 10: ...med Run と表示されます 2列目の左に Run 右に Setup と表示されます 3 or ボタンを押し Runを選択します点滅しています 4 Enterボタンを押します 5 ディスプレイの1列目に Run 2列目に Program n と表示されます 6 or ボタンを押して0 9のプログラムを選択します 7 Enterボタンを押して使用するプログラムを実行します 8 プログラムが終了したらオーブン内の温度は室内の温度に戻りディスプレイも戻ります 9 プログラム実行中にModeボタンを押すといつでも中止することができますプログラムの実行 P Runモード ...

Page 11: ...For Research Use Only Not for diagnostic use 321710 USM Date 05112017 ...

Page 12: ...ease refer to them as the ACD HybEZ II Hybridization System Cat No 321710 and Cat No 321720 Advanced Cell Diagnostics Inc reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without notice Although this manual has been prepared with every precaution to ensure accuracy Advanced Cell Diagnostics Inc assumes no ...

Page 13: ...ybEZ II Hybridization System User Manual 321710 USM Date 05112017 3 Background 6 Intended Use 6 EZ Batch Slide Holder 7 EZ Batch Wash Tray 7 General 8 Technical Specifications 8 To enter a different mode 17 ...

Page 14: ...ACD HybEZ II Hybridization System User Manual 321710 USM Date 05112017 4 Limited product warranty 19 Returns 19 ...

Page 15: ...r l appareil Always observe the following safety precautions Read this entire manual before using the slide hybridization oven Use only approved accessories Do not modify system or components Any alterations or modifications to your incubator may be dangerous and will void the warranty Do not position the equipment so that it is difficult to operate the disconnecting device Use only as specified b...

Page 16: ...oss of reagent due to evaporation These processes often require separate slide handling devices When many slides are processed in a batch transferring slides repeatedly between two different devices becomes tedious labor intensive and error prone The ACD HybEZ II Hybridization System is a new generation evaporation controlled incubator designed to conduct the hybridization and incubation steps in ...

Page 17: ...0 standard microscope slides in secure lock down positions arranged in two parallel rows Lock down is achieved by two lockable swing clamps one per row along both sides of the slide holder Clamp locking mechanisms are located at the slots found at one end of each clamp The following is a diagram of the EZ Batch Wash Tray Cat No 310019 IMPORTANT The ACD EZ Batch Slide Processing System is not compa...

Page 18: ... humidity of 80 non condensing Installation Category II transient voltages Pollution Degree 2 in accordance with IEC 664 Suitable for operation at altitudes of up to 6500 feet Ambient 10 C to 75 C 20 C to 75 C 0 5 C 0 5 C at 40 C 0 1 C Yes in cases of temperature deviation step complete or program complete Yes 115 V 10 50 60 Hz 230 V 10 50 60 Hz 155 W Ambient to 40 C within 20 minutes 11 00 x 15 9...

Page 19: ...wer Line Cord HybEZ II Hybridization System User Manual HybEZ Humidity Control Tray includes a tray with gasket installed and lid HybEZ Humidifying Paper EZ Batch Slide Holder EZ Batch Wash Tray May be sent in a different package To install the system 1 Place the HybEZ II Oven on a flat and stable surface preferably away from drafts 2 Fit the power line cord into the IEC power socket on the rear o...

Page 20: ...me the unit is turned on If you are using or have used the HybEZ II Oven for functions other than running RNAscope assays return to the RNAscope mode by pressing the Mode then Enter buttons on the front panel see Appendix B Other Functions on page 17 for details Note The oven will produce an audible alarm if the actual chamber temperature has deviated more than 2 5 C of the set 40 C temperature 1 ...

Page 21: ...eezing the slotted portion of each clamp and rotating it as shown in the following figure 2 Insert up to 10 slides one at a time into one row of the holder The non label end of each slide should be aligned toward the center of the holder and inserted under the fixed clamp as shown Rotate the rest of the slide down into the holder ...

Page 22: ...n the clamp 4 Repeat steps 2 and 3 with the other row of the holder Note Once all slides are locked into the holder you can handle them as an integrated unit 1 Use your standard protocol to add reagents to each slide Note To prevent samples from drying out when applying reagents quickly add one drop of reagent to each sample Then add enough reagent volume to completely cover the samples 2 Place th...

Page 23: ...emoving the tray due to potentially high temperatures The use of gloves may be necessary To remove the HybEZ Humidity Control Tray from the HybEZ II Oven 1 Open the main door of the oven 2 Slide the tray out 3 Close the oven door 1 Pour 300 mL 1X Wash Buffer into the EZ Batch Wash Tray 2 Place the EZ Batch Slide Holder into the Wash Tray Make sure all slides are submerged in solution 3 Incubate th...

Page 24: ... Unit not turned on b Unit not plugged into power supply c Fuses blown d Power supply failure a Push the Power Switch to turn on the unit b Plug in and turn on c Replace fuses see Appendix A Maintenance d Check that other electrical appliances on the same circuit are working a Ambient temperature is higher than set temperature b Temperature control circuit fault a Check set temperature b Have unit...

Page 25: ...ACD HybEZ II Hybridization System User Manual 321710 USM Date 05112017 15 b Lid bent c Insufficient buffer placed in the bottom of tray b Replace the lid c Place more buffer in the bottom of tray ...

Page 26: ... cleaning or decontamination method except those recommended by the manufacturer check that the proposed method will not damage equipment Two supply fuses are located in the fuse drawer just above the Power Switch To replace the fuses 1 Disconnect the unit from the power supply 2 Remove the plug from the socket in the back of the unit 3 Open the door of the power entry module to expose the fuse dr...

Page 27: ...rograms once Each program comprises three different temperature stages at user defined temperatures and durations Press the Mode button on the front panel to display the four modes on the screen Use the or buttons to select the desired mode The text of the selected mode will flash on the screen Press the Enter button to enter the selected mode 1 Load tissue slides into the EZ Batch Slide Holder 2 ...

Page 28: ...uttons to select Setup The selected text flashes 5 Press the Enter button 6 The screen displays the target ID number of the program to be set as Program n Use the or buttons to adjust to the desired ID number and then press Enter 7 The screen displays the first target temperature as Temp 1 xx x Use the or buttons to adjust to the desired target temperature and then press Enter 8 The screen display...

Page 29: ...support acdbio com Advanced Cell Diagnostics Inc and or its affiliate s warrant their products as set forth in the ADC General Terms and Conditions of Sale found on the ADC website at http www acdbio com store terms When used in laboratory conditions and according to this User Manual Advanced Cell Diagnostics ACD warrants this product to be free of defective material and workmanship for a period o...

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