ThermoClamp
®
-3 and -3.2
Temperature Controller
User’s Manual
Joe Cordes and David Barton
TM
©
Page 1: ...ThermoClamp 3 and 3 2 Temperature Controller User s Manual Joe Cordes and David Barton TM...
Page 2: ...2021 AutoMate Scientific Inc All rights reserved This equipment must be earth grounded using the post on the back of the controller...
Page 3: ...s and specifications 4 Electronic specifications Acronyms abbreviations and definitions 5 Components 6 Controller unit Perfusion Pencil Perfusion Pencil Placement Bath Perfusion Water Jacket Feature C...
Page 4: ...mware over the USB Port 28 Switching fluids 29 Operating without the bath sensor 30 Maintenance 31 Cutting the Pencil tips 31 Max Temp LED 31 Sensors 32 Replacement Parts 33 Safety Instructions 34 War...
Page 5: ...cations ONLY AutoMate Scientific Inc cannot be responsible for injury or death resulting from medical or pharmacological use Packing List Item Quantity ThermoClamp 3 or 3 2 temperature controller 1 He...
Page 6: ...ondary input for feedback control Perfusion Pencil Inline heater 1 4 or 8 channels for solution heating with flow rates up to 5cc min 37 C Temperature controlled bath perfusion inline heating with a f...
Page 7: ...nitions CU ThermoClamp Controller unit Isopotential Grounding earth point PID Proportional Integral Derivative TC Thermocouple temperature sensor This equipment must be earth grounded using the post o...
Page 8: ...o the Control port is used to control the temperature The thermocouple in Safety is just used to prevent overheating of the Pencil If you only use one sensor connect it to Safety Figure 2 Controller U...
Page 9: ...e Pencil Figure 3 Side view of the Perfusion Pencil Figure 4 Detailed view of the rear part of the Perfusion Pencil Chamber outflow Thermal insulation Multiple solution heater inflow tubes Power cable...
Page 10: ...chamber via submerged tube and ambient temperature and drafts You may be able to mount your Pencil farther from the chamber if you try double tubing use a piece of large Tygon tubing as thermal insul...
Page 11: ...rate chamber or bath outside a perfusion chamber for circulating a heating or cooling liquid The perfusion chamber must be equipped with a water jacket It is not usually something that can be added la...
Page 12: ...and organic solvents To avoid dust contamination we recommend pre rinsing the Perfusion Pencil and tip with distilled water Also it is good practice to discard the first few microliters of solution b...
Page 13: ...CU using the grounding post on the rear face and the Pencil 9 Turn on the on the CU power switch The temperature controller should display the ambient temperature If the Pencil Max Temp LED lights or...
Page 14: ...e from your computer to set the setpoint This input uses 20 deg C V Therefore give the ThermoClamp s BNC input a voltage of 1 6 V for a 32 C setpoint Then you can also use your computer to change it 4...
Page 15: ...ned to the bath around time 575 seconds one can see that its temperature has also risen well over the Setpoint which would have permanently damaged the experiment equipment it is probably undesirable...
Page 16: ...etting ESF or ESB sensitivity to zero ESB runaway ESF stopped flow or by setting the E LO low temperature threshold to its maximum value of 15 0 Figure 8 This second graph shows the temperature contro...
Page 17: ...and heated Pencil can both exist at this temperature indefinitely 10 The controller includes a bi directional USB port on the side It can be used to update the firmware on the ThermoClamp or output a...
Page 18: ...s the input mode for the controller s setpoint AUTO will detect if an analog voltage is connected to the front panel Setpoint BNC connector and use it otherwise it will use the digital setpoint ANLG w...
Page 19: ...s the least sensitive setting with the error enabled Setting the value to 3 results in nominal sensitivity and setting to 4 or 5 will make the error detection more sensitive allowing the controller to...
Page 20: ...ture reaches a value of E LO less than the setpoint and it has been steadily cooling then this error will occur Setting the ELO parameter to a large value decreases its sensitivity since this requires...
Page 21: ...al Port Set the Baud rate and choose the Port probably usbmodem Then click Connect to start watching the USB serial data 2 2 Choose Connection menu Capture to Text Binary File to begin recording the o...
Page 22: ...B This number 0 5 can be changed in the settings menu It determines the sensitivity of runaway error detection Set it to 0 to disable this error detection mode set it to 3 for nominal sensitivity or i...
Page 23: ...el during the brief power on test that measures heater channel sensitivity It will be lower than the maximum current because it is measured when the heater transistors are cool and at their lowest sen...
Page 24: ...ed current higher than the maximum level defined in the settings menu when the safety temperature is toward the lower end of the range and it reduced the software limit in response This can happen nor...
Page 25: ...e bath or that the control sensor may be slowly losing thermal contact with the bath fluid The sensitivity of this error detection mode can be adjusted via the ESF parameter in the settings menu 6 6 C...
Page 26: ...the default No value to Yes and then wait 10 seconds for the settings menu to time out and exit The system will immediately start autotuning when it leaves the settings menu and TUNE has been set to...
Page 27: ...stem Category Response 1 Fast with low overshoot 2 Very fast response 3 Slower without much overshoot 4 Fast with significant overshoot 5 Moderately Slow with significant overshoot 6 Slow or with subs...
Page 28: ...ll not be able to warm the bath sensor above the starting temperature if the safety sensor starts near maximum temperature so the autotuning routine requires that the safety sensor stay below max temp...
Page 29: ...r state is to power cycle the controller turn it off and back on but please check the heater before doing so since there could be something wrong with the way the transistors are mounted or a transist...
Page 30: ...ve Mac or COM port device Windows Ignore the Disk Not Ejected Properly error on Macintosh computers 1 4 Disconnect the USB cable when the transfer has finished 2 If you tried to program the controller...
Page 31: ...bath temperature by increasing heat to the Pencil Without liquid flow none of this added heat reaches the chamber so the Pencil continues heating until the overtemp LED lights or the ThermoClamp dete...
Page 32: ...n your chamber bath temperature or you do not wish to use the ThermoClamp bath thermocouple simply unplug it This way the ThermoClamp will observe and maintain the temperature of the Pencil irrespecti...
Page 33: ...aller 100 m needles This is simply for added rigidity and can be cut if needed Max Temp LED The ThermoClamp is equipped with a warning light to warn you of a high temperature inside the heater If the...
Page 34: ...n infinite resistance measurement Check inside the sensor s blue plug for a loose screw The sensors are simply two different thermocouple wires soldered together Remove any oxidation build up on the p...
Page 35: ...250 m Removable Tip 04 100 100 m Removable Tip 03 08 xxx Heated Perfusion Pencil 8 channel with xxx size tip 03 08 Replacement Heated Perfusion Pencil 8 channel 03 01 j ThermoClamp 3 1 Control Unit 03...
Page 36: ...openings as they may touch dangerous voltage points or short out parts that could result in fire or electric shock Never spill liquids of any kind in the product 5 This product should only be connect...
Page 37: ...been damaged E If the product exhibits a distinct change in performance indicating a need for service STOP If you ever have to remove the main system unit cover observe the following precautions A Th...
Page 38: ...Mate Scientific Inc or its licensor licensees be liable for contingent special direct indirect or consequential damages for the breach of any express or implied warranty or resulting from the use fail...
Page 39: ...AutoMate Scientific Inc 812 Page Street Berkeley CA 94710 U S A 800 998 MATE 6283 international 510 845 6283 fax 510 280 3795 e mail info autom8 com http www autom8 com...
