biochrom GeneQuant 1300, Quick Reference Manual

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GeneQuant
TM
1300 Quick Reference Guide Page 4 Version 2.0
Parameter Dictionary
Parameter Folder Sub-Folder Manual
page
Description and options
A Life Science Nucleic Acids - Oligo 17 Enter the proportion of Adenine bases.
Default is 10, range: 0 – 9999. Only an
option when units are pmol/ µ l
A260 Life Science Protein – Protein UV 28 Enter coefficient 2 (for absorbance at 260
nm). Default is 0.76 as in Christian and
Warburg equation: protein (mg/ml) =
1.55*Abs 280 – 0.76*Abs 260
A280 Life Science Protein – Protein UV 28 Enter coefficient 1 (for absorbance at 280
nm). Default is 1.55 as in Christian and
Warburg equation: protein (mg/ml) =
1.55*Abs 280 – 0.76*Abs 260
Autodetect
peaks
Applications Wavescan 48 Yes/No – turns on and off the automatic
peak detection
Auto-Print Utilities Printer 62 Select whether auto-print is on or off. When
on, results are automatically printed after a
measurement is taken. When off, printing
has to be initiated manually
Auto Standby Utilities Preferences 63 Select whether to use a standby mode after
defined periods. Options: 1 hour, 2 hours, at
night or off
Background Applications Absorbance Ratio –
Wavelengths
58 Select whether a background correction is
applied to both wavlengths
Background Life Science Nucleic acids – DNA
Nucleic acids – RNA
Nucleic acids – Oligo
Cy Dye
Protein – Protein UV
13
15
17
23
28
Select whether the background correction
at 310 nm is used or not. Options: On or Off
Base sequence Life Science Tm Calculation 21 Enter the base sequence triplets using the
annotated number keys. 1=A, 3=C, 4=G,
6=T/U
Base Type Life Science Tm Calculation 21 Select the base type: DNA or RNA
Brightness Utilities Contrast 63 Adjust the brightness using the left and right
arrows
Buffer molarity Life Science Tm Calculation 21 Enter the molarity of the buffer.
C Life Science Nucleic Acids - Oligo 17 Enter the proportion of Cytosine bases.
Default is 10, range: 0 – 9999. Only an
option when units are pmol/ µ l
Calibration App lications
Life Science
Standard curve
Protein – BCA
Protein – Bradford
Protein – Lowry
Protein – Biuret
53
30
33
36
39
Select the calibration mode. Standard,
measure prepared standard or Manual,
enter values using key pad numbers
Coefficient Life science Cy Dye 23 Use to enter the exitinction coefficent of the
dye
Contrast Utilities Contrast 63 Adjust the contrast using the left and right
arrows
Correction Life Science OD600 25 Enter the correction factor to compensate
for different optical configurations between
this and other instruments. Default value is
2
Counter ion Life Science Tm Calculation 21 Select the counter ion: Na, K, TEA or Other