3-10
Veriti™ Thermal Cycler User Guide
Chapter 3
Performing a Run
Editing Details
This section describes how to edit a run method by:
• Adding a stage (
)
• Adding a step (
)
• Editing a temperature or a time (
)
• Editing a ramp rate (
)
• Creating an AutoDelta step (
)
• Adding a pause (
• Creating a step to program different temperatures on the VeriFlex Blocks
(
) (Available only on Veriti 96-Well Thermal Cyclers.)
Adding a Stage
The two types of run method stages are:
•
Cycling
– The steps in a cycling stage are repeated a specified number of times.
In general, the steps in a cycling stage are template denaturation, primer
annealing, and primer extension.
•
Holding
– The steps in a holding stage are not repeated.
To add a stage:
1. In the Edit Run Method screen, touch the stage name (at the top of the stage) to
select it. When a stage is selected, the area around the name is red.
2. Touch
Add
.
A copy of the selected stage is added to the run method.
Summary of Contents for Veriti
Page 1: ...Applied Biosystems Veriti Thermal Cycler User Guide ...
Page 2: ......
Page 3: ...Applied Biosystems Veriti Thermal Cycler User Guide ...
Page 8: ...vi Veriti Thermal Cycler User Guide ...
Page 12: ...x Veriti Thermal Cycler User Guide Preface ...
Page 78: ...3 26 Veriti Thermal Cycler User Guide Chapter 3 Performing a Run ...
Page 82: ...4 4 Veriti Thermal Cycler User Guide Chapter 4 Troubleshooting ...
Page 96: ...5 14 Veriti Thermal Cycler User Guide Chapter 5 Utilities ...
Page 106: ...A 4 Veriti Thermal Cycler User Guide Chapter A Instrument Specifications ...
Page 118: ...C 8 Veriti Thermal Cycler User Guide Chapter C Predefined Run Methods ...
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