7.
Click on
Camera view
in the navigation bar to see images as they are collected. Cells
outlined in green are counted as live and cell outlined in red are counted as dead.
8.
On the right side of the screen, data is displayed for each image as it is collected and the
overall average is updated as the run progresses. There is also a graph that displays
detailed data from each parameter such as size distribution, % viability per image, etc.
9.
To add additional samples to the queue, click on
Log in Sample
and repeat the process in
Step 4 above. You can only add 9 samples to the queue at any given time. But, you can
continually add samples as carousel positions become available.
10.
When the sample run is complete, the instrument will clean itself. (Note: You cannot
review data images until this process is complete and there are no more samples to be
counted.)
11.
The carousel will then rotate to check for additional samples. At this time, empty sample
cups will be knocked into an internal waster container.
12.
Read general system information below to ensure that you follow proper waste
guidelines.
Analyzing the Sample Run
When the cell analysis is complete, you can flip through the images to check the accuracy of the
instrument in identifying live/dead cells. You will also want to look at the size distribution graph
to ensure that the correct minimum size value was used.
If you would like to change any of the cell type parameters, choose
instrument
from the top tool
bar and scroll down to click on
reanalyze
. At this point, the default cell type window will open
up and you can adjust the necessary parameters and click ok. The cell images will then be
analyzed using the new parameter values and the data will be displayed on the right side of the
screen. Reanalyzing a sample does not overwrite the original data and the new data is not
automatically saved. So you need to choose
save run
from the main menu in the top tool bar to
save data before closing.
If you would like to reanalyze a previous run, then you can reload the data by clicking
open run
from the main menu. Then, select the
text file
from your data folder and the corresponding
images will be automatically loaded as well.
Saving Data
In the user preferences, everyone will have their own directory in which data is automatically
saved. All image files appear as file folders containing tiff files of the individual images and the
data is saved as a text file. If selected during the sample log in process, your data will also be
output to an excel spreadsheet. There is an example excel sheet with explanations in the Vi-Cell
user manual for your reference.
It is recommend that all images are saved during the setup
period so that data can easily be reanalyzed to determine the best cell type parameters.
However, as the hard drive will quickly fill up you will need to alter your preferences at a
later date NOT to automatically save the images.
Please be respectful of how much space you
taking up on the computer and remove any unnecessary files. The computer is not currently
connected to the internet so data will have to be transferred by floppy disk, CD, or USB memory
stick at this time.
