BioCare Medical Decloaking Chamber NxGen, Manual

Page: 9 / 24

6
Instructions for Use
Operating Instructions
Once the Decloaking Chamber NxGen is appropriately set up, antigen retrieval can begin.
1. If not already plugged in, plug the power cord first into the Decloaking Chamber NxGen and then into an appropriate wall outlet.
Turn ON / OFF switch to the ON position (|).
2. To remove lid, grasp handle, turn clockwise and lift. Gently set the lid next to the unit without removing the pressure cable.
3. Add 500ml of deionized or filtered water to the Decloaking Chamber NxGen metal water chamber. Make sure that
the outside of the metal water chamber is kept completely dry and clean and there are no dents or deformations in the
bottom or rim.
Note: Less than 500ml of deionized or filtered water may cause adverse staining results.
4. Assemble slide racks, metal slide canisters and metal rack holder into water chamber.
a. Place slide racks into each metal slide canister.
b. Fill each metal slide canister to the top of the slide rack excluding the handle (200-220 mls) with either HIER or
deionized or filtered water.
c. Place 3 metal slide canisters into metal slide rack. Note: Always use 3 metal slide canisters per run.
If there aren’t enough slides for 3 canisters, fill the other metal canister(s) with deionized or filtered water and
run them as if slides were placed into the canister.
d. Place metal slide rack containing metal slide canisters into the metal water chamber. Ensure the metal water chamber
is positioned so that the white cup-level markings face to the rear of the unit.
e. For 110°C protocols, place a dry Steam Monitor Strip on top of the metal slide canisters or metal slide rack.
5. Before locking the lid, make sure sealing ring is securely in place on the sealing ring supporting cover (left figure below).
Hold knob on sealing ring supporting cover and press firmly over the center post of inner lid to secure (right figure below).
6. Place lid on the Decloaking Chamber NxGen and turn it counterclockwise into position. When placing lid on the Decloaking
Chamber NxGen, the pressure cable should always be towards the back of the unit.
7. Ensure that the pressure cable is securely attached to the lid.
8. Position the pressure limit valve as directed in figures below, ensuring it is set level on the pressure stem, not tipped to one
side. If the pressure limit valve is tipped to one side, proper pressure buildup will not occur. NOTE: The pressure limit valve
does not click or lock into place. Even though it will have a loose fit, it is safely secured.
Do not touch the pressure limit valve when the Decloaking Chamber NxGen is pressurized. Steam and hot water could vent.
9. Select protocol variables as described in Control Panel Programming.