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ExiPrep

TM

16 Plus

Fully Automated Nucleic Acid Extraction System

 

 

-35- 

 

 

7.  Request service from your dealer. 

The Syringe Block inserted the 

tips as expected but does not 

move. 

1.  Verify that the front door is completely closed. 

2.  Visually inspect the Syringe Block to verify the absence of foreign 

materials or accessories that may interfere with normal 

movement. 

3.  Verify that the Buffer Cartridges are installed correctly. 

4.  Check to see if the ‘Stop’ or ‘Pause’ buttons are selected. 

5.  Request service from your dealer. 

The instrument stops during 

operation. 

1.  Verify that the power source is connected. 

2.  Verify that the power switch is pressed (ON position). 

3.  Check the LCD screen to verify that the blue progress bar 

(bottom portion; indicates delays) is moving. 

4.  Check to see if the ‘Stop’ or ‘Pause’ buttons are selected. 

5.  Request service from your dealer. 

The instrument is malfunctioning. 

1.  Check to see if the correct protocol was selected. 

2.  Inspect the instrument for foreign materials or accessories 

impeding normal movement of the Syringe Block (which causes 

motor malfunction). 

3.  Repeat the same protocol and verify normal operation. 

4.  Request service from your dealer. 

The instrument operates normally 

but does not elute. 

1.  Verify that the Elution Tube Rack and Elution Tubes are installed 

correctly. 

2.  Check to see if the Tips are completely inserted. 

3.  Inspect the Tips for clogging. 

4.  Verify that the Buffer Cartridges contains sample. 

5.  Inspect the Syringe Block for leakage. 

6.  Request service from your dealer. 

The Syringe Block is leaking. 

1.  Stop using the malfunctioning well and request service from your 

dealer. 

Liquid is dripping inside the 

instrument. 

1.  Make sure the Rack and Contamination Shield are installed 

correctly. 

2.  Inspect the Syringe Block for leakage. 

3.  Request service from your dealer. 

The heater does not work.  

1.  Make sure the Rack and Contamination Shield are installed 

correctly. 

2.  Inspect the Base Plate for signs of leakage. 

3.  Request service from your dealer. 

Summary of Contents for A-5030

Page 1: ...User Manual Ver 1 1 ExiPrep 16 Plus Fully Automated Nucleic Acid Extraction System...

Page 2: ...7 V DNA RNA Extraction 9 1 Contamination shield installation 9 2 Sample Preparation 10 3 RUN 12 VI ExiPrep 16 Plus Setup 15 1 Main Menu 15 2 Registering a New User 19 3 Viewing Run History 20 4 Managi...

Page 3: ...rmation about ExiPrepTM 16 Plus You can download up to date product information and new protocols General information ExiPrep 16 Plus is a trademark of Bioneer Corporation The information contained in...

Page 4: ...instrument is stopped either from operator error including improper accessory insertion or manually halting the instrument during normal operation you must re initialize the instrument before pulling...

Page 5: ...act results 3 Operate the instrument within the recommended humidity range 20 80 no condensation Humid conditions may cause corrosion or malfunction 4 Do not place any objects next to or behind the in...

Page 6: ...pulled out while the Heating Block is not in its normal initialized position the interference in movement can cause Heating Block and other internal component damage and lead to instrument malfunction...

Page 7: ...nents and Specifications 1 System Components Part Name Cat No Qty Check ExiPrep 16 Plus A 5030 1 ea User s manual 1 ea Waste tray 1 ea Elution tube rack 1 ea Disposable tip rack 1 ea Setup tray 1 ea C...

Page 8: ...mm 320 W x 487 H x 535 D Weight 22 kg Operating temperature 15 30 C Operating humidity 20 80 no condensation Operating system Stand alone Electrical Voltage Frequency 100 240 V 50 60 Hz Network suppor...

Page 9: ...door and remove the tie wrap from the belt Make sure that all components are included Refer to the list of components on page 5 1 Open the door and remove the Screw bolt A from the rail 2 Remove the P...

Page 10: ...n 7 Press the power button on the LCD screen to initialize the instrument A progress bar on the lower portion of the LCD touchscreen will indicate initialization progress 8 Open the door and remove th...

Page 11: ...nation shield CAUTION In order to use contamination shield this must be installed on the Base plate accessory 2 Place the contamination shield on the lower right side of the Syringe block Contaminatio...

Page 12: ...the DNA RNA extraction kits sold separately 3 Make sure you have purchased the correct kit for the sample type and target nucleic acid type you desire Insert the desired number of Elution tubes E int...

Page 13: ...aling the film of Buffer Cartridge using the 6 Hole punch tool in the same pattern as Buffer Cartridge 7 Load samples into the Sample Loading Well of Buffer Cartridge 8 Preparation for DNA RNA extract...

Page 14: ...id and sample source type 3 Verify the Prep Type and Sample SRC of the three digit code you have entered 4 Press the Enter button to access the elution volume selection menu 5 Select the elution volum...

Page 15: ...ake sure the Buffer Cartridge fits snugly and exactly into its place Misplacement of the Buffer Cartridge may result in instrument break down and malfunction 8c Place the Elution tube rack on the Base...

Page 16: ...the Base Plate and remove the Elution tubes Buffer Cartridges and all racks from the Base Plate After removing all accessories push the Base Plate back in completely and close the door 12 You are giv...

Page 17: ...s UV lamp ON OFF selection menu Sterilize the internal cavity with the built in UV lamp Press the UV lamp button to begin sterilization The sterilization runs for 5 minutes To cancel press the UV lamp...

Page 18: ...SETUP MENU screen PREP SETUP screen Selecting Start from the Main Menu will bring up the PREP SETUP screen where you can enter the three digit code for the extraction and sample source type Refer to t...

Page 19: ...ress the UV lamp icon to initiate the UV sterilization process The icon will turn yellow as UV sterilization proceeds The sterilization runs for 5 minutes The progress can be tracked through the progr...

Page 20: ...ing features such as UV sterilization and system preferences Details on system configuration can be found in page 23 History Enabled by selecting the user login option Allows you to audit up to 99 mos...

Page 21: ...ess the Setup button to access the SETUP menu 2 Press the User button to access the User Registration menu 3 Enter a 6 digit user ID using the keypad on the LCD touch screen and press Enter to save th...

Page 22: ...ory 1 Press the Setup button to access the SETUP menu 2 Press the History button to view the instrument run history 3 The run history contains the following parameters No Recent runs have a lower numb...

Page 23: ...button to access the SETUP menu 2 Press the Config button to access the System Setup menu 3 Press the User button to enable login mode If the login mode is enabled a popup prompt User Mode ON appears...

Page 24: ...ter every DNA RNA extraction run 1 Press the Setup button to access the SETUP menu 2 Press the Config button to access the System Setup menu 3 Press the UV lamp button to enable automatic UV steriliza...

Page 25: ...Screen Calibrates the screen position Calibrate the screen position relative to touching Press and hold the circle at the upper left corner with a blunt tool for 2 seconds Press and hold the circle a...

Page 26: ...LCD touchscreen 3 Press the Enter button 4 The Admin Menu screen includes an option to delete user IDs or change the administrator ID Select User list delete 1 to delete unused IDs Select Administrat...

Page 27: ...r ID change menu Enter a new 6 digit administrator ID using the keypad in the middle of LCD touch screen and press Enter button to save Press the ok button to save new administrator ID after verifying...

Page 28: ...ing to the following instructions The default IP address for ExiPrepTM 16 Plus is 10 10 104 88 1 Execute the program 2 Click the IP Select button on the lower left corner 3 A new window IP Machine Sel...

Page 29: ...e right side Wait until the progress bar is full If the connection stops progressing restart the update process from step 1 9 Once the connection process is done Memory Check starts window will pop up...

Page 30: ...ick the connection and select Properties iii Select Internet Protocol TCP IP iv Select Use the following IP address v Enter the IP address subnet mask Default gateway and DNS server address informatio...

Page 31: ...m 1 14 Genomic DNA Buffy coat 2 14 Total RNA Buffy coat 1 15 Genomic DNA Sputum 2 15 Total RNA Sputum 1 16 Genomic DNA BAL 2 16 Total RNA BAL 1 17 Genomic DNA Saliva 2 17 Total RNA Saliva 1 18 Genomic...

Page 32: ...DNA Serum 3 14 mRNA Buffy coat 4 14 viral DNA Buffy coat 3 15 mRNA Sputum 4 15 viral DNA Sputum 3 16 mRNA BAL 4 16 viral DNA BAL 3 17 mRNA Saliva 4 17 viral DNA Saliva 3 18 mRNA Swab 4 18 viral DNA Sw...

Page 33: ...oat 6 14 viral DNA RNA Buffy coat 5 15 viral RNA Sputum 6 15 viral DNA RNA Sputum 5 16 viral RNA BAL 6 16 viral DNA RNA BAL 5 17 viral RNA Saliva 6 17 viral DNA RNA Saliva 5 18 viral RNA Swab 6 18 vir...

Page 34: ...tem 32 continue No Target Sample source No Target Sample source 7 01 Plasmid DNA endA strain 9 01 Protein His Tag 7 02 Plasmid DNA endA strain No Target Sample source 8 21 Fragment DNA Gel slice 8 22...

Page 35: ...from your dealer Power turns on but the LCD screen is black 1 Since it is most likely an instrument issue request service from your dealer Pressing the RUN button fails to start instrument operation 1...

Page 36: ...the end of the Base Plate Slide Rail 4 Request service from your dealer The Base Plate does not slide out completely 1 Visually inspect inside the instrument for accessories and or foreign materials t...

Page 37: ...to see if the correct protocol was selected 2 Inspect the instrument for foreign materials or accessories impeding normal movement of the Syringe Block which causes motor malfunction 3 Repeat the same...

Page 38: ...top usage of the instrument 2 Request service from your dealer The UV Lamp does not work 1 Verify that the front door is completely closed 2 Request service from your dealer Protocol dose not update 1...

Page 39: ...s and broken plastic on ports b to damage caused by accident abuse misuse flood fire earthquake or other external causes c to a product or part that has been modified in any way without written permis...

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