Amersham Biosciences MegaBACE 1000, Planning Manual, Page: 28 / 50

3.2 Chemistry
MegaBACE 500 and 1000 Planning Guide
●
p3-5
Table 3-7.
Chemistry requirements for STR genotyping
Item Storage conditions Quantity Supplier Part number
*
* You can order the reagents from your local Amersham Biosciences sales office. For a complete listing, see the Web site: http://www.amershambiosciences.com.
MegaBACE long-read matrix
(LPA) and buffer 4 °C 96 tubes per pack Amersham Biosciences
http://www.amershambiosciences.com US79676
MegaBACE energy transfer (ET)
size standard 4 °C (reduced
exposure to light) Amersham Biosciences
http://www.amershambiosciences.com
• ET400-R 500 µl
2.5 ml 25-0205-01
25-0205-02
• ET550-R 500 µl
2.5 ml 25-6550-01
25-6550-02
• ET900-R 500 µl
2.5 ml 25-6900-01
25-6900-02
MegaBACE loading solution
(70% formamide, 1mM EDTA)
−
20 °C 40 ml Amersham Biosciences
http://www.amershambiosciences.com US79916
Spectral matrix standards; one
for each dye in your dye set Use the supplier who provides
your primers.
MegaBACE genotyping
test plate kit
−
20 °C 2 plates Amersham Biosciences
http://www.amershambiosciences.com 25-0207-01
Table 3-8.
Chemistry requirements for SNP genotyping
Item Storage conditions Quantity Supplier Part number
*
* You can order the reagents from your local Amersham Biosciences sales office. For a complete listing, see the Web site: http://www.amershambiosciences.com.
MegaBACE long-read matrix
(LPA) and buffer 4 °C 96 tubes per pack Amersham Biosciences
http://www.amershambiosciences.com US79676
10
×
MegaBACE LPA buffer 4 °C 50 ml
1 liter Amersham Biosciences
http://www.amershambiosciences.com US79677
US79686
MegaBACE loading solution
(70% formamide, 1mM EDTA)
−
20 °C 40 ml Amersham Biosciences
http://www.amershambiosciences.com US79916
MegaBACE SNuPe genotyping kit
(including premix, control primer,
control template, and spectral
matrix standards)
−
20 °C 1 000-reaction pack
10 000-reaction pack Amersham Biosciences
http://www.amershambiosciences.com 25-6001-01
25-6001-02
MegaBACE SNuPe
multiple-injection marker kit
−
20 °C 1 000-reaction pack
10 000-reaction pack Amersham Biosciences
http://www.amershambiosciences.com 25-6001-03
25-6001-04
MegaBACE SNuPe control kit –20 °C 4
×
10 reactions Amersham Biosciences
http://www.amershambiosciences.com 25-6001-05
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