Amersham Pharmacia Biotech EPS 3501 XL, User Manual

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elapsed time, volthours or milliamperehours by
Ô
/
Õ
. The parameter controlling the electrophoresis is
underlined.
A light emitting diode shows when voltage is applied (HV on).
If no current is displayed or if “HALT: Low start current” is shown, please check the electrical
connections to the electrophoresis equipment.
Pausing
You can interrupt the electrophoresis for sample loading and/or changing the program by pressing
PAUSE CONTINUE. Voltage will no longer be supplied, the “HV on” LED goes off, and you may safely
load your samples. The display shows the status of the run when PAUSE CONTINUE was pressed.
Switch between time, integrated voltage and integrated current for the phase running when PAUSE
CONTINUE was pressed with
Ô
/
Õ
.
When sample loading is complete, press either PAUSE CONTINUE again or RUN to continue the run
from where it was interrupted.
Editing a running program when in PAUSE
When in the PAUSE mode you can also press SET ENTER to make changes in the program. This mode is
called the P-SET mode. When the P-SET mode is entered you can make changes as described in the
SET: 2 2 1000 Vh
2000 V 20 mA 15 W
SET: 2 2 00:00 h
0 V 0 mA 0 W
Fig. 6. Inserting and deleting a phase in a program.
Insert Phase 2
INS/del
SET: 2 1 1000 Vh
1000 V 120 mA 10 W
SET: 2 1
step/GRADIENT
SET: 1 1
A
1 Vh
500 V 1 mA 5 W
1. Choose Program Number (1
→
2)
2. Confirm Step or Gradient.
(Step)
3. Choose Phase number (2) and
enter INSERT/DELETE
4. Choose INSERT or DELETE
Programming point 4
5. Program
a new
phase 2.
INSERT DELETE
5. Back to new
Phase 2 start
position.
Old Phase 2
deleted.
enter
set
Select by Confirm by
enter
set
Confirm by
Insert
delete
Select by Enter by
enter
set
Select by Confirm by
EPS 3501 XL - User Manual
18-1130-22 AA EPS 3500XL inlaga 99-02-10 13.09 Sidan 12