Ankom ANKOM200 FIBER ANALYZER, Operator'S Manual

Page: 14 / 38

Operator’s Manual
pg. 14
Rev 1/24/20
6. Tare the weight of the empty filter bag and the holder together.
7. Add 0.45 – 0.50g of sample to the filter bag. Keep all particles away from the sealing area
of the filter bag.
8. Record the weight of the sample (W
2
).
9. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red sealer light
turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of the filter bag. If the
seal is not strong, re-seal the bag.
10. To eliminate sample clumping, spread the sample out uniformly within the filter bag by shaking and flicking the bag.
11. Repeat steps 5 – 10 for all filter bags that will be used in the Analyzer. (Up to 24 bags can be processed during one
procedure with one of the bags being a blank.)
IMPORTANT:
If your samples contain soybean products or >5% fat…
Before doing the ADF analysis in the ANKOM
200
, you will need to do a pre-
extraction. For samples containing non-roasted soybean or >5% fat, follow the
pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times and allow bags to soak for 10 minutes.
5. Pour out and dispose of the acetone.
6. Execute steps 1 through 5 a total of two times.
7. Place the bags on a wire screen to air-dry.
If your samples contain roasted soybean…
Follow the pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times.
5. Pour out and dispose of the acetone.
6. Pour fresh acetone into the container and allow the samples to soak for
twelve hours.
7. Pour out the acetone.
8. Place the bags on a wire screen to air-dry.
12. Place the filter bags with sample and at least one empty bag (used as a Blank) into the Bag
Suspender trays as shown (maximum of three bags per tray).
13. Stack each tray on the Bag Suspender rod (eight trays in total) with each tray rotated 120
degrees from the tray below.
IMPORTANT:
You must use all eight trays even if they are empty.
14. Add the ninth tray to the top of the Bag Suspender rod. This tray contains no filter bags and acts as a cover.
NOTE:
The samples are now ready for the ADF analysis procedure.