Beckman Coulter SW 32 Ti, Instructions For Use Manual

Page: 9 / 38

LXL-TB-015BB
1
SW 32 Ti and SW 32.1 Ti
Swinging-Bucket Rotors
Specifications for the SW 32 Ti Rotor
1.
Axis of Rotation
Maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32,000 RPM
Density rating at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Relative Centrifugal Field
a
at maximum speed
At r
max
(152.5 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175,000  g
At
r
av
(109.7 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126,000 
g
At r
min
(66.8 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76,600  g
k
factor at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 204
k factor at maximum speed (5 to 20% sucrose gradient; 5°C)
When particle density = 1.3 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 468
When particle density = 1.5 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 428
When particle density = 1.7 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 412
Conditions requiring speed reductions . . . . . . . . . . . . . . . . . . . . see Run Speeds
Number of buckets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Available tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 1
Nominal tube dimensions (largest tube) . . . . . . . . . . . . . . . . . . . . . 25

89 mm
Nominal tube capacity (largest tube) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38.5 mL
Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231 mL
Approximate acceleration time to maximum speed
(rotor fully loaded). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3
/
4
min
Approximate deceleration time from maximum speed
(rotor fully loaded . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1
/
2
min
Weight of fully loaded rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.5 kg (18.7 lb)
Rotor and bucket material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . titanium
a. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed ( rw
2
) to the standard acceleration of
gravity ( g ) according to the following formula: RCF = r  /g — where r is the radius in millimeters,  is the angular velocity in radians per
second (2  RPM /60), and g is the standard acceleration of gravity (9807 mm/s
2
). After substitution: RCF = 1.12r (RPM/1000)
2
r
min
r
av
r
max
1