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B270 User Guide

amscope.com

B270 Specifications

Optical System

Finite conjugate

Mechanical Tube Length

160mm

Head

Binocular, 30° incline, 360° rotation

Interpupillary Adjustment

Siedentopf, 48-75mm

Ocular Diameter

23.2mm

Objective Lenses

DIN standard

Objective Parfocal Distance

45mm

Objective Mounting Thread

RMS 20.32mm

Objective Turret

Forward orientation quadruple

Focusing System

Coaxial coarse and fine focus, upper limit-stop

Fine Focusing Precision

0.002mm

Stage Design

Double-layer with caliper

Stage Vertical Stroke

25mm

Stage Dimensions

118mm x 127mm

X-Y Translation Range

70mm x 21mm

Transmitted Illumination

Variable-intensity 1W LED

Light Condenser

NA1.25 Abbe condenser with iris diaphragm

Sub-stage Condenser-holder

Rack and pinion, centerable

Power

100-240VAC 50/60Hz

What’s In The Box

The B270 standard outfit includes:

•

One microscope

•

Four objective lenses: 4X, 10X, 40X, 100X

•

One pair 10X eyepieces

•

One blue color filter

•

One dust cover

•

One AC power cord

•

25 pipettes

•

50 blank slides with 100 cover slips

•

One security leash with key

Additional items may be included depending on the model.

Summary of Contents for B270

Page 1: ...rtaining to the setup and operation of the B270 biological compound microscope Please familiarize yourself with the necessary precautions and procedures prior to operating this instrument Certain features and specifications are subject to change v0 9 20220624 ...

Page 2: ...environment Electrical components of the instrument can discharge when exposed to water poten tially resulting in damage to the instrument or injury to yourself or others Do not dismantle Dismantling can result in damage to the instrument and potential exposure to dangerous materials or electric current Notices AmScope reserves the right to change specifications of the product at any time without ...

Page 3: ... 1 2 Objective Lenses 9 1 3 Light Condenser 10 1 4 Specimen Stage 11 1 5 Power 12 1 6 Security Leash 12 2 Operation 13 2 1 Mounting a Specimen 13 2 2 Illumination 14 2 3 Using the Condenser 15 2 3a Condenser Height 15 2 3b Adjusting the Aperture 16 2 3c Centering the Condenser 17 2 3d Color Filters 18 2 4 Locating the Specimen and Focusing 19 2 5 Immersion Oil 20 ...

Page 4: ...B270 User Guide 4 amscope com ...

Page 5: ...ision 0 002mm Stage Design Double layer with caliper Stage Vertical Stroke 25mm Stage Dimensions 118mm x 127mm X Y Translation Range 70mm x 21mm Transmitted Illumination Variable intensity 1W LED Light Condenser NA1 25 Abbe condenser with iris diaphragm Sub stage Condenser holder Rack and pinion centerable Power 100 240VAC 50 60Hz What s In The Box The B270 standard outfit includes One microscope ...

Page 6: ...the head become detached simply loosen the thumb screw enough to allow the head to be re seated until flush with the body then tighten the thumb screw CAUTION Never lift the microscope by the head If the head is not properly seated it can become separated causing the body to fall or otherwise damage the coupling mechanism During transport hold the microscope by the body in an upright position to p...

Page 7: ...eyepieces into the ocular tubes Ensure that the eyepieces have matching magnification as some models contain multiple pairs of eyepieces with different magnifications Once attached adjust the diopter ring on the left ocular tube until it is neutrally aligned no dioptric adjustment 1 1c Dioptric Adjustment The dioptric adjuster can be used if your eyes do not focus the same This process requires a ...

Page 8: ...eyepieces with your eyes The adjustment is meant to match the distance between your eyes pupils which is called the interpupillary distance The adjustment process should be performed prior to observation of a specimen and requires that the illuminator is active Attempt to look through both eyepieces adjusting the interpu pillary adjustment mechanism accordingly until the eyepieces are aligned with...

Page 9: ...es are typically mounted in order from 4X to 100X but can be inter changed as desired This microscope is compatible with DIN standard objective lenses which allows aftermarket lenses to be used as needed These lenses must comply with the following DIN specifications 20 32mm RMS mounting thread 160mm mechanical tube length 45mm parfocal distance DIN stands for Deutsches Institut für Normung which i...

Page 10: ...ns must first be re moved Begin by raising the stage using a coarse focus knob to provide more space under the stage Use the condens er holder s height adjuster to raise the holder to its maximum height Unscrew the illuminator s field lens by rotating it counter clockwise until it can be removed Once removed locate the condenser s locking screw on the front of the holder Use one hand to hold the c...

Page 11: ... millimeters from the lens Use the fine focus knob to continue raising the stage until the specimen barely comes in contact with the lens The 100X lens has a spring loaded nose If the nose begins to compress the stage is too high When the specimen is able to make contact with the lens without compressing the nose this is the appropriate maximum height The height adjustment screw should be tight en...

Page 12: ...70 as a theft deterrent The leash should be secured to an immobile object by guiding the leash around or through the object then through the leash s loop Once the leash is secure the lock portion of the leash can be attached to the microscope The microscope s security slot is located on the rear power panel be tween the power switch and power port With the lock in its unlocked position insert the ...

Page 13: ...on 3 x 1 glass slides A metal template with caliper is attached to the top of the stage A prepared slide can be placed on the stage by pushing the caliper lever outward to open the caliper then sliding the slide in place so the long edge is flush against the back edge of the tem plate Gently releasing the caliper will secure the slide in place ...

Page 14: ...roscope s illumination by pressing the power switch to the on position As magnification increases more light will be necessary to view the specimen Use the wheel on the right side of the microscope s base to adjust the illuminator s brightness as needed Avoid using the microscope for long durations with excessive brightness as this can strain the eyes ...

Page 15: ...ust ment knob is located on the left side of the microscope just behind the condenser holder Rotate the knob clockwise to lower the condenser and counter clockwise to raise the condenser Lowering the condenser is typically done to perform a function such as applying immersion oil The height of the condenser also affects its numerical aperture Lowering the condenser will decrease the effective aper...

Page 16: ...ight Decreasing the size of the aperture restricts the amount of light used which in turn reduces the amount of scattered light The notable effects are a reduction in bright ness as well as increased depth of focus Decreasing the aperture too much will result in diffraction artifacts which dete riorate image quality As objective magnification increases more light will be requires to accommodate hi...

Page 17: ...herwise an eyepiece can be removed from the viewing head allowing you to view an image of the iris inside the ocular tube The illuminator must be active to provide the necessary light but the brightness should be adjusted to a low level since you will be looking directly at the light With the condenser raised to its maximum height you should see an image of the iris Use the condenser s adjustment ...

Page 18: ...nsate for light sources with warm er color or to better image certain specimens Yellow filters can be used with cooler light sources like LED to decrease higher intensity wavelengths which can fatigue the eyes over long periods The condenser is equipped with a filter holder located on its bottom The holder has a small peg which can be pulled in a clockwise motion to swing the holder outward The co...

Page 19: ...ith a bright round background If the image appears offset or obscured you should contin ue to adjust the interpupillary distance until the image appears correct The left ocular tube also has a dioptric adjuster which can be used if the eyes do not focus the same If the eyepieces are properly aligned but the light source appears offset use the two centering screws attached to the condenser holder t...

Page 20: ...rture of 1 25 or higher A small drop of oil is placed on the specimen then the lens would be rotated into place The specimen must be appropriately prepared to allow the use of oil such as by using a cover slip When the stage is raised to achieve focus the objective lens will make contact with the oil Immersion oil should also be used with oil compatible condensers Condensers with numerical apertur...

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