pixl Real-Time PCR Platform
Instructions for Use Manual
Page 28 of 37
pixl Real-Time PCR Instructions for Use Manual
Rev. 01-28JUL2021
If any of these parameters fall below a certain threshold, the data is judged as false positive.
The threshold of these parameters is adjustable by the user. Please note this analysis is only
performed on reaction data that is initially judged as positive.
The user can adjust the threshold values and save them with the experiment file or
template file. As shown below, in “Settings”->” Imaging settings” tab the user can
selectively adjust the threshold values (
Figure A6
).
The default threshold values are:
●
Relative strength threshold: 17.5%
●
Amplification efficiency threshold: 20%
●
Signal to noise threshold 12.5
Figure A5
. Determination off false positive amplification curves
.