Section 11
Specifications
11.1 Specifications
Product Specifications
SmartSpec 3000 Spectrophotometer
Wavelength range: UV and Visible ranges
200 to 800 nm
Wavelength accuracy
(+/-) 2 nm
Spectral bandwidth
5 nm
Light source
Xenon flash lamp
Detector
Photodiode array
Number of cuvettes
one
Size of cuvettes
100 µl, 700 µl, 1.5 ml, 3.5 ml
Pathlength
1 mm, 2 mm, 5 mm and 10 mm
Display
2 x 24 alphanumeric built-in backlit LCD
display
Printer
Built-in alphanumeric/graphic
Interface
RS-232 serial port
Operating temperature
5–40 °C
Photometric range
0–2 AU
Scan speed
200–800 nm in 20 seconds max
Photometric repeatability
± 0.02 AU in a 0.1–2.0 AU range
(9 readings) and over a 200–800 nm range.
Dimensions
33 cm (W) x 26 cm (D) x 14 cm (H)
Weight
3.6 k.g.
Power requirement
90–260 VAC, 47–63 Hz
Regulatory
CE, TÜV
11.2 Calculations
I.
Molecular Weight (MW):
•
Length (L): MW
≈
L * 310 - 61
•
Sequence or Composition:
DNA: MW = (#A * 312.2) + (#C * 288.2) + (#G * 328.2) + (#T * 303.2) - 61
RNA: MW = (#A * 312.2) + (#C * 288.2) + (#G * 328.2) + (#U * 289.2) - 61
II. Molar Extinction Coefficient (
εε
):
•
Length:
ε ≈
L * 10,000 liter/mole/cm
•
Composition:
DNA:
ε ≈
(#A * 15480) + (#C * 7340) + (#G * 11760) + (#T * 8850) liter/mole/cm
RNA:
ε ≈
(#A * 15480) + (#C * 7340) + (#G * 11760) + (#U * 9000) liter/mole/cm
•
Sequence: See Optical properties of nucleic acids, absorption, and circular dichroism
spectra, p. 589, CRC Handbook of Biochemistry and Molecular Biology 3rd Edition,
Nucleic Acids - Volume I. Gerald D. Fasman, Ed. CRC Press 1975.
49
