Biohit 728010, Instruction Manual

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6 6
The Safe-Cone The Safe-Cone
fi fi
lters are available as Standard or Plus versions. It lters are available as Standard or Plus versions. It
is recommended to use the Standard is recommended to use the Standard fi fi lter for general applications lter for general applications
and the Plus and the Plus fi fi lter for more demanding applications such as cell lter for more demanding applications such as cell
culture, bacterial and virological work and molecular biology. culture, bacterial and virological work and molecular biology.
Filters need to be changed regularly. The interval for filter Filters need to be changed regularly. The interval for filter
changing is application dependant but the recommendation is to changing is application dependant but the recommendation is to
change the change the fi fi lter daily (after 50 - 250 lter daily (after 50 - 250
pipetting cycles) and always in case pipetting cycles) and always in case
of over-aspiration. (See Chapter 2 of over-aspiration. (See Chapter 2
for ordering information.) The Biohit for ordering information.) The Biohit
Filter Foreceps should be used to Filter Foreceps should be used to
avoid touching the soiled filters avoid touching the soiled filters
by hand (Fig.5). See Chapter 2 for by hand (Fig.5). See Chapter 2 for
ordering information. Make sure that ordering information. Make sure that
the the fi fi lter is disposed into a suitable lter is disposed into a suitable
waste container. Clean the tip cone waste container. Clean the tip cone
if needed and put the new if needed and put the new
fi fi
lter in lter in
place. place.
8. 8. PIPETTING PIPETTING TECHNIQUES TECHNIQUES
T T wo basic pipetting techniques, for wo basic pipetting techniques, for ward and reverse pipetting, a ward and reverse pipetting, a re re
associated with Proline associated with Proline
® ®
Plus pipettes. Plus pipettes. Forward pipetting Forward pipetting is the is the
most common used pipetting technique. The technique employs most common used pipetting technique. The technique employs
the blow-out function ensuring complete delivery of the liquid. the blow-out function ensuring complete delivery of the liquid.
Reverse pipetting Reverse pipetting is recommended for highly viscous, biological is recommended for highly viscous, biological
or foaming liquids, or very small volumes of liquid. A selected or foaming liquids, or very small volumes of liquid. A selected
volume plus an excess is aspira volume plus an excess is aspira ted into the tip. ted into the tip. The deliver The deliver y is done y is done
without blow-out, and, thus, the without blow-out, and, thus, the excess volume remains in excess volume remains in the tip. the tip.
The reverse technique also facili The reverse technique also facili tates the tates the repeated delivery repeated delivery of the of the
same volume. If reverse pipetting technique is used the pipette same volume. If reverse pipetting technique is used the pipette
might need recalibration. might need recalibration.
8. 8. 1 1 . For . For ward pipet ward pipet ting (Fig. 6) ting (Fig. 6)
1 1 . . Fit Fit the the tip tip onto onto the the pipet pipet te te tip tip cone. cone.
2. 2. Press Press the the operating operating button button to to the the fi fi rst stop. rst stop.
3. 3. Place the Place the tip tip just under just under the the sur sur face of face of the liquid the liquid (2-3 (2-3 mm) mm)
and smoothly release the operating button up to the starting and smoothly release the operating button up to the starting
position. Wait one seco position. Wait one seco nd. Carefully withdraw the tip from the nd. Carefully withdraw the tip from the
liquid, touching against the edge of the container to remove liquid, touching against the edge of the container to remove
excess from the outside of the excess from the outside of the tip. tip.
4. 4. Dispense the Dispense the liquid liquid by by pressing the pressing the operating button operating button to to the the
fi fi
rst stop. After a shor rst stop. After a shor t delay press the operating but t delay press the operating but ton to the ton to the
second stop. This action will empty the tip. second stop. This action will empty the tip.
Fig. Fig. 6. 6. 0 0
1 1
2 2
Fig. 5. Fig. 5.