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6. PRODUCT OPERATION.
6.1. Turbidity Probe calibration.
6.1.1. Open the calibration Screen from the Tools drop down menu.
6.1.2. Adjust the variable in the calibration window. Set the calibration dwell time to 1 minute.
6.1.3. Insert all the probes to be calibrated into a beaker of clear solvent. Make sure there is no air
bubble in the window of each probe.
6.1.4. Begin calibrating by checking the Active box of the probe. Click the calibration button for the
appropriate probe. In the Status bar you will see the temperature ramp to the set value, then
pop-up window will appear to request ‘Load Clear Sample’. Click OK in this window, and the
upper value of transmittance will be calibrated.
6.1.5. Once the calibration is complete, the message ‘Upper Calibration OK’ will appear in the
Status Window. A new pop-up window will appear to request ‘Load Turbid Sample’. Without
taking the probes out of the clear solvent, disconnect the Launch line from the Clarity IR
control box, the click OK in this pop-up window. The lower value of transmittance will be
calibrated. ‘Complete’ will appear in the Status bar at the end of calibration.
6.1.6. If the calibration failed in any of the upper or lower calibration parts, repeat steps 7.1.4 and
7.1.5 until the calibration is completed.
6.1.7. Complete steps 7.1.4 to 7.1.6 for all cells requiring calibration.
6.2. Shoot Through Probe Calibration.
6.2.1. Open Calibration screen from the Tools drop down menu.
6.2.2. Adjust the variable in the calibration window. Set the calibration dwell time to 1 minute.
6.2.3. Insert HPLC vials filled with clear solvent into each shoot-through probe well. Make sure
there is a stir bar in each vial and marking on the HPLC vial will not block the light pathway
of the probe.
6.2.4. Begin calibrating by checking the Active box of the probe. Click the calibrate button of the
probe. In the Status bar, you will see the temperature ramp to set value, then a pop-up
window will appear to request ‘Load Clear Sample’. Click OK in this window and the upper
value of transmittance will be calibrated.
6.2.5. Once the upper calibration is over, the message ‘Upper calibration OK’ will appear in the
Status window. A new pop-up window will appear to request ‘Load Turbidity sample’.
Without changing anything in the HPLC vial, disconnect the Launch line from the Clarity IR
control box, then click OK in this pop-up window. The lower value of transmittance will be
calibrated. ‘Complete’ will appear in the Status bar at he end of calibration.
6.2.6. If the calibration failed in any of the upper or lower calibration parts, repeat steps 7.2.4 and
7.2.5 until the calibration is completed.
6.2.7. Complete steps 7.2.4 to 7.2.6 for all cells requiring calibration. Retrieve the stir bar from the
HPLC vials before disposing of the vials.
Summary of Contents for Electrothermal ATS10232E
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