lasted updated by MW on 11/10/2017
5
Accudrop Beads
1.
Adjust the piezo amplitude such that there is a proper gap and break of
point. This can be monitored in the third panel. The stream should look
similar to this.
2.
Click on the
PLATES
button to turn on the plates.
3.
Click on
TEST STREAM
in the sort settings window.
(You should see a 2
nd
drop to the left of your stream in the 3
rd
window of the streams.)
4.
Click
FLASH CHARGE
and adjust the piezo amplitude till the second drop
stays out to the left.
5.
Click
SHORT FLASH
and adjust the piezo amplitude till the deflected drop stays out to the left.
*like the image below*
6.
Vortex and load beads on to sample port and press
SAMPLE
and press
BOOST
to get them sample
running.
7.
Press
ACQUIRE
in the acquisition bar.
8.
Adjust the event rate to between 1000events/second and 1500events/second by adjusting the offset
to about 1.5 PSI
9.
In the Sort Layout window, make sure the sort device
says Accudrop Setup, and it is sorting P1 to the left
10.
Make sure the sort mode is
1.0 drop pure
.
11.
Click
ACCUDROP
in the Pressure Console to turn on
the accudrop filter.
12.
Click
START
in the sort layout window.
13.
Adjust the drop delay value until you only see the left
stream visible and the center stream disappears.
*Should look like the images below*
14.
Click
ACCUDROP
in the control window to turn off the accudrop
filter.
15.
Click
STOP
to stop the sort.
16.
Press
SAMPLE
to stop the beads from running.
17.
Remove the beads from the sample port.
18.
Press
BACKFLUSH
, to remove the beads from the sample line.