Aquaprobe
®
Lite Instruction Manual
10401-00976 Rev F
Part number: 70301027
Description: Rhodamine WT Liquid
Supplier: Keystone Europe Ltd.
Contact:
Be sure to handle chemicals with care and to read and comply with all health and
safety advice.
11.7.5.
Serial Dilution
The recommended Rhodamine solution is supplied as a 20% or 200g/L solution, dilution of
the stock solution should be carried out as follows.
200g/L stock → 100μg/L is recommended to be done as a two step dilution procedure.
Step 1
; weigh out 0.5g of 200g/L stock solution in a weigh boat and add this to 1L of
deionized water in a volumetric flask, use some of the water from the 1L flask to rinse the
weigh boat so no stock Rhodamine remains on the boat. Put a lid on the 1L flask and
invert 10 times.
This step results in a 1 in 2000 dilution of the stock, at this point the 1L flask will contain a
100mg/L solution.
Step 2
; Transfer 1ml of the 100mg/L solution to a 1L volumetric flask and top up to 1L with
deionized water. Put a lid on the 1L flask and invert 10 times.
This step results in a 1 in 1000 dilution of the solution from step 1. The concentration of
this solution is 100μg/L. This solution can now be used as Pt-2 calibration of the RHOD
sensor.
The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After
that time it must be discarded.
11.7.6.
Zero Point Calibration
To calibrate the zero point, follow these steps:
1. Fill a calibration bottle with mineral water, wash the Probe in mineral water, then
drop the Probe in all the way.
The Sleeve End Cap must be fitted
. Gently agitate
the Probe several times in order to remove any air bubbles that may be clinging to
the electrode.
2. Switch the Aquameter
®
on and wait until the temperature and Rhod readings are
stable. If the Rhod reading is very high, there are probably air bubbles adhering to
the lenses. Agitate the Probe to remove.
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF).
4. Press the
MENU
key then select
Calibration.
The following screen will be displayed.
CALIBRATE Rhod
ZERO? [01/Jan/12]
Pt-2? [01/Jan/12]
© 2015 Aquaread
®
Ltd.
www.aquaread.com
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