BD FACSJAZZ User Manual Download Page 9 | Manualshive

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BD FACSJAZZ User Manual Download Page 9

lasted updated by MW on 11/10/2017

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Notes on Data

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Check in your data to protect it

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Data is deleted monthly. If it is not checked in it is gone.

•

USB and personal back up devices are not allowed on our machines.

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Currently we are recommending FlowJo version 10.2

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You will be prompted for Username: ‘flowjo’ Password: ‘314159’.

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Summary of Contents for FACSJAZZ

Page 1: ... users ALWAYS need to bring single stained compensation controls If you have a 4 color panel you should bring 5 compensation controls o One tube stained with each individual antibody o One unstained tube o No PI or viability dye in the single color controls Don t have enough cells for compensation User can purchase compensation beads from many different suppliers eBioscience UltraComp Beads 01 222...

Page 2: ...itude to between 3 and 6 3 Press the ILLUM button 4 Look at the third window of the stream camera 5 Use the left right and the pitch black knobs to adjust the stream so that it is falls into the aspirator and that it is bright and tight like the image below 6 Adjust the stream so that the stream is centered over the pinholes and in focus Using the black left right and the focus adjustment knobs li...

Page 3: ...eft of your stream in the 3rd window of the streams 13 Click FLASH CHARGE and adjust the piezo amplitude till the deflected drop stays out to the left 14 Click SHORT FLASH and adjust the piezo amplitude till the deflected drop stays out to the left like the image below Please note the ILLUM button and the PLATES button Please note the diagram of the nozzle assembly with the labels of each knob Ste...

Page 4: ...running 4 Press ACQUIRE in the acquisition bar 5 Adjust the event rate to about 200events second by adjusting the offset to about 25PSI 6 In the recording settings adjust the events to display to 200 7 Align the beads into their target boxes a Using the color adjustments knobs b Using the focus adjustment knob 8 Press SAMPLE to stop the beads from running And STOP ACQUIRING in the software 9 Remov...

Page 5: ...ss SAMPLE and press BOOST to get them sample running 7 Press ACQUIRE in the acquisition bar 8 Adjust the event rate to between 1000events second and 1500events second by adjusting the offset to about 1 5 PSI 9 In the Sort Layout window make sure the sort device says Accudrop Setup and it is sorting P1 to the left 10 Make sure the sort mode is 1 0 drop pure 11 Click ACCUDROP in the Pressure Console...

Page 6: ...uisition bar to stop acquiring 15 Draw your gates 16 Add your sorting gates to the sort layout 17 Make sure you change the sort mode to 1 0 Drop Pure 18 Load your collection tubes 19 Press SAMPLE to start running your sample 20 Press ACQUIRE in the acquisition bar 21 Press START to sort your sample 22 Choose the number of events you would like to record 23 Press RECORD While your sample is running...

Page 7: ...and clean up procedure 4 Clean up and dispose of anything that came in contact with your samples in the biohazard waste container IF you are the last user of the day continue to the daily shut down IF you are NOT the last user of the day log out of windows and log back into windows as Operator for the next user Daily Shut down Please let staff know when you are done with the cleaning if you are th...

Page 8: ...bbler back under the nozzle with ALCOHOL Lower the nozzle so that it is sitting in the ALCOHOL press PURGE and then PULSE in the software 11 Remove the de bubbler from under the nozzle and dump out any leftover fluid 12 Place a tube of WATER on the instrument and press OVERRIDE button and wait for the water to drip from the nozzle 13 Fill the de bubbler with WATER 14 Place the de bubbler back unde...

Page 9: ... in your data to protect it Data is deleted monthly If it is not checked in it is gone USB and personal back up devices are not allowed on our machines Currently we are recommending FlowJo version 10 2 You will be prompted for Username flowjo Password 314159 ...

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