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User’s Manual of LifePro Thermal Cycler Connection to computer

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View running file

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Run File (A/B) is shown below the device name when device is running file.

y

Double click Run File (A/B) to see running file contents and device running status.

Prompt

Click pause/stop to pause/stop equipment.

Double click history sub-item under System items to view historical information.

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Summary of Contents for LifePro

Page 1: ...cted and patented material Without prior written consent from Hangzhou Bioer Technology Co Ltd any part of the manual shall not be duplicated reproduced or translated into any other language File No BYQ60630000000ESM File version July 10 2009 Version 1 0 Before initial use of this instrument please carefully read this manual ...

Page 2: ...he socket shall be correctly fixed by electrician otherwise the earth plug would lose safe protection function b Keeping apart from the live circuit The operator shall not disassemble instrument without permission Only qualified professionals are allowed to replace elements or adjust the machine inside Replacement of elements under energizing state shall be prohibited c Use of power supply Before ...

Page 3: ...aged y The function of instrument obviously changes 3 Instrument label Warning The instrument is adhered with mark HOT SURFACE During operation of instrument or a period immediately after completion of running of a program any portion of body shall not be contacted with metallic portion module or hot lid nearby the said mark otherwise skin would be burnt 4 Maintenance of instrument In order to ens...

Page 4: ...le temperature distribution features 13 2 How to input output PCR program 14 2 1 Connect to USB flash memory 14 2 2 Input file from USB flash memory 14 2 3 Output file to USB flash memory 15 Chapter V Connection to computer 16 1 Software installation 16 2 Online setting 16 2 1 Tool software installation 16 2 2 Device configuration 17 2 2 1 Connect the device to computer by network cable cross B ty...

Page 5: ...ranean anemia hemophilia BMD DMD cystic fibrosis and etc y Immunology HLA typing T cell receptor or antibody diversity qualification autoimmune disease gene mapping lymphokine quantification and etc y Human genome project DNA mark generated from scatter repetitive sequence genetic map construction detect DNA polymorphism sperm drawing physical map construction sequencing expression map and etc y F...

Page 6: ...Cycler Introduction 2 different PCR experiments and rank top in similar products LifePro Thermal Cycler is attached with 1 0 2 ml standard centrifugal tube 2 Strip of 8 tube 3 Strip of 12 tube 4 Full half apron plates ...

Page 7: ...ns Ambient temperature 20 C 55 C Relative humidity 80 3 Basic parameters Specification TC 96 G H b A Sample size 96 0 2ml 96 micro plate 12x8 test tube bar 8x12 test tube bar Scope of temperature 4 C 99 C Heating Rate max 4 0 C Cooling Rate max 4 0 C Temperature uniformity 0 2 C 55 C Temperature control precision 0 1 C Temperature fluctuation 0 2 C Maximum gradient 30 C Minimum gradient 1 C Scope ...

Page 8: ...d 99 respectively The scope for gradient temperature setting is 1 C 30 C b Save the file c Revise the file adjust the rate for temperature rise fall refine temperature and time for cycling automatically and display and calibrate the time d Display the temperature time cycle times setting and real time value at file running including sound prompt total rest time estimation for file running failure ...

Page 9: ...rt Please get familiar with the contents in this chapter for the first time to use this instrument 1 Structure diagram Note Hot lid Keyboard LCD screen USB interface Ventilation hole Block RJ45 interface Power switch Power socket with fuse Caution The fuse specification must be 250V 8A Φ5 20 Use qualified product to replace the fuse Contact fuse supplier or manufacturer when necessary ...

Page 10: ...us for program temperature editing and time refining Cursor left right key press once to move the cursor left right Cursor up down key press once to move the cursor up down Confirmation key press button to accept the setting value on screen at file setting Menu key press button to accept the item above the button on screen Character key to input characters press button continuously to input letter...

Page 11: ...e the power line pug has inserted in power socket reliably 3 Earth the power line reliably 5 Start Power on turn power to the amplifier gives a du du sound and the fan starts to run which indicates the power supply is available Self testing is shown on screen and then the instrument takes about 1 to 2 minutes for self testing Please wait with patience The main interface appears if no problem is fo...

Page 12: ...the unlocking device by finger turn the knob at the opposite direction for two steps and then rotation knob on unlocking device can be released 1 How to run PCR program Power on the thermal cycler gives a du du sound which indicates the power supply is available Self testing is shown on screen and the instrument takes about 1 to 2 minutes for self testing Please wait with patience The main interfa...

Page 13: ...he interface for file running 1 1 How to edit PCR program Press File in main interface to access the interface for file list As shown in figure there are 3 files in the file list Use the up down key to select user when blinking bar blinks in the User column and the file for selected user is shown in the File Name column shift to File Name by right click then the blinking bar blinks in the File Nam...

Page 14: ...erature rise fall Ramp temperature increment in each cycle Temp and time increment in each cycle Time Press Delete to delete the segment where cursor is Press Cycle key for paragraph editing where you can set cycle amount and cycle starting segment The figure shows that it cycles from segment 1 to segment 3 and cycles for 10 times Repeat to press Cycle key to add paragraphs maximum is 5 Press Dele...

Page 15: ...Run to bounce the dialog box below if Control Mode is Tube now It is the same with main interface confirm the password input the sample type and micro liter amount of sample size Caution 1 File name and user name are not case sensitive 2 Please remember the password 3 The password should be 0 9 numeric characters As the file runs normally at Now Running keeps blinking The system shows File run ove...

Page 16: ... indicates the default temperature control mode after system start including Block and Tube choices a Block mode only concerns the temperature control for module during temperature rise fall Now that the temperature rise fall of reagent lags behind the module the actual temperature change of reagent is different from the program we set The actual time that the reagent reaches the set temperature i...

Page 17: ... parameter above Caution 1 Press Save to confirm the system parameter setting or the system parameter won t change 2 It calibrates the clock inside the instrument as per Beijing time upon ex factory 3 It is recommended to check the date and time again for the first time to use 1 3 Gradient module temperature distribution features The gradient module uses 3 line temperature control unit The gradien...

Page 18: ... at left shows press Back to return to main interface 2 2 Input file from USB flash memory Press Back to return to interface of USB flash memory connection Press cursor up down to select copy file user Turn page by cursor left right if user number is more than 30 Press View to see USB file list Hotlid off 2009 05 1 10 05 00 User1 User2 Press Left Right Key to Previous Next Page Back View Hotlid of...

Page 19: ...ile If copy is correct it shows the dialog box below If not it shows Caution 1 USB reads writes 500 users each user has 2 000 files 2 USB reads writes bioer file at USB root directory in fixed directory 3 Each user is the next subfolder under bioer file 4 Don t plug USB flash memory in out quickly To protect device and USB flash memory from damage the device will terminate the USB function until n...

Page 20: ...tion program which is able to control 30 LifePro instruments on one computer 2 Online setting LifePro is equipped with embedded online module inside and tools for setting are available 2 1 Tool software installation Step 1 click and run nead_setup exe Step 2 click Next Step 3 select destination location click Next Step 4 Click Next Step 5 click Install Step 6 complete installation click Finish ...

Page 21: ...nection to computer 17 2 2 Device configuration 2 2 1 Connect the device to computer by network cable cross B type connection 2 2 2 Run Network Enabler Administrator network cable compute device configuration search button mapping function list ...

Page 22: ...d the tool searches instrument automatically Double click the network number in the list column that it searched open the property page and select network items Open the property page select the Operating Mode items click the settings button instrument network number it searched input IP address input subnet mark ...

Page 23: ...s Manual of LifePro Thermal Cycler Connection to computer 19 Select TCP Server Mode under Operating Mode Click OK to complete IP address setting Click the COM Mapping in function list Select TCP Server Mode ...

Page 24: ...computer 20 Click the add target device button to add target device Double click the network number setting property mapping page it searched in list column and select Advanced Settings setting as shown below confirm add target device remove target device ...

Page 25: ...and complete mapping setting Caution 1 Several devices online can t use a same IP address 2 Configure the device again if this instrument is used for the first time 3 Set same subnet for several devices online 4 The device doesn t support the function of acquiring IP address automatically 5 Use switch to connect for several devices online use cross network cable to connect if HUB is used ...

Page 26: ...Select type and volume for test tube y Click OK to run Prompt Select device before running if file is saved in computer Input user Output user Setup user Amend user name Copy user Paste user Delet user Input file New file Copy user Edit file Copy file Delet file Paste file Change file name Change file code Link equipment Save file Disconnect link Grads calculate Equipment setup ...

Page 27: ... y Run File A B is shown below the device name when device is running file y Double click Run File A B to see running file contents and device running status Prompt Click pause stop to pause stop equipment Double click history sub item under System items to view historical information ...

Page 28: ...al Cycler Connection to computer 24 View instrument device storage status Double click System to view instrument device storage status Set system parameters Double click Settings sub item under System items to set system parameters ...

Page 29: ... sub item in device list or any user name sub item in device name sub item y Select create user menu under User menu it bounces a user dialog box y Input user name and click OK Change user name y Select Change user name y Select Change User Name menu under User menu it bounces the dialog box to change user name y Input user name and click OK Prompt User name can t be changed if the file under user...

Page 30: ...under User menu Prompt User can t be deleted if file under user is open Import user y Select local computer y Select Import User menu under User menu y Select the directory for import click Open Prompt User can t be imported if user to be imported is already existed and user file is open Export user y Select user to be exported by local computer y Select Export User menu under User menu y Select t...

Page 31: ...in device list open file list double click edit file or create new file access the interface to edit file y Edit file contents click Save to save file Prompt y All steps can be viewed from graphs y User must input password to edit file if password is required by file Copy file y Select the file to be copied y Select Copy File menu under File menu segment parameters setting setting details cycle pa...

Page 32: ...ile y Select file to be deleted y Select Delete File menu under File menu Prompt File can t be deleted if it is open Change file name y Select file to change name y Select Change File Name menu under File menu y Input file name and click OK Prompt File name can t be changed if it is open Change file password y Select file to change password y Select Change File Password menu under File menu to bou...

Page 33: ... y Select file to import and click Open Prompt File can t be imported if file is already existed and open Export file y Select file to be exported y Select Export File menu under File menu y Select path for export and click OK Prompt File can t be exported if it is open 3 5 Tool menu How to Connect device Disconnect Calculate gradient Change device IP and device name Connect device Select device n...

Page 34: ...initial point and gradient value y Select 8 12 96 holes as calculation module type y Click Calc Change device IP and device name y Select Server Setting item under Tool menu to bounce the dialog box for setting y Click Add to add line y Click Delete to delete current line y Fill in IP address and alias click Save to save setting Prompt y The setting comes into valid only after software restart y I...

Page 35: ...User s Manual of LifePro Thermal Cycler Connection to computer 31 ...

Page 36: ... Power on open instrument press F1 on instrument to access software upgrading preparation status and find Prepare to upgrade on screen 2 Run Network Enabler Administrator program It communicates through virtual device mapping while instrument upgrading 3 Configuration Click search button the tool searches device automatically configuration mapping search button function list network cable compute ...

Page 37: ...ler Firmware upgrading 33 Double click the network number searched on list column open the property page and select network Open the property page select Operating Mode and click setting Network device number it searched ...

Page 38: ...LifePro Thermal Cycler Firmware upgrading 34 Select Real com Mode under Operating Mode choices Click COM MAPPING in function list Select Real Com Mode ...

Page 39: ...LifePro Thermal Cycler Firmware upgrading 35 Click button for add target device confirm remove target device add target device ...

Page 40: ...etting as shown below Click OK click confirmation and apply the setting 4 Run Upgrade exe 5 Press open select upgrading program bin in computer 6 Press start the computer begins to upgrade software in instrument and the instrument screen prompts Upgrade begin 7 The instrument prompts Configuring Mode during software upgrading 8 The instrument prompts Upgrade finished when software upgrading is fin...

Page 41: ...Click remove device remove device mapping 11 Recover the configuration Open Network Enabler Administrator select configuration function Double click network number searched in list column open property page and select Network Select TCP Server Mode under Operating Mode configuration ...

Page 42: ...nistrator 12 Restart the instrument access interface for instrument system parameter setting and set system parameters again Caution Program version might upgrade without notice User can visit www bioer com cn to check and download the latest program version Select TCP Server Mode ...

Page 43: ...ning and Heat sink sensor disconnect is prompted Heater sensor is damaged or in poor contact Contact supplier or manufacturer 5 Please check error is shown on screen while file running and Hotlid sensor disconnect is prompted Hot lid sensor is damaged or in poor contact Contact supplier or manufacturer Ventilation hole is blocked Remove things that block ventilation hole Connection wire is loose O...

Page 44: ...n on reaction tube try to place symmetrically 12 Reagent in reaction tube evaporates Reaction tube is not covered tightly Please cover reaction tube tightly before put into instrument 13 System parameter setting is invalid It didn t press Save to confirm system parameter setting Please refer to 1 2 of Chapter IV in this manual Caution 1 It is forbidden for user to open the case and check by onesel...

Page 45: ...LifePro Thermal Cycler Attachment1 41 Attachment 1 Wiring diagram This diagram is for reference only and subjected to change without notice ...

Page 46: ...h X any segment number already existed 6 to 00 Last segment in this paragraph X 15 Cycle1 00 From 00 to 00 7 Grad Gradient temperature Celsius 0 30 8 Date Date YY MM DD 00 99 01 12 01 31 Year month day 9 Time Time HH MM SS 00 00 00 23 59 59 Hour minute second 10 Default File Default file name in system 11 characters in maximum length 11 Default User Default user name in system 11 characters in max...

Page 47: ...r Attachment 2 43 Hot lid off Off 14 Holid Hot lid on C 30 110 15 Key Sound Key tone yes no Yes No Buzz 16 Run End Sound Prompt tone at running finish yes no Yes No Continuous buzz Fig 1 module reagent Fig 2 module reagent ...

Page 48: ...Memorandum ...

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