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of operation. Rotation clockwise makes tension decrease, and by contraries, rotation 

counterclockwise makes tension increase. 

4.7 Usage of immersion oil objective 

The 100X objective of B series microscope can be used for observation even if 

without immersion oil. However, adding moderate immersion oil between the front 

lens of 100X objective and the cover glass of specimen can make the image clearer. 

Please pay attention that air bubble and impurity can’t be in the immersion oil, 

otherwise, the image would be affected. 

First of all, take 40X objective which has been focused well out from bright path, 

then take 100X objective into bright path. At this time, nosepiece or stage moving 

knob should be turned slightly, and also slightly turn fine focusing knob to clear away 

the air bubble of immersion oil, otherwise, the air bubble would affect the image 

badly. 

After immersion oil used, the oil of specimen and the microscope surface should be 

soon cleaned by absorbent cotton, lens paper, gauze or soft cotton cloth with 

moderate mixture of pure industrial alcohol and ether (proportion 1

:

4) 

Standard thickness 0.17mm cover glass should be chosen when high times objective 

used, and thickness error should be within 0.01mm, otherwise, image definition 

would be affected. 

4.8 Mirror (Shown in Fig.4) 

Mirror of B series microscope is optional, and it is used when electrical power isn’t 

supplied. 

Mirror doesn’t be suggested as illumination source when 100X objective used.   

Summary of Contents for B100

Page 1: ...15 B100 B200 Series Biological Microscope Operation Instruction To ensure the safety and obtain satisfactory performance please study this operation instruction thoroughly before your operation ...

Page 2: ...16 Contents 1 Purpose 2 2 Specifications 2 3 Installation 4 4 Operation 4 5 Maintenance 8 6 Troubleshooting 10 7 Outfits 13 ...

Page 3: ... Handleof IrisAperture Pow er S witchAnd Light Collector Fig 1 B200Binocular Biological Microscope FineFocusingKnob Light Controller Condenser Up downKnob Coaxial CoarseAnd ElectricBase Eyepiece Nosepiece O bjective FineFocusingKnob Monocular Head MainBody Clamp S tage Condenser Light Controller Power S witchAnd Light Controller Fig 2 B100Monocular Biological Microscope ...

Page 4: ...d family 2 Specifications 2 1 Total magnifications Objective Total Magnifications Eyepiece 4X 10X 40X 100X 10X 40X 100X 400X 1000X 16X 64X 160X 640X 1600X 2 2 Objectives Objective Numerical Aperture N A Objective Field Resolving Power Working Distance Field Number ф18 Field Number ф20 Achromatic Objective Plan Objective 4X 0 10 4 5mm 5mm 2 8µm 13mm 16mm 10X 0 25 1 8mm 2mm 1 1µm 6 3mm 2mm 40X S 0 6...

Page 5: ... Mechanical stage Size 142mm 134mm X Y travel 50mm 70mm 2 3 6 Focusing systems Coaxial coarse and fine focusing knobs Coarse stroke 22mm Fine division 2µm Condenser up down range 25mm 2 3 7 Condenser Abbe condenser N A 1 25 Adjustable aperture Aperture center is adjustable 2 3 8 Mirror Mirror diameterф52mm optional 2 3 9 Filter Built in blue filer ф38 5mm 2 3 10 Anti fungal systems Eyepiece and ob...

Page 6: ...king box and drive them into the holes of nosepiece orderly and tightly according to the times Take out the oil bottle and dust cover and place it properly for convenient use 4 Operation 4 1 Electric components Make sure the supply voltage meets the instrument s requirement then plug in it Rotation potentiometer with power switch used by the instrument is for protecting lamp Mirror Bracket Mirror ...

Page 7: ...st size of iris aperture for adjusting the contrast of the specimen 4 4 Interpupillary distance and diopter adjustment Shown in Fig 5 4 4 1 Interpupillar distance is different for everyone so interpupillary distance should be adjusted before using binocular microscope Please take the two eyepiece tubes to turn until the bright rings observed by two eyes are in superposition entirely B100 monocular...

Page 8: ...n t turn left and right coarse and fine focusing knobs with different direction with power at the same time if so the focusing system will be damaged Don t directly pull objective to turn nosepiece when user changes the different objectives if so optical quality of microscope possibly be affected The right way is to take the tooth like part of the nosepiece to turn it and make the objective into c...

Page 9: ...nto bright path At this time nosepiece or stage moving knob should be turned slightly and also slightly turn fine focusing knob to clear away the air bubble of immersion oil otherwise the air bubble would affect the image badly After immersion oil used the oil of specimen and the microscope surface should be soon cleaned by absorbent cotton lens paper gauze or soft cotton cloth with moderate mixtu...

Page 10: ...on Finally cover microscope with dust cover 4 9 2 Please take eyepiece and objectives out from microscope if user will stop using it for a long time and place eyepiece and objectives into drier with drying agent Cover microscope with dust cover 5 Maintenance 5 1 Clean microscope 5 1 1 Don t touch the lens with hand Dust on lens should be cleaned by soft brush or absorbent cotton or cleaned by abso...

Page 11: ...s and mist damage instrument 5 2 4 Please pay attention to prevent microscope from violent shake and vibration in application and in carrying Don t drag it on the surface of worktable to avoid damage microscope and worktable U screwKnurled n Lam pAdjustm ent Knurled Pull O ver Bulb HousingC over ReplaceBulb C loseC over TheLam p C over Thum bS crew Thum bS crew Fig 7 Replacement of Bulb 5 3 Replac...

Page 12: ...ly better cut off the microscope about 30 minutes 5 4 Replacement of fuse Shown in Fig 8 5 4 1 Cut off power of microscope and pull out the plug 5 4 2 Unscrew fuse cap in the back of base take out old fuse 5 4 3 Replace a new fuse then screw the fuse cap 5 5 Stop to use microscope please cut off power cover the dust cover and place it in a cool and dry environment 6 Troubleshooting In the period o...

Page 13: ...uirement Replacing bulb Brightness isn t adjusted correctly Adjust rotation potentiometer Objective isn t in correct position Make the objective in correct position The size of iris aperture is too small Adjust the size of iris aperture Lens objective eyepiece condenser light collector has dust Clean it Position of condenser is too low Higher condenser Image isn t clear Cover glass of specimen doe...

Page 14: ... of image is dark or image is moving as focusing Objective isn t in correct position Make the objective in correct position Specimen isn t placed correctly Place specimen levelly on stage and clip it with clamp Objective touches specimen as changing low times objective to high times objective Cover glass of specimen isn t in up direction Place specimen correctly Cover glass doesn t meet the requir...

Page 15: ...10 B A4 10X 0 25 B A10 40X 0 65 S B A40 100X 1 25 Oil S B AO100 Plan Objective 4X 0 10 B P4 10X 0 25 B P10 40X 0 65 S B P40 100X 1 25 Oil S B PO100 Monocular Head Inclined 30 Rotatable 360 Mo 1 Seidentopf Binocular Head Inclined 30 Rotatable 360 Interpupilary Distance 55 75mm Bi 1 Nosepiece Quadplex Coaxial Coarse and Fine Focusing Knobs N 4 Fixed Stage Clips S 1 Attachable Mechanical Stage Travel...

Page 16: ...lt In Blue Filter Mo Incandescent Lamp 110V 220V 20W Spare Lamp 1pc Built In Blue Filter E1 Halogen Lamp 6V 20W Wide Voltage 85V 265V Spare Lamp 1 pc Built In Blue Filter E2 LED Illumination Systems LED Fuse Spare Fuse 2 pcs Note In Table Is Standard Attachment Is Optional Accessories Packing Size 340mm X 243mm X 425mm Gross Weight 7 kgs Net Weight 5 5 kgs ...

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