D Programming
Benchmark Thermal Cycler User Manual
20
2. In the first screen, edit Temperature Control mode and Lid Temperature
Control mode. You can leave these parameters as default because the default
parameters can meet most PCR reactions.
3. In the second screen, touch and edit parameters of each step. Keep parameters
of a step empty to exclude the specified step. You can touch
Graph
to view
the graphical representation of the program.
4. Touch
Save
to save program or touch
Run
and then select whether save the
program before running according the displayed message in the pop-up
window.
Note:
• Allowable temperature range: 0.0°C to 99.9°C.
• Allowable time range: 00:00 to 99:59 min: sec.
• Allowable number of cycles: 0-101.
• The actual cycle numbers will be equal to the entered cycle numbers. So if
you would perform a PCR reaction with 30 cycles, then you should enter a
number of 30.
