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TC-512

OPERATOR'S MANUAL

Issue 3

3/08

TC512 Techne Manual 19/12/08 3:30 PM Page 1

Summary of Contents for Techne TC-512

Page 1: ...TC 512 OPERATOR S MANUAL Issue 3 3 08 TC512 Techne Manual 19 12 08 3 30 PM Page 1 ...

Page 2: ...D INSTALLATIONSINFORMATIONEN 9 Sicherheit des Bedienpersonals 9 Achtung 9 Auspacken 9 Inbetriebnahme 9 Ersatzkabel 10 Einsatzbedingungen 10 Garantie 11 INFORMACIÓN DE SEGURIDAD E INSTALACIÓN 12 Seguridad del operario 12 Advertencia 12 Desembalaje 12 Instalación 12 Cable de repuesto 13 Condiciones de trabajo 13 Garantía 14 INFORMAZIONI SULLA SICUREZZA E L INSTALLAZIONE 15 Sicurezza dell operatore 1...

Page 3: ...emplate 33 Option 3 Creating a New Program 34 Inserting a Segment 35 Inserting Cycle Numbers 36 Inserting Incremental or Decremental Time or Temperature 36 Inserting a Pause 38 Saving Changes to a Program 38 RUNNING A PROGRAM 38 Program Defaults 41 Data Logs 43 Printing a Data Log 43 Gradient Calculator 45 SYSTEM ADMINISTRATION 46 Program Passwords 47 System Administration Password 47 System Test ...

Page 4: ...ture Heating and cooling rates between the specified temperatures in C sec Gradient of temperatures across the thermal cycling block Incremental time or temperature increases or decreases when a cycle is repeated Before using the TC 512 make sure you have read this manual carefully If there is any doubt relating to the proper use of this equipment the staff at Bibby Scientific Ltd or your supplier...

Page 5: ...heating samples remember that parts of the instrument namely the lid and block and any associated accessories may be very hot UNPACKING When unpacking please ensure that the following have been removed from the packaging Thermal cycler Mains cable Guarantee card Decontamination certificate The user is advised to keep the original packaging in case the instrument ever needs to be returned for servi...

Page 6: ...unit on a suitable flat bench or in a fume cupboard if required ensuring that the air inlet vents on the underside are free from obstruction Position the instrument with a minimum distance all round of 200mm from walls or other items and between each unit if multiple units are being used to permit efficient air flow for each instrument 7 Switch on the instrument I mains switch On O mains switch Of...

Page 7: ...suse and will invalidate the guarantee for this component Servicing under this guarantee should be obtained from the supplier of the instrument This manual has been prepared for the convenience of Bibby Scientific Ltd s customers and nothing in this manual shall be taken as a warranty condition or representation concerning the description merchantability fitness for purpose or otherwise of the uni...

Page 8: ...liser les consommables en plastique appropriés Rappel après le chauffage d échantillons certaines parties de l appareil notamment le couvercle et le bloc ainsi que les accessoires associés peuvent être brûlants DÉBALLAGE Au moment du déballage vérifier que l emballage contenait les articles suivants Cycleur thermique Câble secteur Carte de garantie Certificat de décontamination Il est conseillé à ...

Page 9: ...er le câble secteur dans la prise au dos de l appareil 6 Disposer l appareil sur une paillasse ou sous une hotte aspirante si nécessaire en veillant à ce que les prises d air situées au dessous ne soient pas obstruées Positionner l appareil avec tout autour un écartement minimum de 200 mm par rapport aux murs ou autres éléments ainsi qu entre chaque appareil en cas d utilisation d appareils multip...

Page 10: ...égâts de cette sorte seront considérés comme une mauvaise utilisation et annuleront la garantie de ce composant Dans le cadre de cette garantie le service après vente est à obtenir auprès du fournisseur de l appareil Le présent manuel a été préparé pour le confort des clients de Bibby Scientific Ltd et rien dans son contenu ne doit être pris comme une garantie une condition ou une affirmation conc...

Page 11: ...eiten NICHT direkt auf das Gerät auftragen verwenden Sie stets die entsprechenden Kunststoffeinsätze Nach der Erhitzung von Proben daran denken dass bestimmte Geräteteile wie Deckel Thermoblock und Zubehörteile sehr heiß sein können AUSPACKEN Beim Auspacken darauf achten dass der folgende Lieferumfang vorhanden ist Thermocycler Netzkabel Garantiekarte Dekontaminationszertifikat Wir empfehlen die O...

Page 12: ...rieben werden Bei Geräten für 100V gilt der Bereich 90 bis 110V In allen Fällen reduziert sich die Erhitzungsrate um etwa 8 in den äußeren Spannungsbereichen 5 Stecken Sie das Netzkabel in die Buchse auf der Geräterückseite ein 6 Stellen Sie das Gerät auf einen geeigneten ebenen Tisch oder in einem Abzugsschrank auf und sorgen Sie dafür dass die Lufteinlassschlitze auf der Geräteunterseite nicht b...

Page 13: ...eige des Thermocylers kan durch spitze scharfe Gegenstände wie Stifte Bleistifte und Fingernägel beschädigt werden Diese Art von Beschädigung gilt als Missbrauch des Geräts und führt zur Ungültigkeit der Garantie für dieses Gerät Wartungsarbeiten die unter diese Garantie fallen müssen von der Verkaufsstelle für dieses Gerät gehandhabt werden Diese Anleitung wurde zur Information der Kunden von Bib...

Page 14: ... adecuados Después de calentar las muestras recuerde que hay componentes del instrumento que pueden calentarse mucho concretamente la tapa y el cuerpo y todos los accesorios asociados DESEMBALAJE Durante el desembalaje asegúrese de sacar los siguientes componentes del embalaje Ciclador térmico Cable de alimentación Tarjeta de garantía Certificado de descontaminación Se recomienda guardar el embala...

Page 15: ... dos extremos del intervalo de voltaje 5 Conecte el cable de alimentación en el enchufe situado en la parte trasera del instrumento 6 Sitúe la unidad sobre una mesa plana o en una campana de laboratorio si es necesario y asegúrese de que los orificios de ventilación situados en la parte inferior no tienen ninguna obstrucción Coloque el instrumento a una distancia mínima de 200 mm alrededor de pare...

Page 16: ...grafos lápices y uñas pueden dañar fácilmente la pantalla del ciclador térmico Este tipo de daño se considerará como un uso incorrecto e invalidará la garantía de este componente El distribuidor del instrumento proporcionará información sobre las reparaciones realizadas bajo esta garantía Este manual se ha preparado con una finalidad informativa para los clientes de Bibby Scientific Ltd y ninguna ...

Page 17: ...umo in plastica idonee Dopo aver riscaldato i campioni ricordare che le parti dello strumento cioè il coperchio ed il blocco ed eventuali accessori associati potrebbero essere molto caldi DISIMBALLAGGIO Durante il disimballaggio assicurarsi di aver tolto quanto segue dall imballo Ciclatore termico Cavo di alimentazione di rete Scheda di garanzia Certificato di decontaminazione Si consiglia all ute...

Page 18: ...caldamento diminuirà di circa l 8 agli estremi dell intervallo di tensione 5 Inserire il cavo di rete nella presa che si trova sul retro dello strumento 6 Collocare l unità su un banco piatto idoneo o in una cappa aspirante se necesario assicurandosi che gli sfiati delle prese d aria nella parte inferiore non siano ostruiti Posizionare lo strumento con una distanza minima attorno di 200 mm dalle p...

Page 19: ...chermo del ciclatore termico può essere danneggiato da oggetti appuntiti come penne matite ed unghia Tale tipo di danno sarà considerato uso improprio e renderà nulla la garanzia per questo componente L assistenza secondo quanto stabilito dalla presente garanzia deve essere ottenuta dal fornitore dello strumento Il presente manuale è stato preparato ad uso dei clienti di Bibby Scientific Ltd e nie...

Page 20: ...d Termination Box for the last unit Voltage Selector Select 230V or 115V and ensure correct fuses are fitted Power Lead Connection Ensure that appropriate fuses are fitted for the electricity supply On Off Switch Serial Port Parallel Port RS485 Connections RS 485 RS 232 RS 485 RS 485 TERMINATION BOX DAISY CHAIN P A R A L L E L S E R I A L 1 0 TC512 Techne Manual 19 12 08 3 30 PM Page 20 ...

Page 21: ...ngomi 2433 Nicosia Cyprus Tel 357 22 660427 Fax 357 22 660356 e mail sales techneme com North and South America Techne Inc 3 Terri Lane Suite 10 Burlington NJ 08016 USA Toll free 800 225 9243 Tel 1 609 589 3560 Fax 1 609 589 2571 e mail labproducts techneusa com www techneusa com Italy Bibby Scientific Italia Srl Via Alcide de Gasperi 56 20077 Riozzo di Cerro al Lambro Milano Italia Tel 39 0 2 982...

Page 22: ...e disable Yes Warm up time from ambient 4 minutes Over temperature cut out Yes Regulated heated lid pressure Yes Programs Password protected Yes Incremental decremental hold time Yes Incremental decremental temperature Yes Variable programmable ramp rate steps _ 0 1 C Programmable ramp rate 0 1 C Gradient calculator Yes Maximum programs stored internally 84 Maximum number of segments per loop 60 M...

Page 23: ... Width x Length 260mm x 220mm x 420mm Weight 12 5kg Power consumption 620W including the heated lid Parallel port Yes Serial port RS232 RS485 adaptable Yes Connection to PC Yes 21 TC512 Techne Manual 19 12 08 3 30 PM Page 23 ...

Page 24: ...round a temperature in any step of a program The set temperature is the temperature required in the middle columns of the block and the gradient is the variation at the two extremes of the block the left hand columns being the coolest and the right hand columns the hottest The maximum temperature gradient which can be set is 30ºC the lowest temperature is 20ºC and the hottest temperature is 70ºC E...

Page 25: ...e default lid parameters and those of an individual program can be found on pages 41 and 34 respectively The lid is unlocked by lifting the back of the handle as shown in the diagram below this handle can then be used to lift the lid To close simply lower the lid onto the block containing the samples and press the handle shut The pressure of the heated lid must be adjusted for each consumable type...

Page 26: ...ntrol software to be carried out by either copying software from a new unit to an older one or by obtaining a copy of the software from Bibby Scientific Ltd The memory card can store up to 500 three segment programs the number of programs is reduced as the number of segments in each program is increased to a maximum of 1500 segments in a single program The memory card locates in the slot under the...

Page 27: ...to a typical program At the end of the program measure the volume using a pipette A loss of more than 1 2µl indicates sample evaporation and a vapour leak The volume lost and the change in reagent concentrations determines the minimum volume that can be used Typical volume losses of 1µl in 30 cycles allow the use of samples of 10µl or less PC CONNECTIONS It is possible to connect either a single t...

Page 28: ... be used and neither should abrasive cleaners If the thermal cycler is being used with any radioactive substances the peltier block which is made of aluminum should be cleaned with an agent which is suitable for nonferrous metals such as Neutracon from Decon Labs Ltd However remember that other parts of the unit are made of ferrous materials and may be damaged by spillage onto them Neutracon shoul...

Page 29: ...es in the opposite direction to lock the block into the thermal cycler The software will register and calibrate the unit for the new block It may be necessary to edit the heated lid parameters for a new application for example if the in situ PCR block is fitted for use with hybridisation slides then it is necessary to switch the heated lid OFF as excess heat can cause evaporation of samples Remova...

Page 30: ...GRAMMING THE TC 512 START UP MENU When the TC 512 is first switched on the screen shown below is displayed and will remain until the unit has finished performing a series of self tests This screen displays information such as the serial number of the instrument the type of block being used and the version of software running the thermal cycler Bibby Scientific TC 512 TEST UNIT Performing self test...

Page 31: ...memory card is inserted into the appropriate slot see page 24 Gradient Calculator Calculates the temperature for each column of the block for a user specified temperature and gradient Exit Returns to the start up menu THE SYSTEM USER MENU In this section of the software the user is able to write new programs and edit existing ones Additionally the program defaults for parameters such as the heated...

Page 32: ... amplification by the DNA polymerase in PCR The extra 45 C step is for the reverse transcription and the 94 C step is to denature the reverse transcriptase before the PCR WRITING AND EDITING PROGRAMS There are three options available for creating programs on the TC 512 1 Copy and edit one of the pre written templates 2 Edit a pre written template and save it using a different name 3 Create a new p...

Page 33: ...Exit Select Program 14526 bytes of memory free b 2 STEP PCR TEMPLATE b 3 STEP PCR TEMPLATE b ICE BUCKET b LITIGATION 15 DEGREES b RT PCR TEMPLATE New Exit Program 3 STEP PCR TEMPLATE Last Modified 01 11 1998 00 00 00 Heated lid 105 0 C Heated lid before program On Pause before program Off Initial denaturation 05m00s 94 0 C Hot Start Disabled Number cycles 30 Exit Print Run Edit Delete Copy Enter t...

Page 34: ...details of all the features and parameters see pages 34 Save 32 Select Program 14526 bytes of memory free b ICE BUCKET b LITIGATION 15 DEGREES b RT PCR TEMPLATE i B ACTIN I GAPDH New Exit Program B ACTIN Last Modified 15 05 2006 11 29 17 Heated lid 105 0 C Heated lid before program On Pause before program Off Initial denaturation 05m00s 94 0 C Hot Start Disabled Number cycles 30 Exit Print Run Edi...

Page 35: ...se before program Off Initial denaturation 05m00s 94 0 C Hot Start Disabled Number cycles 30 Exit Print Run Edit Delete Copy Editing 3 STEP PCR TEMPLATE Initial denaturation 05m00s 94 0 C Hot Start Disabled Number cycles 30 Seg MAX C s 00m30s 92 0 C Seg MAX C s 00m30s 55 0 C Seg MAX C s 00m30s 72 0 C Next step Exit Save As PWord Del Ins Enter a new program name There are several text features with...

Page 36: ...iting menu are as follows Del Delete will remove only the highlighted step The default parameters cannot be deleted Ins Insert will add a next step if no Next step is present Save As Allows the program to be saved with an alternative name Save Saves the program PWord Adds a password which protects the program so other users cannot change it Exit This returns the screen to the Select Program menu S...

Page 37: ...y a gradient across the block can be added Number Cycles Up to 99 cycles can be added Inc Dec Allows increasing and decreasing temperatures and times to be set See page 36 for further details Pause Adds a pause as a step User intervention will then be required before the program can move on to the next step INSERTING A SEGMENT Selecting Segment inserts a temperature step of 94 C for 30s at the max...

Page 38: ...eat in the programmed sequence each of the temperature steps for the required number of cycles Edit program function Segment 94 0 C 0 C 00m30s MAX 0 C s Pause Inc Dec 2 Number Cycles 5 Segment OK Cancel Continue 8 0 1 4 7 s m h BS 3 6 9 Edit program function Segment 95 0 C 0 C 00m15s MAX 0 C s Pause Inc Dec 2 Number Cycles 5 Segment OK Cancel Continue 8 0 1 4 7 Max BS 3 6 9 Edit program function N...

Page 39: ...ng large fragments of DNA it is necessary to ensure that the extension is fully complete after each cycle so the extension time is increased with each cycle to compensate for the increased product quantity and decreasing concentrations of reagents The Hold Time of the incremented decremented step is the defined Hold Time plus the summation of the increments decrements The first cycle is never alte...

Page 40: ...NNING A PROGRAM To run any previously saved program simply follow these steps Select Programs from the main System User menu Edit program function PAUSE Pause Inc Dec 2 Number Cycles 5 Segment OK Cancel Continue 8 0 1 4 7 BS 3 6 9 Editing B ACTIN Heated lid 105 0 C Heated lid before program On Pause before program Off Initial denaturation 10m00s 75 0 C Hot Start 95 0 C Next step Final extention 07...

Page 41: ... Stop again If pressing Stop was an error then pressing Continue will enable the program to continue from where it was stopped If Pause was selected then press Unpause to restart the program The pause function might be required for a manual hot start Program 3 STEP PCR TEMPLATE Last Modified 01 11 1998 00 00 00 Heated lid 105 0 C Heated lid before program On Pause before program Off Initial denatu...

Page 42: ...ain and Exit closes the run screen and displays the selected program again Program 100 50 0 00h01m 00h02m 00h03m 00h04m 00h05m B ACTIN Data Log None Cycle 3 of 3 Step 2 of 3 Holding at 55 0 C 10 C Sample Lw 50 0 C Sample Up 60 C Time to complete 01m26s Pause Stop Program B ACTIN Data Log None Exit Run Program 100 50 0 00h01m 00h02m 00h03m 00h04m 00h05m B ACTIN Data Log None Program complete Sample...

Page 43: ... TC 512 TEST UNIT Data Logs Programs Program Defaults Gradient Calculator Exit To access all the parameters it is necessary to move up and down the menu using the cursor arrows The parameters which can be edited are Heated lid Select the Heated Lid and then in the temperature editing window it is possible to enable disable the heated lid If Enabled is pressed the text changes from xxxxx to allow a...

Page 44: ...temperature typically greater than 70ºC after the initial template denaturation The reason is to allow the manual addition of unmodified Taq DNA polymerase which may loose activity if added during the initial 5 minute denaturation Heat activated Taq or Hot Start enzymes do not require this step Final Extension Many PCR experiments require a final step at the end of the cycling phase to allow the p...

Page 45: ...uses only standard characters in the ASCII range 1 to 127 so all common dot matrix pin fed continuous stationery printers can be used For example one printer which could be used is the Epson dot matrix fan form LX300 To access the log files select Data Logs in the main System User menu All of the programs for which a data log has been generated are displayed The cursors can be used to access furth...

Page 46: ...em Version 0 49 1 00 Unit Serial No 88192 01 Last System Test 19 11 1988 09 34 24 Program DEPT PROGRAM Date last edited 12 10 1988 17 46 11 Data log file B ACTIN Event Date Time Func Cycle Step Temp Set point Ramp Rate Program 30 11 98 14 27 26 01 01 Ramping 30 11 98 14 27 26 01 01 01 4 0 C 4 0 C MAX C s Holding 30 11 98 14 27 51 02 01 01 6 3 C 4 C Ramping 30 11 98 14 37 52 02 01 01 4 0 C 95 0 C M...

Page 47: ...en 20 and 70 C Selecting Gradient allows the range of the gradient to be set The minimum is 1 C and the maximum is 30 C An example for the 60 x 0 5ml block is shown The gradient is 10 C with the middle of the block set to 55 C so the block will be approximately 50 C on the left and 60 C on the right and 55 C in the middle of the block It is possible to print the column and gradient information by ...

Page 48: ...CHNE using the touch screen as shown opposite To exit the password screen press Cancel Pressing Enter takes the user through to the system administration menu The System Administration Menu Bibby Scientific TC 512 TEST UNIT Card Utils System Config Exit Sys Admin Password System Test Controller Reset Program Passwords Enter password Q W E R T Y U I O A S D F G H J K L Z Num X C V B N M Enter Cap S...

Page 49: ...lows the user to change the passwords for any password protected program First touch the screen to select the program for which the password needs to be changed and then select Change PWord To change the password simply type the new password by touching the letters numbers and then confirm by pressing Enter It is then necessary to confirm the new password by re typing it SYSTEM ADMINISTRATION PASS...

Page 50: ...l existing data will be deleted from the card Selecting the Erase Card option will erase all the files and programs from the inserted SmartMedia memory card leaving an empty card ready for storing new programs Bibby Scientific TC 512 TEST UNIT RTC OK Exit Bibby Scientific TC 512 TEST UNIT Controller Reset WARNING This will reset the unit to system defaults All stored files will be lost Calibration...

Page 51: ...rtant features are enabling the auto restart in case of a power failure and setting the comms address of the instrument if multiple units are linked to a computer This part of the software also contains information relating to the cycle count for the block and the heated lid The cursor buttons can be used to scroll up and down the menu to access additional parameters A parameter is selected by tou...

Page 52: ...e format from dd mm yyyy to mm dd yyyy Date The date can be corrected by the user by using the numerical interface shown above Time The time can be corrected by the user by using the numerical interface shown in above Auto restart This allows a program to be re started in the event of a power failure when the instrument is in the middle of running a program The options are Enabled or Disabled Baud...

Page 53: ...able a particular temperature step in a protocol to be altered so that the temperature varies across the block By specifying a temperature and the gradient to be applied each column will achieve a different temperature with the set temperature in the middle block the lowest on the left and the highest on the right The gradient is the total difference across the block for example if the set tempera...

Page 54: ...new one of the correct value fuse values are given on the label next to the power inlet Note that fuses should only be replaced by a qualified electrician The holder for the two fuses is built into the mains input socket First remove the power cable and then gently prise the fuse drawer open with a flat bladed screwdriver or similar tool Each fuse can be removed by using the screwdriver as a lever...

Page 55: ...SORIES Product Number Description Quantity FGEN232 Cable and software to connect 1 cycler to PC RS232 only 1 FGEN485D Power pack 230V 485 converter cable and software to connect 2 cyclers to PC 1 FGENONE One cycler cable extension set 1 FGENTWO Two cycler cable set 1 FGENFOUR Four cycler cable set 1 FGENTEN Ten cycler cable set 1 REPLACEMENT PARTS Product Number Description Quantity Required FCABR...

Page 56: ...Via Alcide de Gasperi 56 20077 Riozzo di Cerro al Lambro Milano Italia Tel 39 0 2 98230679 Fax 39 0 2 98230211 e mail marketing bibby scientific it www bibby scientific it Techne Inc 3 Terri Lane Suite 10 Burlington NJ 08016 USA Toll free 800 225 9243 Tel 1 609 589 3560 Fax 1 609 589 2571 email labproducts techneusa com www techneusa com Bibby Scientific Asia Ltd Room 607 Yen Sheng Centre 64 Hoi Y...

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