BioCare Medical Decloaking Chamber NxGen Manual Download | Manualshive

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800.799.9499
60 Berry Drive
Pacheco CA 94553

www.biocare.net

904-012-071817

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Summary of Contents for Decloaking Chamber NxGen

Page 1: ...www biocare net U S patent number 6 580 056 Decloaking Chamber NxGen Manual Digital programmable pressure system for heat induced epitope retrieval ...

Page 2: ...Biocare Medical Technical Support 800 799 9499 Option 3 Customer Service 800 799 9499 Fax 925 603 8080 Corporate Office 60 Berry Drive Pacheco CA 94553 www biocare net ...

Page 3: ...Safeguards 4 Before First Use 5 Upon Receipt 5 Initial Set Up 5 Instructions for Use 6 Operating Instructions 6 Control Panel Programming 7 Program Completion 9 95 C Protocol Function 10 Reporting Software 11 USB Flash Drive 11 Software Reports 11 Software User Guide 11 Maintenance 13 Daily 13 Monthly 13 Troubleshooting Guide 14 Quick Instruction Guides 15 Instrument Operation 15 Software Reports ...

Page 4: ... Decloaking Chamber NxGen is designed to optimize and standardize antibody staining procedures and has been engineered to pass strict laboratory safety requirements Temperature pressure and time can be monitored and recorded with the Decloaking Chamber NxGen to produce consistent staining Specifications Intended Use The Decloaking Chamber NxGen is a programmable bench top temperature pressure cham...

Page 5: ...ith a minimum capacity of 512 MB and a maximum capacity of 4 GB Waste Disposal All reagents used in the Decloaking Chamber NxGen must be properly disposed of in accordance with local state and federal regulations Instrument Disposal Dispose of the Decloaking Chamber NxGen in accordance with local state and federal regulations Decontaminate with an antibacterial cleansing agent Accuracy Temperature...

Page 6: ... ON OFF Switch 11 Power Cord 12 Lid Handle 13 Lid 14 Sealing Ring Supporting Cover 15 Rubber Grommet 16 Upper Ring 17 Control Panel 18 Pressure Cable 19 Push Rod 20 USB Port 21 Home Screen a Menu Button b Circle Button c Clock Button 22 Metal Slide Canister 23 Slide Rack 24 Metal Rack Holder DC2012 1 3 4 6 7 8 a b 2 9 10 11 12 18 13 14 15 17 5 23 16 22 24 21 20 c 19 ...

Page 7: ...aking Chamber NxGen will not be able to record the pressure during the run 7 Always check the pressure release device for clogging before use Do not operate without the pressure release properly installed 8 Do not place the Decloaking Chamber NxGen in a heated oven or on any heated surface Do not place the Decloaking Chamber NxGen near a flame or gas source 9 Keep the exterior of the unit dry To p...

Page 8: ... to the ON position 6 Ensure the Decloaking Chamber NxGen is on the Home Screen a Current temperature of the unit b Software version c Consult manual before operating notice 7 Set the current date MM DD YYYY a Push the clock button on the home screen b Use the clock button up arrow to select the current month If you pass the current month continue pushing the clock button and the month will cycle ...

Page 9: ... or filtered water and run them as if slides were placed into the canister d Place metal slide rack containing metal slide canisters into the metal water chamber Ensure the metal water chamber is positioned so that the white cup level markings face to the rear of the unit e For 110 C protocols place a dry Steam Monitor Strip on top of the metal slide canisters or metal slide rack 5 Before locking ...

Page 10: ...g the clock button and it will cycle back to 00 For faster cycling hold down the clock button d Use the menu button right arrow to move to the minute selection It will become highlighted e Use the clock button up arrow to select the next variable f Continue in this fashion until all variables have been programmed g Press the circle button to save the temperature selection There will be a pause of ...

Page 11: ...or is cooling down down arrow iii The selected protocol number protocol temperature and protocol time b In the middle of the screen the run screen displays iv A graphical representation of where the unit is at in the current run as represented by the circle on the graph iii i v ii vi vii iv c In the bottom of the screen the run screen displays the following v Unit current pressure in PSI vi It wil...

Page 12: ... and the water replaced with room temperature deionized or filtered water to expediate the cool down 5 Use extreme caution when opening the lid Turn the lid clockwise and lift Tilt the lid at a 45º angle above the water pot and shake several times This will allow the water that has accumulated in the lid to drain Place the lid next to the unit a Use extreme caution when opening the lid Liquids and...

Page 13: ...re has been achieved The following message will be displayed pictured below on the unit screen with two separate options for user input 1 If slides will be added to the hot retrieval solutions press the button under the Load slides option and open the unit to add the necessary slides Replace and secure lid The run will resume automatically with the user selected hold time after the unit temperatur...

Page 14: ...e on the selected instrument If run data was collected from multiple Decloaking Chamber NxGen the Summary Report can be configured to list the run data from a single instrument or for all associated units at once Summary Report by Date The summary report by date generates a list of all of the runs performed between two user selected dates If run data was collected from multiple Decloaking Chamber ...

Page 15: ...Click on the Summary Report by Instrument option 2 In the Serial option window select the serial number of the desired Decloaking Chamber NxGen from the drop down list Alternatively leave the Serial option set to All to generate a Summary List of all uploaded Decloaking Chamber NxGen run files 3 Use the drop down calendar in the Start Date and End Date option window to set the desired start and en...

Page 16: ...in water or pour water into it 4 With warm water rinse the underside of the lid including the sealing ring pressure limit valve anti block cover air escape and pressure indicator poppit Dry completely 5 Clean area under upper ring with dampened cloth or microfiber cloth Do not use chemical cleaners 6 To clean sealing ring hold the knob on the sealing ring supporting cover and pull the sealing ring...

Page 17: ...p lid to loosen the poppit and allow it to rise The metal water chamber may be dented or deformed Contact Biocare Technical Support Display is not showing anything Unit is not plugged in Plug the power cord into the unit and then into the wall outlet ON OFF switch is in the OFF 0 position Check to see if switch is in the ON position Message Appears ERROR No water in water chamber Put 500ml of deio...

Page 18: ...mpletion and unit is under 95ºC carefully open the lid Tilt the lid at 45º and gently shake to drain excess water before fully removing the lid Use extreme caution as steam and liquids can still be very hot 10 Remove metal rack holder by grasping the plastic handles Do not grab the metal rack holder or the metal slide canisters by the metal it is very hot 11 Continue with slide processing 12 Once ...

Page 19: ...ure sensitive steam strip allows the end user to monitor both pressure and heat inside the Decloaking Chamber NxGen The best temperature for a Steam Monitor strip to show quality control is 110ºC 5ºC It is at this temperature and relative pressure that a dark brown to black color is achieved Lower temperature and pressure will produce a lighter strip The steam strip can be dated and recorded for l...

Page 20: ...ropean Community ETL Approved Manufacturer Serial number Hot Surface Consult instructions for use Caution consult accompanying documents Power USB connector Power socket Symbol Glossary Please contact Biocare Medical Technical Support if any problems arise 800 799 9499 Option 3 ...

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Page 24: ...800 799 9499 60 Berry Drive Pacheco CA 94553 www biocare net 904 012 071817 ...

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