Astrea Evolve AB070505 Technical User Manual Download Page 40

 

 

PC: Evolve Columns Operating Manual v2 

39 

Two further calculations can be performed to describe column efficiency.  

Reduced plate height - takes into account the particle size of the media and enables comparison 
between different media types. A low value of RPH denotes good column efficiency; a RPH of less 
than 6 is expected. 

(Where Pd is the mean particle diameter) 

(Where the initial sample volume and the values of a and b are all expressed in the same volume 
units) 

 

Note: comparison of results should be undertaken with caution.  

Different media manufacturers often express performance in terms of the definitions above. 
Caution must be taken when interpreting performance results.  

It is advised to check not only the media type used but also the flow rate, column diameter and bed 
height. Often results are given for tests performed in laboratory scale columns. 

 

 

 

Summary of Contents for Evolve AB070505

Page 1: ...Technical User Guide Evolve Process Columns 70 100 140mm diameter columns...

Page 2: ...PC Evolve Columns Operating Manual v2 1...

Page 3: ...rization 12 Handling 12 Chemical compatibility 12 3 Column Installation and Operation 13 Column Installation 13 1 Installation of bed supports 13 2 Installation of Fixed Bed Support 13 3 Levelling the...

Page 4: ...t 30 Connection kit 30 Spares Kit 31 8 Spare Parts Kits Accessories 32 Column Refresh Kit 32 Wetted Flow path Kit 34 Bed Support Kit 34 Elastomer Seal Kit 35 Adjuster Cell Kit 35 Tube Replacement Kits...

Page 5: ...e column with part identification 19 Figure 9 Adjuster Assembly for EvolveTM 100 Process Column 22 Figure 10 Fixed Assembly for EvolveTM 100 Process Column 23 Figure 11 Diagram showing the location of...

Page 6: ...as the following advantages a Ensures the maximum area is available to flow promoting good chromatographic performance and clean ability b Eliminates use of fixings to secure the bed support to the fl...

Page 7: ...4 58 4 58 Operating temperature 2 to 30 C Bed support size 8 m Weights and Dimensions Column 70 100 140 Product Code AB070505 AB100505 AB200505 Adjuster Weight kg 4 5 7 5 Total weight empty kg 11 5 1...

Page 8: ...mn outlet shows the contribution made by the column hardware The tests were performed at ambient temperature 15 20 C using RO water Figure 1 Pressure Drop Evolve 70 mm Process Column Figure 2 Pressure...

Page 9: ...PC Evolve Columns Operating Manual v2 8 Figure 3 Pressure Drop Evolve 140 mm Process Column...

Page 10: ...Flow tube seal fixed and adjuster Santoprene Adjuster bed support seal Santoprene Fixed flow cell Polypropylene black Adjuster flow cell Polypropylene black Anti jet disc 20 glass filled polypropylene...

Page 11: ...wise Concentrations refer to w w All data is referenced to room temperature 15 25 C Chemical Wetted Components Non wetted Stainless Steel 316L 1 2 dichloroethane Acetic acid 25 Acetone 5 Acetonitrile...

Page 12: ...ketone MEK Methylene chloride Nitric acid 10 Nitric acid 70 Peracetic acid 300 ppm Phosphoric acid 55 Potassium hydroxide 2 0 M Iso propanol 20 Iso propanol 40 n propanol 20 Sodium acetate Sodium bica...

Page 13: ...tances that will be used in the column will remain in liquid form during the entire operation at the maximum stated operating temperature In addition The maximum pressure is less than 6 bar The produc...

Page 14: ...eling the column Setting the bed height 1 Installation of bed supports The Evolve Process Column features polypropylene woven mesh bed supports integrated with a Santoprene seal These do not require p...

Page 15: ...cated in the foot ring of the column Place the spirit level on the tube flange and adjust the levelling studs to ensure column is level before proceeding There is approximately 30 mm free vertical mov...

Page 16: ...support 5 Setting the Bed Height Reinstate the adjuster unit in the acrylic tube using the location pins and ensure the adjuster seal is de energized Replace the flange fixing bolts underneath the tub...

Page 17: ...to the correct torque setting See table below 2 Fill the column with water for injection WFI or high quality water and position the adjuster at the operating bed height 3 Ensure the adjuster seal is...

Page 18: ...low cell towards the slurry by turning the height adjust handles but do not enter the slurry mixture 13 The slurry will start to settle when a liquid interface is seen gently lower the adjuster into t...

Page 19: ...ed height has been reached turn the seal actuation knob to apply maximum seal compression 21 After the final compression step flow in the upwards direction to remove any residual air at the top of the...

Page 20: ...PC Evolve Columns Operating Manual v2 19 Figure 8 Schematic of the column with part identification...

Page 21: ...ed that the packed column is stored in 20 ethanol 80 0 1 M NaCl solution at 2 30 C For in process storage the packed column may be stored in any compatible solvent refer to the Chemical Compatibility...

Page 22: ...are not to damage the edge of the flow cells 5 When unpacking columns be careful not to scratch the inside of the acrylic column tubes 6 Be careful not to damage stainless steel surfaces by bumping kn...

Page 23: ...clamp adapter and retain flow tube seal 6 Remove the seal adjust nut and spacer and slide the flow tube and seal adjust tube out of the threaded rod 7 Remove the flow cell locking clip and retain Slid...

Page 24: ...a clean work surface 3 Locate the flats on the process flow tube and using the correct tool remove the flow tube Retain and inspect the flow tube seal 4 To remove the mesh seal assembly from the flow...

Page 25: ...flow tube using correct tools 2 Install the bed support seal assembly on to the flow cell by carefully locating the bed support seal onto the flow cell The bed support should be fitted with the smooth...

Page 26: ...ng Nm Torque Setting Ft lbf 140 mm 20 14 75 100 mm 20 14 75 70 mm 20 14 75 5 Fit the pipework elbow and ensure all connections are secure Note do not over tighten Adjuster Assembly 1 Install the adjus...

Page 27: ...ainless steel central tube and replace the flow cell locking clip Figure 15 Photograph showing the final location of the adjuster bed support 5 Locate the flow tube stainless steel unit inside the thr...

Page 28: ...sanitary clamp adapter to ensure component is correctly tightened 9 Lift the adjuster unit into the acrylic column tube Note alignment pins are located in the top flange to assist orientation CAUTION...

Page 29: ...under cold room conditions or with cold process fluids retighten all bolts If possible empty the column and removed the fixed cell assembly Remove the fixed bed support with integral seal and inspect...

Page 30: ...ecipitate it is possible that the bed supports have become blocked This can also occur if the chromatographic media contains fines If this is suspected replace the bed supports and repeat the pressure...

Page 31: ...open ended 1 X X X Fixed end tool Small 1 X X X Torque wrench 3 8 drive 1 X X X Spirit level 1 X X X Seal tool Micro S driver slotted 1 2 mm 1 X X X Connection kit DESCRIPTION QTY PRODUCT NUMBER AA07...

Page 32: ...support assembly 100 Fixed 1 X Bed support assembly 100 Adjuster 1 X Bed support assembly 140 Fixed 1 X Bed support assembly 140 Adjuster 1 X Adjuster seal Evolve 70 mm 1 X Adjuster seal Evolve 100 mm...

Page 33: ...X Flow Tube Seal 7 0 mm Bore 3 X Adjuster seal 70 mm Column 1 X Adjuster seal 100 mm Column 1 X Adjuster seal 140 mm Column 1 X Pipe spool Elbow 70 mm Column 1 X Pipe spool Elbow 100 mm Column 1 X Pi...

Page 34: ...Fixed Cell 140 mm 1 X Acrylic Tube 70 X 530 mm 1 X Acrylic Tube 100 X 530 mm 1 X Acrylic Tube 140 X 530 mm 1 X Buffer Ring 70 mm 2 X Buffer Ring 100 mm 2 X Buffer Ring 140 mm 2 X Scale Label 70 mm 1...

Page 35: ...w 70 mm Column 1 X Pipespool Elbow 100 mm Column 1 X Pipespool Elbow 140 mm Column 1 X Flow Tube Seal Ladish 3 83 mm Bore 3 X X Flow Tube Seal Ladish 7 0 mm Bore 3 X Bed Support Assembly 70 Fixed 1 X...

Page 36: ...DESCRIPTION QTY PRODUCT NUMBER AA07ACK AA10ACK AA14ACK Adjuster Cell 1 X Adjuster Cell 1 X Adjuster Cell 1 X Flow Cell Locking Clip 70 100 mm 1 X X Flow Cell Locking Clip 140 mm 1 X Anti jet 1 X X X T...

Page 37: ...eter x 1 0 m 2 3 mm Tee 2 Click Clamp 3 4 Ladish 10 Flow Tube Seal Ladish 3 83 mm Bore 10 Blanking Cap 2 Fluid Connection Kit 140 200 250 mm diameter columns DESCRIPTION QTY Product Number 2 Way Sanit...

Page 38: ...DESCRIPTION QTY Product Number Flexible Hose 6 mm Diameter x 2 0 m 2 AAFHK2 Flow Tube Seal Ladish 7 0 mm Bore 2 Click Clamp 3 4 Ladish 2 Torque Wrench Kit 70 100 140 mm diameter columns DESCRIPTION Q...

Page 39: ...ctivity is measured and plotted The plot obtained is then analysed to calculate the following terms of efficiency Chromatographic efficiency is expressed as HETP height equivalent to theoretical plate...

Page 40: ...e Pd is the mean particle diameter Where the initial sample volume and the values of a and b are all expressed in the same volume units Note comparison of results should be undertaken with caution Dif...

Page 41: ...cense from GE Healthcare under US patents 6841097 and 7341202 and any corresponding patents for life sciences research and the purification separation of naturally occurring or recombinant plasma deri...

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