Biohit 728010, Instruction Manual

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CONTENTS CONTENTS
1. 1. INTEN INTEN DED DED USE USE ....... ....... ....... ....... ...... ...... ....... ....... ...... ...... ....... ....... ....... ....... ...... ...... ...... ...... 2 2
2. 2. PRODUCT PRODUCT DESCRI DESCRI PTION PTION ...... ...... ....... ....... ...... ...... ....... ....... ....... ....... ...... ...... ... ... 2 2
3. 3. BIOHIT OPTIFIT TIPS BIOHIT OPTIFIT TIPS ....... ....... ...... ...... ....... ....... ....... ....... ...... ...... ....... ....... ...... ...... .... .... 3 3
4. 4. UNPA UNPA CKIN CKIN G G ...... ...... ....... ....... ....... ....... ...... ...... ....... ....... ...... ...... ....... ....... ....... ....... ...... ...... ..... ..... 3 3
5. 5. PIPETTE PIPETTE HOLDER HOLDER AND CAR AND CAR OUSEL ST OUSEL ST AND AND .... .... ... ... .... .... 4 4
6. 6. CALIBRA CALIBRA TION TION TOOL TOOL (ALSO TUBE (ALSO TUBE OPENER) OPENER) ... ... ... ... .... .... 4 4
7 7 . . OPERATING OPERATING THE THE PIPET PIPET TE TE ...... ...... ....... ....... ....... ....... ....... ....... ....... ....... ...... ...... 5 5
7 7 . . 1 1 . . Set Set ting ting the the volume volume .............................................. .............................................. 5 5
7 7 .2. .2. Sealing Sealing and and ejectin ejectin g g tips tips ....... ....... ....... ....... ...... ...... ....... ....... ...... ...... ... ... 5 5
7 7 .3. .3. Protec Protec tive tive tip tip cone cone fi fi lters ........... lters ........... ....... ....... ....... ....... ...... ...... ..... ..... 5 5
8. 8. PIPETTI PIPETTI NG NG TECHNIQ TECHNIQ UES UES ....... ....... ....... ....... ....... ....... ....... ....... ...... ...... ....... ....... . . 6 6
8. 8. 1 1 . . Forward Forward pipet pipet ting ting (Fig. (Fig. 6) 6) ...... ...... ....... ....... ...... ...... ....... ....... ...... ...... ... ... 6 6
8.2. 8.2. Reverse Reverse pipetti pipetti ng ng (Fig. (Fig. 7) 7) ...... ...... ....... ....... ...... ...... ....... ....... ...... ...... ... ... 6 6
8.3. 8.3. Repetitive Repetitive reverse reverse technique technique ....... ....... ...... ...... ....... ....... ...... ...... .... .... 7 7
9. 9. RECOMMENDA RECOMMENDA TIONS TIONS FOR FOR GOOD GOOD PIPETTING PIPETTING ... ... ... ... 7 7
10. 10. MAI MAI NTENA NTENA NCE ............ NCE ............ ....... ....... ....... ....... ...... ...... ....... ....... ...... ...... ....... ....... ....... ....... 8 8
1 1 0. 0. 1 1 . . Dail Dail y y cle cle anin anin g g of of the the oute oute r r sur sur fac fac e e
of of the the pipette pipette ...... ...... ....... ....... ....... ....... ...... ...... ....... ....... ....... ....... ....... ....... ...... ...... .. .. 8 8
1 1 0.2. 0.2. Cleaning Cleaning and and decont decont aminating aminating the the lower lower
par par t t of of the the pipet pipet te te ....... ....... ....... ....... ...... ...... ....... ....... ...... ...... ....... ....... ....... ....... 8 8
1 1 0.3. 0.3. Sterilization o Sterilization o f f the the pipette pipette ...... ...... ....... ....... ...... ...... ....... ....... ...... ...... .. .. 9 9
1 1 1. TESTING THE PERFORM 1. TESTING THE PERFORM ANCE A ANCE A ND ND
RECALIBR RECALIBR A A TION TION ....... ....... ....... ....... ...... ...... ....... ....... ....... ....... ...... ...... ....... ....... ...... ...... .. .. 10 10
1 1 1 1 . . 1 1 . . T T est est ing ing the the per per for for man man ce ce .................................. .................................. 1 1 0 0
1 1 1 1 .2. .2. Rec Rec alib alib rati rati on on ..................................................... ..................................................... 1 1 2 2
12. TROUBLE 12. TROUBLE SHOOTIN SHOOTIN G ........ G ........ ....... ....... ...... ...... ....... ....... ...... ...... ....... ....... ..... ..... 13 13
13. W 13. W ARR ARR ANT ANT Y Y INFORM INFORM A A TION . TION . ....... ....... ....... ....... ...... ...... ....... ....... ...... ...... .. .. 1 1 4 4
1 1 4. PERFORMAN 4. PERFORMAN CE CE SPECIFICATIONS SPECIFICATIONS ....... ....... ....... ....... ....... ....... ... ... 1 1 4 4