GeneQuant
TM
1300 Quick Reference Guide Page 8
Version 2.0
Parameter
Folder
Sub-Folder
Manual
page
Description and options
Units
App
lications
Life Science
Concentration
Kinetics – Parameters 2
Standard Curve
Protein – BCA
Protein – Bradford
Protein – Lowry
Protein – Biuret
45
50
53
30
33
36
39
Enter the units using the alphanumeric keys
or press
::;
and select pre-defined units
using the left and right arrows (options:
(µg/ml, µg/µl, pmol/µl, mg/dl, mmol/l, µmol/l,
g/l, mg/l, µg/l, U/l, %, ppm, ppb, conc or
none)
Units
Life Science OD600
25
Select the units of measurement: OD or
cells/ml
Volume
App
lications
Life science
Absorbance Ration –
Parameters
DNA
RNA
Oligo
Cy Dye
Protein – Protein UV
58
13
15
17
23
28
Enter the volume of the sample. Range:
0.01 to 9999
Volume
Life science Cy Dye
23
Enter the volume of the probe being used in
µl
Wavelength
Applications Concentration
45
Enter the wavelength at which you want to
do the colorimetric assay
Wavelength
Applications Kinetics – Parameters 1
50
Enter the wavelength at which you want to
measure absorbance over a period of time
Wavelength
Applications Single wavelength
43
Enter the wavelength at which you want to
measure absorbance or % transmission
Wavelength
Applications Standard curve
53
Select the wavelength at which you want to
construct the calibration curve
Wavelength
Life science Cy Dye
23
Enter the wavelength of the dye absorbtion
peak
Wavelength
Life Science OD600
25
Select the wavelength. Default value is 600
nm
Wavelength
Life Science Protein – BCA
30
Set at 562 nm
Wavelength
Life Science Protein – Biuret
39
Set at 546 nm
Wavelength
Life Science Protein – Bradford
33
Set at 595 nm
Wavelength
Life Science Protein – Lowry
36
Set at 750 nm
Wavelength 1
Applications Absorbance Ratio –
Wavelengths
58
Enter the first wavelength which you want to
use to measure the absorbance ratio
Wavelength 2
Applications Absorbance Ratio –
Wavelengths
58
Enter the second wavelength which you
want to use to measure the absorbance
ratio
Wavelength 3
Applications Absorbance Ratio –
Wavelengths
58
Enter the wavelength from which the
background correction will be obtained. This
parameter is only available if the
background parameter has been set to On
Wavelengths
Applications Multi Wavelength
57
Select the number of wavelengths at which
you want to measure absorbance. Range 2-
5
X axis limits
Applications Wavescan, options 6 -
Graph Scale
49
Set to On to define the start and finish
points of the x axis, or off to retain default
values
X1
Applications Wavescan, options 6 -
Graph Scale
49
Enter the minimum value for the x axis
X2
Applications Wavescan, options 6 -
Graph Scale
49
Enter the maximum value for the x axis