Biobase BK-AutoHS96 User Manual Download Page 40

39

As shown in Figure 4-11:

1

Open the left cabinet door of the instrument lower cabinet and take out the waste

tips bag

Base from the drawer.

2

Snap the waste tip bag into the base, and open the waste tip bag;

3

Put the base of the waste tip bag back into the drawer and push the drawer

to a fixedposition;

4

Close the lower cabinet door.

1.

waste tips bag 2.base of the waste tip bag 3.left door of the lower cabinet

Figure 4-11 Waste tip bag loading (one step method)

4.4.3.2 Load reagents and consumables for magnetic bead testing

Load 5mLCryovial

As shown in Figure 4-5 and Figure 4-12:

Place the 5mL cryopreserved tube with the reaction solution PCRmix in the No. 4 hole in the

cryovial rack;

After loading the cryopreservation tube, please check whether the position is placed correctly

and securely, and handle it if there is any abnormality;

Figure 4-12 5mL cryopreservation tube loading (magnetic bead method)

Load 100mL Reagent tank

As shown in Figure 4-5 and Figure 4-13:

1

Place the 100 mL reagent tanks with the extraction solutions 1, 2, 3, and 4 in

the reagent tank carrier at positions 1, 2, 3, and 4;

Summary of Contents for BK-AutoHS96

Page 1: ...Automatic Nucleic Acid Extraction System BK AutoHS96 User Manual BIOBASE GROUP Version 2020 07...

Page 2: ...duct outline structure 15 2 3 1 Product external structure 15 2 3 2 Internal structure 18 2 3 3 Product Consumables 20 2 4 Instrument installation 23 III Product software description 25 3 1 Software s...

Page 3: ...5 2 Equipment alarm and handling measures 47 5 3 Handling when the instrument is deactivated 47 VI Product maintenance 49 6 1 Detergent 49 6 2 Daily maintenance 49 6 3 Monthly maintenance 50 6 4 Annua...

Page 4: ...consumables used for replacement and maintenance are provided or accepted by Biobase Biodustry Shandong CO ltd The relevant electrical equipments comply with national standards and the requirements of...

Page 5: ...d command of the operation knowledge before using the instrument Note The user manual is intended for the following professionals Daily operation personnels Maintenance and troubleshooting personnels...

Page 6: ...ion manual Telectric shock hazards Please refer to the detailed instructions on the Operator Manual and operate carefully to prevent electric shock Be careful to pinch and prick Please refer to the de...

Page 7: ...Text description Text Description Note It is used to explain important information in the operation steps or other contents that needs to remind the user WARNING Remind the user to follow the instruc...

Page 8: ...nded via ground wire The power ground wire must be grounded to avoid electric shock The AC power supply must be stable It is forbidden to share the power supply with high power appliances Don t touch...

Page 9: ...r At the same time the instrument maybe damaged Please note the following possible mechanical hazards Injury caused by mechanical arm squeeze Injury caused by the pipette tip Protection against fire a...

Page 10: ...9 measures should be taken to reduce interference Biohazard prevention Please observe the following precautions to prevent biohazard effectively...

Page 11: ...le it and consult a doctor Waste disposal To prevent environmental pollution and personal injury caused by waste products please observe the following precautions Biohazard Follow the relevant local l...

Page 12: ...ematic laboratories 1 1 Basic Product Information Product Name Automatic Nucleic acid extraction system Product model BK AutoHS96 1 2 Product Scope This product is intend for extract nucleic acid from...

Page 13: ...include sample carrier reagent carrier gun head carrier sample loading arm grasping arm oscillation heating module refrigeration mod Le magnetic module barcode scanning module protection system transp...

Page 14: ...ration pollution large noise sources and electromagnetic interference For indoor use avoid direct sunlight or place it in front of heat and wind sources Choosea well ventilated location Ambient temper...

Page 15: ...14 1 The reserved space between the right side of the instrument and the wall or adjacent object is 50cm 2 The reserved space between the left side of the instrument and the wall or adjacent object is...

Page 16: ...matic Nucleic acid extraction system BK AutoHS96 is consisted of sample carrier reagent carrier tips carrier sample loading arm grasping arm oscillation heating module cooling module magnetic module b...

Page 17: ...tion 1 Security door For safety protection 2 Touch screen To display the operation interface 3 Power switch For internal power supply of the instrument 4 Lighting switch Provides interior lighting 5 L...

Page 18: ...17 10 Rear service panel For mechanical arm maintenance...

Page 19: ...tion 14 USB Used for communication 15 Computer forced switch Long press for 5 Seconds to force shutdown computer 16 Computer power indicator To display the computer is powered on or off 17 Computer ru...

Page 20: ...19 Figure 2 3 Internal Structure of Automatic Nucleic acid extraction system BK AutoHS96...

Page 21: ...e Nucleic acid isolation and purification 8 Deep hole plate Place the deep well plate 9 Reagent tank carrier Place the reagent tank 10 Refrigeration module Temperature control of deep well plate solut...

Page 22: ...System BK AutoHS96 No Name schematic diagram Function 2 200 L tip Liquid distribution 3 96 deep well plate Solution storage and reaction 4 Reagent tan k carrier Storage reagent 5 cryopreserva ti ontub...

Page 23: ...22 7 Waste tip bag Collect abandoned tips...

Page 24: ...ot cup Figure 2 4 Automatic Nucleic Acid Extraction System BK AutoHS96 installation structure diagram 1 Open the shipping outer packaging of the instrument 2 Open the door of the bottom cabinet of the...

Page 25: ...two doors of the lower cabinet and adjust the height of the foot cup at the screw hole of the foot cup with the screw cup tool and ratchet wrench until the foot cup holds the entire instrument up the...

Page 26: ...quipment self test etc in this interface AutoNo Each sample is automatically numbered in order from the starting number Submit Select the experimental program input the number of samples and the numbe...

Page 27: ...26 numbering function it is used to define the number of the first sample...

Page 28: ...nthistory query Run an experiment to save a historical record Search You can select the program name program type experiment state operator and start and end dates of the experiment and Then click to...

Page 29: ...file for the experiment completion sound Default QC Qty Set the default QC quantity This quantity is the sum of the number of standards and QCs placed on the standard seat When it is set to empty you...

Page 30: ...elay input minutes the UV lamp will start according to the set time delay if you set the on time the UV lamp will light up and wait for the set time to automatically turn off the UV lamp This setting...

Page 31: ...on when the software starts Turn off Fan Automatically It is used to set the exhaust fan to turn off when the software exits Turn on Blue Lamp Click to turn on the blue lamp Turn off Blue Lamp Click...

Page 32: ...re version and copyright information 3 7 Helpful information 1 1 Helpful information interface In this interface you can click to view the Instrument Operation Manual and software version Help Click t...

Page 33: ...Exit Click the Exit button and the Exit System dialog box will pop up OK Click to exit the software system If you select Turn on the UV lamp the UV lamp will be turned on and off according to the set...

Page 34: ...before instrument start up Figure 4 1 Experimental flowchart 4 2 Check before starting Be sure to check the instrument carefully before the experiment Notice Make sure that the power cord and communi...

Page 35: ...shown in Figure 4 2 The boat shaped switch 1 means on and the indicator light is on 0 means off and the indicator light is off Instrument power switch as shown in Figure 4 3 and 4 4 which is a key loc...

Page 36: ...action solutions 1 3 and 4 to 100mL reagent tanks respectively and mark them accordingly 2 Mix the configured extraction solution 2 with shaking centrifuge transfer it to a 100mL reagent tank and mark...

Page 37: ...drawer to the limit position 5 Check that the drawer is pushed back into place Figure 4 5 Reagent tank and cryotube carrier area map Figure 4 6 5mL cryopreservation tube loading one step method Load 9...

Page 38: ...the packing box with the arc notch on the plate rack aligned with the cylindrical pin on the tip carrier and the board rack is clamped between the two elastic pieces so that the lower plane of the boa...

Page 39: ...00 L tips according to experimental needs 5 Push the tip carrier drawer back to return the drawer to the limit position 6 Check that the drawer is pushed in place Figure 4 9 200 L tip loading one step...

Page 40: ...ne step method 4 4 3 2 Load reagents and consumables for magnetic bead testing Load 5mLCryovial As shown in Figure 4 5 and Figure 4 12 Place the 5mL cryopreserved tube with the reaction solution PCRmi...

Page 41: ...tep reagent Load 8 tube strips 1 Please refer to load 8 tube strip in the one step reagent Load waste tip bag 1 Please refer to load waste tip bag in the one step method Load waste tank As shown in Fi...

Page 42: ...1000 L tip 1 Please refer to load 1000 L tips in one step reagent Load 8 tube strips 1 Please refer to load 8 tube strips in the one step reagent The unified extraction needs to determine the number o...

Page 43: ...mental program from the experimental program drop down list 2 Input the number of samples for the current experiment 3 Input the quality control quantity 4 Select the execution start block and the exe...

Page 44: ...he reaction solution and prompting the sample nucleic acid to be retained 3 Completing the experiment 1 Click OK in the experiment task completion dialog box and the experiment project is completed 4...

Page 45: ...reagent bottle and tip plate rack intothe bag after the experiment 4 Properly dispose of medical waste in accordance with the laboratory biosafety system 5 Clean the instrument table and perform nece...

Page 46: ...ents to avoid splash and spillage of the liquid on any exposed parts of the body 5 When the instrument is finished or not used for a long time turn off the instrument and shut down the power 6 All par...

Page 47: ...asons Contact a service technician tip can t take off The instrument is not operating in the operating environment required by the instrument temperature is too low Adjust the laboratory environment t...

Page 48: ...solution After adding an appropriate amount of specimen reagent or control solution click Retry or ReGetTips Retry Aspiration does not move to the lowest position Click on Asp to lowest pos Dosing arm...

Page 49: ...tles etc on the platform in time Notice Operation not in accordance with this operation manual may result in malfunction If the problem cannot be solved by the solutions listed in this chapter or is n...

Page 50: ...ly test check the instrument table to keep the platform clean and free of residual liquid and dirt If there is liquid remaining use a blotting paper to remove the liquid first and then wipe the surfac...

Page 51: ...ule Note Annual equipment maintenance must be performed by a BIOBASEafter sales service engineer When the product needs to be reused after out of device for a long time there may be problems such as i...

Page 52: ...51 Acid Extraction System or individual parts are...

Page 53: ...ealer or manufacturer in need the necessary cleaning and disinfection must be performed The waste liquid produced in the experiment should be checked in time and needs to be poured into the designated...

Page 54: ...53 Appendix Parts List No Component name Specifications Quantity 1 Fuse 6 3A 2...

Page 55: ...nan City Shandong Province China Tel 86 531 81219803 01 Fax 86 531 81219804 Inquiry export biobase com Complaints customer_support biobase cc After sales service service_sd biobase cc service_ivd biob...

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