pixl Real-Time PCR Platform
Instructions for Use Manual
Page 18 of 37
pixl Real-Time PCR Instructions for Use Manual
Rev. 01-28JUL2021
Running amplification program
When running normal qPCR amplification program, the amplification window will monitor
the real time fluorescence signal from the samples. In addition, the Run-Amplification Curve
page will also show the current run status and estimated remaining run time.
Analysis
There are two analysis pages: Amplification analysis (
Figure 11
) and Standard curve analysis
(
Figure 12
). The latter is used for determining the quantity of the DNA/RNA targets using
standard curve analysis method.
Amplification analysis
This will show the amplification curves for all samples.
Ct Threshold
The threshold for determining Ct is expressed as the percentage of the saturating level of
the amplification levels after normalization. The default Ct threshold is 8%.
Ct Low limit
The minimum value of Ct is set through this parameter. This setting is helpful in determining
the base fluorescence value for amplification curve analysis.
Normalize
When displaying amplification curve, this will allow for visualization of Ct threshold value.
This choice will not affect the result of the analysis. An automatic process of Relative
Fluorescence Unit (RFU) normalization takes place after the run is completed.