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Section 1 General Information
1.1 Introduction
The Model GS-800 Calibrated Imaging Densitometer is a high performance, calibrated
imaging densitometer that converts transparent and opaque electrophoretic samples into
digital data. These data are analyzed with Quantity One® software operating in either
Microsoft Windows® or Macintosh® computer systems. For a step-by-step guide to
scanning and analyzing images, please refer to your Quantity One software user manual.
The main features of the Model GS-800 Calibrated Imaging Densitometer include:
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Adjustable resolution from 36.3 microns to 127.0 microns;
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Reflectance and transmittance
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Scanning area of 29 cm by 40 cm
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Sampling rate of up to 700 dots per inch (dpi).
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Stationary platen for higher reliability
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Variable wavelength lamp/filter architecture for improved color discrimination
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Analog to digital conversion at full 12-bit accuracy
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Calibrated transmission tablet to ensure accurate OD readings
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Reflective gray scale tablet to ensure reproducible reflectance values
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Supported by Quantity One software, which provides versatile image display,
optimization, and quantitation features
1.2
Installation Qualification and Operational Qualification ( IQ/OQ )
The calibration of the Model GS-800 Calibrated Imaging Densitometer can be validated by
following the GS-800 Calibrated Densitometer Installation Qualification and Operational
Qualification protocols. These protocols are available as an accessory to the GS-800
Calibrated Imaging Densitometer ( Catalog number 170-7956 ). These straightforward and
simple to follow IQ/OQ protocols include instructions for the installation of the hardware
and software, and instructions for the verification of the reflectance and transmittance
calibration functions.
Following proper installation, a linear dynamic range of 3.0 OD for the
Transmissive scanning and 2.0 OD for Reflectance scanning can be confirmed with
greater than 95% confidence.
For additional details, contact your local Bio-Rad
Representative.
