LB 9510 Lumat
4
Operation with ICE
71450BA2 Rev. 00, 12/2020
Page
35
of
79
[Type of transformation]
select the axis transformation;
availability
of transformation types is dependent on the
selected type of curve fitting.
For spline:
[logit/log], [log/log]
Choose whether to perform a logit/log or
log/log transformation prior to the spline fit.
Use logit/log for standard curves where satu-
ration is expected in the upper RLU range
(usually preferable). You can also select
log/log instead. All standard RLU mean values
Bi and entered concentration values are now
transformed in the logit-log range with usual
normalization of Bi/Bmax.
For linear regression:
[lin/lin], [logit/log],
[log/log]; no smoothing.
For point to point:
lin/lin only (not se-
lectable), no smoothing
For most luminescence immunoassays the
logit/log
transformation
and a
smoothed spline
algorithm work best. For many research as-
says utilizing a standard curve, a
log/log
transformation and
linear
regression
are often used.
[use smoothing]
only available for Spline. If the standard curve
fails to meet certain quality criteria (no bal-
ance point, no or one point of inflection in the
lin/log representation of the values), the
curve is automatically smoothed and the
smoothing factor is displayed. Tic the check-
box if desired.
[Normalization]
Whether
[Normalisation]
will be required will
be indicated by the kit manufacturer. If nor-
malization is turned on, the highest measured
standard
will be set to the RLU value as de-
fined below. The same factor will then be
assigned to all other RLU values. Tic the check-
box if desired.
[RLU]
Define the target RLU value for the highest
standard.
[Low Limit]
Define the low limit for the normalization fac-
tor. If the calculated normalization factor is
under the limit, a warning message will ap-
pear in the report.
[High Limit]
Definethe high limit for the normalization fac-
tor. If the calculated normalization factor is
