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Invivo

300

 

 

 

 

 

UM-010 R4.0 

Page 7 of 48 

Invivo

2

 300 User Manual 

 

Regulatory compliance 

 

This product complies with the essential EEA requirements for Electrical Safety and the Low Voltage 
Directive  2006/95/EC  as  well  as  Electromagnetic  compatibility  as  set  out  in  the  EMC  Directive 
204/108/EC. 

 

Symbols 

Before using the Invivo

2

 300, please ensure that you are familiar with the symbols on the Invivo

2

 300. 

Note: The workstation should be located in a well-ventilated area. 

Figure 1: Invivo2 300 symbols 

Symbol 

Meaning 

 

Refer to user manual. 

~

 

Alternating current 

Off 

On 

 

 
Primary earth connection 
 

 

 

This product complies with the essential EEA 
requirements  for  Electrical  Safety  and 
Electromagnetic  compatibility  as  set  out  in 
the EMC directive 2004/108/EC and the Low 
Voltage Directive 2006/95/EC 

 

 

Caution, do not remove covers. No end user 
serviceable parts behind covers. Please refer 
to this manual in all cases where this symbol 
appears, in order to find out the nature of the 
Potential  Hazard  and  actions  to  be  taken  in 
order to avoid the Hazard. 

 

 

 

Summary of Contents for Invivo2 300 UM-010

Page 1: ...Invivo2 300 UM 010 Invivo2 300 User Manual Affix Serial Number Sticker Here...

Page 2: ...transfer 18 plates in 35 seconds Utilised with the ICO2N2IC Advanced Gas Mixing System to offer a microaerophilic environment in which to grow facultative anaerobes Optimum cell environment o Accurat...

Page 3: ...rements 10 Voltage and frequency requirements 10 Power consumption 10 Powering the workstation 11 Workstation overview 12 Workstation layout 12 Component layout 12 Front view 12 Rear view 13 Left side...

Page 4: ...ccess to the main chamber 24 Ezee Sleeve overview 24 Entering the workstation chamber using the Ezee Sleeves 25 Exiting the workstation chamber using the Ezee Sleeves 26 Arm gas displacement volume an...

Page 5: ...38 Replacing the mains plug fuse UK users only 39 Replacing the mains fuses 40 Service requirements 41 Spare parts and accessories 41 Overview 41 Spare parts and cleaning agents 42 Accessories 42 Wor...

Page 6: ...ible is 4 bar gauge The maximum permissible concentration of Hydrogen in the anaerobic mixed gas is 5 5 Hydrogen The mains supply voltage fluctuations must not exceed 10 of the nominal mains voltage O...

Page 7: ...on should be located in a well ventilated area Figure 1 Invivo2 300 symbols Symbol Meaning Refer to user manual Alternating current O Off I On Primary earth connection This product complies with the e...

Page 8: ...ardous components and must not be disposed of at a household waste site Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment Date of...

Page 9: ...External height 650 mm External depth 660 mm Workstation chamber internal width 500 mm Workstation chamber internal height 420 mm Workstation chamber internal depth 460 mm Interlock internal width 15...

Page 10: ...ernal components of the workstation and will invalidate the warranty The gasses are connected to the ICO2N2IC Advanced Gas Mixer See UM 011 ICO2N2IC user manual for more details Electrical supply requ...

Page 11: ...tions inside the room the workstation is located in Powering the workstation To switch the workstation on press the power switch item 2 Figure 14 To switch the workstation off press the power switch i...

Page 12: ...inner and an outer door The Invivo2 300 workstation is controlled via the control panel The ICO2N2IC Gas Mixer controls the gaseous composition of the internal environment See UM 011 for more informa...

Page 13: ...Invivo2 300 UM 010 R4 0 Page 13 of 48 Invivo2 300 User Manual Rear view Figure 5 shows the rear view of the Invivo2 300 workstation Figure 5 Invivo2 300 workstation rear view...

Page 14: ...workstation left side view 1 Pressure relief tank cover pressure relief tank bung 2 Pressure relief tank 3 Pressure relief tank overflow pipe 4 Cable gland optional extra 5 Exhaust valve outlet 6 Vacu...

Page 15: ...vo2 300 workstation with the ultrasonic humidifier Figure 7 Invivo2 300 workstation left side view with ultrasonic humidifier 1 Exhaust valve outlet 2 Cable gland optional extra 3 Humidifier screw cap...

Page 16: ...8 shows the right side view of the Invivo2 300 workstation Figure 8 Invivo2 300 workstation right side view 1 Mixed gas inlet 2 Sample gas return 3 Sample gas out 4 Serial number label 5 Portable Appl...

Page 17: ...switched on 2 Chamber light switch Press to switch the chamber light on off 3 Gas Alarm switch Press to switch the gas alarm on off 4 Humidistat Controls the level of humidity inside the workstation c...

Page 18: ...erature 0 1 C increments or humidity 5 Increase temperature 0 1 C increments or humidity For temperature control the white value PV Process Value is the actual temperature The green value SV Set Value...

Page 19: ...nates to indicate when the interlock inner door is available 2 Inner door button Press to unlock the interlock inner door when available Foot switch control panel Figure 13 shows the foot switch contr...

Page 20: ...Manual Power entry panel Figure 14 shows the power entry panel and label Figure 14 Power entry panel 1 Mains fuse holder 2 On off switch 3 Mains lead connection 4 Nitrogen inlet 5 Primary earth stud...

Page 21: ...of humidity control on Invivo2 workstations Standard Ultrasonic Humidistat is the standard method of humidity control on the Invivo2 300 workstation Ultrasonic is an optional extra Note Ultrasonic hum...

Page 22: ...pening either the outer or inner door Note Both doors should not be opened at the same time Opening the interlock outer door To open the interlock outer door pull the interlock door towards you using...

Page 23: ...the foot switch control panel item 2 Figure 13 The interlock purge cycle takes 30 seconds Access the workstation chamber via the Ezee Sleeves See section 6 2 2 for details When the interlock purge cy...

Page 24: ...into the workstation chamber via the SPES and unloading the interlock Right hand glove port only for unloading the interlock For each method of entry the glove port selector switch Figure 11 must be...

Page 25: ...the Ezee Sleeves Repeat the procedure for the other hand if both hands are to enter the workstation chamber Press and hold the vacuum pedal with your foot item 1 in Figure 13 until the internal gas v...

Page 26: ...e port bar inside the glove port so that it is vertical Tighten the glove port cover knob Do not over tighten the glove port cover knob Supporting the Ezee Sleeve cuff with your opposite hand pull you...

Page 27: ...known as the mailbox SPES overview The SPES consists of an external hinged flap and an internal hinged flap The external flap is held in place by a thumb screw when not in use The internal flap is se...

Page 28: ...back to its closed position Whilst holding the SPES external flap swing the thumb screw right to slot into the SPES external flap Tighten the thumb screw Note Do not over tighten the thumb screw Note...

Page 29: ...tem 3 in Figure 13 To switch the spotlight off remove your foot from the spot pedal on the foot switch control panel item 3 in Figure 13 Note The spot light should not be used continuously for a perio...

Page 30: ...e gland is suitable for cables of diameter 3 5mm to 7mm The cable gland port is located on the left hand side as shown in Figure 6 item 3 Figure 23 shows the cable gland Figure 23 Cable gland To use t...

Page 31: ...ess media from Petri dishes The vacuum port is located on the left hand side of the workstation To use the vacuum port Connect the vacuum source to the external part of the vacuum port by pushing a tu...

Page 32: ...d demonstrations can be found at Ruskinn Technology Limited s YouTube channel http www youtube com ruskinntechnology Video demonstrations are available for Entering and exiting the workstation chamber...

Page 33: ...mer Tristel Fuse Sachets and Duo Foamer are available from Ruskinn Technology Limited see section 7 4 2 for details Ruskinn Technology Limited anti static cleaner Distilled water No other cleaning age...

Page 34: ...e of the SPES o The glove port covers For all workstation components wipe with a paper towel soaked in an appropriate cleaning agent Take care not to get cleaning agents on the fans below the inner re...

Page 35: ...een see UM 011 for more information Either open the SPES see section 6 3 2 or open one of the glove port covers see section 6 2 2 to equalise the pressure between the workstation chamber and the exter...

Page 36: ...ows the O ring removal Figure 28 O ring removal Remove the Ezee Sleeve To install an Ezee Sleeve Place the new Ezee Sleeve onto the glove port ensuring that the O ring grooves are completely covered a...

Page 37: ...e Ezee Sleeves see section 0 for more information Undo the glove port covers and place on the rear shelf Lift the floor tray at the front to expose the detox sachets Figure 29 shows the location of th...

Page 38: ...orage facility Return the ICO2N2IC to the main menu Switch off Invivo2 300 and disconnect from the mains power supply Remove the Ezee Sleeves see section 0 for more information Undo the glove port cov...

Page 39: ...from the mains socket Using a small flat bladed screw driver remove the fuse cover from the mains plug Figure 30 shows the fuse removal Figure 30 Mains plug fuse removal Replace the fuse with a BS 136...

Page 40: ...removal of the mains fuse holder Figure 31 mains fuse holder removal Replace the mains fuses The fuse ratings are o 240V 50Hz F3 15A H250V o 220V 60Hz F3 15A H250V o 110V 60Hz F5A H250V o 100V 50 60Hz...

Page 41: ...ineer Biennial service Biennially 2 yearly Qualified service engineer To arrange an annual or biennial service contact your local distributor Note that the biennial service includes an annual service...

Page 42: ...tion Anaerobic Indicator Strips Anoxic operation Accessories A range of accessories are available to enhance the functionality of your Invivo2 300 workstation Figure 34 is the list of accessories for...

Page 43: ...he workstation is not plugged in The mains socket is not switched on The mains plug fuse has blown The mains fuse has blown Plug the workstation into the mains Switch the mains power on UK users Repla...

Page 44: ...rlock inner door and remove them Wait for the interlock purge cycle to finish Spillages can cause the interlock inner door to stick Clean the interlock inner door See section 7 1 2 7 1 3 for more deta...

Page 45: ...s been left open The workstation has developed a leak Check the Ezee Sleeves are correctly installed See section 0 for more details Check the glove port cover is in place and closed correctly Reclose...

Page 46: ...ARRANTY OF FITNESS FOR A PARTICULAR PURPOSE THE IMPLIED WARRANTY OF MERCHANTABILITY OR ANY IMPLIED WARRANTY ARISING OUT OF A COURSE OF DEALING OR OF PERFORMANCE CUSTOM OR USAGE OF TRADE Ruskinn Techno...

Page 47: ...not be disposed of at a household waste site Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment Alternatively please contact your l...

Page 48: ...les sales ruskinn com Technical support techsupport ruskinn com General enquiries ruskinnoffice ruskinn com Website www ruskinn com YouTube channel http www youtube com ruskinntechnology Ruskinn Techn...

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