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Veriti™ Thermal Cycler User Guide
Chapter 3
Performing a Run
Creating an
AutoDelta Step
An AutoDelta step allows you to increase or decrease the temperature and/or change
the duration of the cycle during a cycling stage.
One use for an AutoDelta step is for touchdown PCR, a method in which the earliest
cycles have a high annealing temperature, followed by cycles where the annealing
temperature decreases slightly for each cycle. Touchdown PCR increases the
specificity of the PCR products. The high initial temperature favors more specific
annealing between the primer and the template. Because these fragments are further
amplified during subsequent cycles at lower temperatures, they outcompete the
nonspecific sequences to which the primers bind at lower temperatures.
Note:
AutoDelta steps are allowed only in cycling stages.
To create an AutoDelta step:
1. Touch a step, then
Options
.
The options menu appears:
Summary of Contents for Veriti
Page 1: ...Applied Biosystems Veriti Thermal Cycler User Guide ...
Page 2: ......
Page 3: ...Applied Biosystems Veriti Thermal Cycler User Guide ...
Page 8: ...vi Veriti Thermal Cycler User Guide ...
Page 12: ...x Veriti Thermal Cycler User Guide Preface ...
Page 78: ...3 26 Veriti Thermal Cycler User Guide Chapter 3 Performing a Run ...
Page 82: ...4 4 Veriti Thermal Cycler User Guide Chapter 4 Troubleshooting ...
Page 96: ...5 14 Veriti Thermal Cycler User Guide Chapter 5 Utilities ...
Page 106: ...A 4 Veriti Thermal Cycler User Guide Chapter A Instrument Specifications ...
Page 118: ...C 8 Veriti Thermal Cycler User Guide Chapter C Predefined Run Methods ...
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