Biogal PCRun Manual Download | Manualshive

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Summary of Contents for PCRun

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Page 3: ...action General Feature Safety Precautions User Responsibility Analysis of Results Reset PCRun Reader Aborting a Run Downloading Files from the PCRun Reader Deleting Files from the PCRun Reader Analyzing PCRun Graphs Instructions for Use of the PCRun Reader Simulator 5 8 6 9 10 7 13 14 15 16 19 Unpacking and Assembling Operation Guide Page ...

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Page 5: ...ing and Assembling Fig 4 USB Micro USB connector Fig 2 Power cable Fig 7 Transformer Fig 5 Stylus Fig 1 Reader Fig 3 On off switch Fig 6 USB Portable Memory Stick present only in certain models Contents of Box ...

Page 6: ...ch may be translated into a false positive signal Do not place the Reader on the same table with apparatus that my cause vibrations centrifuge vortex shakers etc Vibrations will result in unstable readings Remove the PCRun Reader Fig 8 from the protective packaging and rest on a solid level surface allowing approximately 25 cm of clearance space above the unit for opening Allow 5 cm distance from ...

Page 7: ...onsible for familiarizing themselves with the product instructions and limitations External factors such as sample quality and preparation as well as testing protocols and laboratory techniques may influence the final results It is the user s responsibility to select the proper sample material which meets the criteria of the chosen test As with any test method utilized for in vitro diagnostics the...

Page 8: ... to using the PCRun Reader make sure that it has been set up according to the Unpacking and Assembling instructions 1 1 Turn on the PCRun Reader using the on off switch located on the power cord Fig 10 Model A or on the back of the Reader Fig 10 Model B Operation Guide 1 Starting the PCRun Reader ...

Page 9: ...or certain kits Prior to using the Reader check the kit instructions to determine which protocol is suitable for your test In the case that a different protocol is recommended the following instructions will explain how to change the program temporarily or to define a new default program Using the stylus activate the Test GENERIC tab Fig 12 The names of the various protocols will appear in the win...

Page 10: ...e the new protocol the default setting press Make default located on the bottom left side of the screen Follow by pressing Select 3 Running a PCRun Reaction 3 2 Once the temperature of the Reader has reached its target 60 C the message will change to Run test The indicator light and the thermometer on the touch screen will change to green The temperature noted above the thermometer will be 60 C Fi...

Page 11: ... and the letters will change The test protocol is located in the top middle section of the screen The time period which has passed from initiation of the run can be seen on the bottom middle section of the screen Fig 17 3 6 Sixteen numbered rectangles are located in the middle section of the screen Each numbered rectangle represents a well in which a reaction can be placed After approximately 5 mi...

Page 12: ...d to the activated sample wells Return to the numbered tab screen by activating Back lower right side of the screen Positive reactions will be seen on the graph screen as the reaction progresses at any time point of the run If a reaction is positive the numbered rectangles on the tab screen will change to red and the time at which the reaction was at its maximum Time to Peak will be noted under th...

Page 13: ...d when the program does not respond as expected It can be found on the lower right side of the Reader Fig 21 To reset the program insert the stylus into the small aperture for 2 seconds Once the stylus is removed the program will appear If the problem continues please contact Biogal for assistance 4 Analysis of Results 5 Reset PCRun Reader Fig 21 In cases when a reaction has been initiated and mus...

Page 14: ...med and are the date in reverse 160503AB is the 3rd of May 2016 The first run of the day will receive the suffix AA and the second AB This coding will continue for each additional run on that specific day 7 Downloading Files from the PCRrun Reader 7 2 Open the Files menu A list of the last files will appear Fig 26 To access the older files activate the Next command A new screen will open containin...

Page 15: ...h to transfer to the portable memory stick Press the USB command bottom of screen Fig 28 The message File copied to memory stick will appear on the screen Fig 28 Fig 27 8 2 Press the Multiple Choice command and then select the file or files which you wish to delete Press the Delete command The Files menu will appear and the deleted files will be missing 8 1 Open the Files menu Fig 15 To access the...

Page 16: ...he graph in Fig 30 may be observed if the PCRun Reader has been placed on a surface which receives mild vibrations At the end of the run the Reader may even interpret some of the peaks as positive if they have a Gaussian shape The recommendation is to move the Reader to a more suitable area and repeat the test Fig 30 The peaks in Fig 31 are clear but to the right of the curve low peaks with a flat...

Page 17: ...ave not been allowed to completely dissolve following the addition of the sample or when there are large bubbles within the reaction mix resulting in separation of the fluid into two phases The PCRun Reader has been designed to return a Time to Peak of the later and higher peak Fig 32 Fig 33 kRLU In incidences that the target gene is found in very low concentrations or the extracted sample contain...

Page 18: ...ader will detect these peaks and return a positive response on the tab screen Fig 35 Fig 35 Fig 36 In order to visualize the low peaks deactivate all of the tabs relating to the strong reactions The y axis will change from kRLU to RLU and the peak will appear as a clear histogram Fig 36 2000 0 4000 6000 8000 10000 12000 Time min kRLU ...

Page 19: ... computer 2 Open the Excel file which you transferred to your computer 3 Activate the Select All Button on the upper left hand side of the sheet Fig 38 4 The entire worksheet will be selected and shaded light grey 5 Copy the selected sheet 6 Open the PCRun Simulator The file consists of a workbook containing 4 worksheets You will see the tabs of the 4 worksheets on the bottom of the open worksheet...

Page 20: ...20 For assistance please contact Biogal Galed Labs by Email info biogal com or Tel 972 4 9898605 ...

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