Ankom ANKOM200 FIBER ANALYZER, Operator'S Manual

Page: 13 / 38

Operator’s Manual
Rev 1/24/20
pg. 13
ADF Analysis
ADF Calculation
ADF contained within a food or feed sample can be calculated using the following formula:
% ADF (as-received basis) = 100 x (W
3
– (W
1
x C
1
))
W
2
Where: W
1
= Bag tare weight
W
2
= Sample weight
W
3
= Dried weight of filter bag with fiber after extraction process
C
1
= Blank bag correction (running average of final oven-dried weight
divided by original blank bag weight)
ADF Sample Preparation Procedure
To prepare samples for fiber analysis, follow the procedure detailed below.
IMPORTANT:
When using the ANKOM
200
Fiber Analyzer for ADF analysis, at least one
blank filter bag should be included with the sample set as an indicator of
particle loss. A running average of the blank bag weights is used in the fiber
calculation as the C
1
correction factor. A C
1
value larger than 1.0000 indicates
that sample particles were lost from filter bags and deposited on the blank
bag. Any fiber particle loss from the filter bags will generate erroneous
results. If particle loss is observed, the grinding method for the specific
sample should be evaluated.
1. Using a Solvent Resistant Marker, number all of the filter bags you will use during the fiber
analysis.
2. Weigh and record the weight of each empty filter bag (W
1
).
3. Set the Heat Sealer dial to between 4 and 5. (The setting may vary from sealer to sealer.)
4. Seal at least one empty filter bag (to be used as a blank) within 4mm
of its open end. Keep the sealer arm down for 2 – 3 seconds after the
red sealer light turns off (to cool the seal). The seal can be seen as a
solid melted stripe along the top edge of the filter bag (as shown to
the right). If the seal is not strong, re-seal the bag.
5. Place an empty filter bag in the Bag Holder in an open position.
Seal