lasted updated by MW on 11/10/2017
2
Before you begin:
1.
Make sure the plates are clean. You may choose to take them out and clean them.
2.
Close the FACSSortware.
3.
Close the Pressure Controls choosing
Just Quit
4.
Log out of Windows (please make sure you sign out)
5.
Log in to Windows with Your SUNet ID
6.
Open FACS Sortware and push
CONNECT
Stream Start up
*Note: there is an image of the knobs on the at the end of this section to guide you in which knob is which.*
1.
Restore workspace—SSFF template “most recent date”
2.
In the sort settings increase the piezo amplitude to between 3 and 6.
3.
Press the
ILLUM
button.
4.
Look at the third window of the stream camera.
5.
Use the left/right and the pitch black knobs to adjust the stream so that it is falls into the aspirator
and that it is bright and tight.
*like the image below*
6.
Adjust the stream so that the stream is centered over the pinholes and in focus. Using the black
left/right and the focus adjustment knobs.
*like the image below*
7.
You may have to repeat 5 & 6 to get the adjustments correct.
8.
Using the vertical adjustment knob, adjust the nozzle so that it is sitting at the top edge of the panel.
9.
Adjust the piezo amplitude such that there is a proper gap and break of point. This can be monitored
in the second camera view panel. The stream should look similar to this.