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Amersham Biosciences MegaBACE 1000 Planning Manual Download Page 25

Chapter 3   Emission filters, chemistry, and laboratory materials

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MegaBACE 500 and 1000 Planning Guide

Table 3-1.   

Beamsplitters and filters for the DYEnamic™ ET terminators

DYEnamic 
ET terminator dye

DYEnamic 
ET terminator 
base order

Spectral
channel

Filter

Beamsplitter

ET-R6G

T

1

555DF20

A: 540DRLP

ET-R110

G

2

520DF20

ET-ROX

C

3

610LP

B: 595DRLP

ET-TAMRA

A

4

585DF20

Table 3-2.   

Beamsplitters and filters for the DYEnamic ET primers

ET primer dye

ET primer 
base order

Spectral 
channel

Filter

Beamsplitter

ET-R6G

A

1

555DF20

A: 540DRLP

ET-R110

C

2

520DF20

ET-ROX

T

3

610LP

B: 595DRLP

ET-TAMRA

G

4

585DF20

Table 3-3.   

Beamsplitters and filters for the SNuPe dye set

SNuPe dye

SNuPe 
base order

Spectral 
channel

Filter

Beamsplitter

R6G

A

1

555DF20

A: 540DRLP

R110

G

2

520DF20

ROX

C

3

610LP

B: 595DRLP

TAMRA

T

4

585DF20

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Summary of Contents for MegaBACE 1000

Page 1: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

Page 2: ...MegaBACE 500 and 1000 Planning Guide Revision 6 December 2002 user manual 256 003 63004918 SV 399 326 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Page 3: ...lecular Systems and F Hoffmann La Roche Ltd A license to use the PCR process for certain research and development activities accompanies the purchase of certain reagents from licensed suppliers such a...

Page 4: ...e license arrangements from PE Applied Biosystems The above patent rights are granted solely for research and other uses that are not unlawful No other licenses are granted expressly impliedly or by e...

Page 5: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Page 6: ...production 2 4 2 3 System electrical requirements 2 4 2 3 1 Uninterruptible power supply UPS 2 5 2 3 2 Instrument and power supply fan module North America and Japan 2 5 2 3 3 Instrument and power su...

Page 7: ...apter 4 Planning for computer network and data storage 4 1 Basic MegaBACE system configuration 4 1 4 2 Configuration for a high throughput laboratory 4 2 4 3 Storage requirements 4 2 Chapter 5 Moving...

Page 8: ...es for the instrument MegaBACE instrument administrator s guide provides information on how to tailor the instrument control software for your laboratory and applications MegaBACE analysis software us...

Page 9: ...Netherlands Tel 01 576 0616 10 Tel 0165 580 410 Fax 01 576 0616 27 Fax 0165 580 401 Belgium Norway Tel 0800 73 888 Tel 2318 5800 Fax 03 272 1637 Fax 2318 6800 Canada Portugal Tel 1 800 463 5800 Tel 2...

Page 10: ...lows you to perform detailed analysis of the data This chapter provides a summary of Preinstallation tasks section 1 1 MegaBACE system components section 1 2 Additional recommended components section...

Page 11: ...checklist 1 2 MegaBACE system components The MegaBACE system consists of integrated hardware figure 1 1 software reagents chapter 3 and capillary arrays The basic MegaBACE system components are MegaBA...

Page 12: ...line fluctuations See section 2 3 1 for details 1 4 Network The MegaBACE instrument and the workstation are connected using a computer network Instrument connection PCI peripheral component interconn...

Page 13: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Page 14: ...need to plan the space accordingly See chapter 4 for workstation configurations 2 1 1 Instrument space requirements The dimensions of the MegaBACE instrument are Width 1 033 m 40 7 in Height 0 812 m...

Page 15: ...nce for chamber door 0 914 m 36 in 1 160 m 45 7 in Laser exhaust port 1 524 mm 60 in 1 219 m 48 in width by 0 914 m 36 in depth minimum Notes 1 Allow 0 127 m 5 in clearance behind the instrument for s...

Page 16: ...se You can order the customized table product number 94 9900 28 from the following site Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Postfach 5480 D 790 21 Freiburg Germany 49...

Page 17: ...You can choose to vent the hot air out the back of the power supply fan module into a duct To do this requires a 0 102 m 4 in flange Contact MegaBACE System Technical Support for details The airflow...

Page 18: ...Note A UPS will most likely require electrical connections at the wall that differ from those of the MegaBACE instrument Contact the UPS manufacturer or MegaBACE System Technical Support for details...

Page 19: ...lease refer to the manufacturer s guidelines and follow all applicable local electrical codes during the site preparation Contact the UPS manufacturer or MegaBACE System Technical Support for more inf...

Page 20: ...figuration A single nitrogen source for both high and low pressure supplies High pressure for injecting and removing the matrix Low pressure for rinsing the capillaries and operating the cathode and a...

Page 21: ...ring information for the manifold assemblies Option 2 Ordering separate parts If you are using multiple instruments contact MegaBACE System Technical Support for assistance See Assistance in the prefa...

Page 22: ...ay 0 003 m3 0 1 ft3 Table 2 5 Ordering information for nitrogen systems outside the US Item Description Suggested supplier company name telephone number Product or Code number Europe N2 Panel for Mega...

Page 23: ...itrogen pressure To prevent pressure loss during a cycle you should check the available nitrogen pressure on a regular basis to determine when to replace the cylinders 2 4 6 Installing a nitrogen cyli...

Page 24: ...rious applications The topics are Emission beamsplitters and filters section 3 1 Chemistry section 3 2 Laboratory equipment section 3 3 Consumables section 3 4 3 1 Emission beamsplitters and filters 3...

Page 25: ...TAMRA A 4 585DF20 Table 3 2 Beamsplitters and filters for the DYEnamic ET primers ET primer dye ET primer base order Spectral channel Filter Beamsplitter ET R6G A 1 555DF20 A 540DRLP ET R110 C 2 520DF...

Page 26: ...notyping filter set 2 Dye set 2 Alternatives for dye set 2 are ET ROX FAM TAMRA and HEX or ET ROX FAM TAMRA and JOE Color Spectral channel Filter Beamsplitter ET ROX Red 1 610DF20 A 540DRLP FAM Blue 2...

Page 27: ...rimer Set 5 ATT TAG GTG ACA CTA TAG 3 20 C 2000 reactions per pack US79850 T7 ET Primer Set 5 TAA TAC GAC TCA CTA TAG GG 3 20 C 2000 reactions per pack US79855 21 M13 Forward ET Primer Set 5 TGT AAA A...

Page 28: ...207 01 Table 3 8 Chemistry requirements for SNP genotyping Item Storage conditions Quantity Supplier Part number You can order the reagents from your local Amersham Biosciences sales office For a comp...

Page 29: ...ks Pipettes with volumes of 5 ml 10 ml and 25 ml Heat block or 96 well thermal cycler able to maintain 95 C Squirt water bottle 20 C storage for the samples and reagents 4 C storage for the linear pol...

Page 30: ...wrsp com Within US 1 800 932 5000 Outside US 908 465 4045 or refer to Web site 20170 217 Capillary arrays 16 capillary arrays Amersham Biosciences see the preface 63 0008 84 Capillary window cleaners...

Page 31: ...om contact html Within US 1 800 752 8585 Outside US 408 734 8500 or refer to Web site 1055 00 X 10 plates standard 1055 01 X 10 plates sterile 1055 90 X 50 plates Note X designates a number for plates...

Page 32: ...035 89 Be sure to verify that you order the correct type of plate for your instrument s cathode assembly The skirted plate version of the cathode assembly is convertible to the Robbins plate design If...

Page 33: ...mables for cleanup of the SNuPe reaction Item Quantity Supplier Product code AutoSeq 96 dye terminator cleanup kit 10 pack 100 pack Amersham Biosciences see http www amershambiosciences com for site l...

Page 34: ...t information 4 1 Basic MegaBACE system configuration The basic configuration for the MegaBACE system includes one MegaBACE instrument and one workstation figure 4 1 A PCI SCSI connects the two compon...

Page 35: ...ation If you plan to have an additional analysis workstation that is not in the same room with the instrument control workstation make sure you have the proper network drops for the computer connectio...

Page 36: ...nt Warning The MegaBACE instrument weighs approximately 272 kg 600 lb The instrument requires adequate physical support Never attempt to lift the instrument without using proper equipment and trained...

Page 37: ...ninterruptible power supply UPS is recommended to reduce the instrument s vulnerability to power line fluctuations 5 3 Connecting a new computer To connect your MegaBACE instrument to a new computer f...

Page 38: ...the Amersham Biosciences MegaBACE representative will need this information to schedule your MegaBACE system installation Organization name User manager name s Telephone Email Address Additional stor...

Page 39: ...ptacles appropriate for your country sections 2 3 2 and 2 3 3 Provide a phone line in the lab recommended for talking to MegaBACE Technical Support Laboratory Provide a laboratory bench rated to hold...

Page 40: ...rmal cycler Microplates 96 well Plate sealer Capillary arrays Deionized filtered water Squirt water bottle Ice bucket Wet ice Assorted beakers and flasks Uninterruptible power supply optional but reco...

Page 41: ...d matrix LPA MegaBACE sequencing standards M13mp18 strand DNA pUC18 pUC19 STR genotyping chemistry See section 3 2 for ordering information MegaBACE long read matrix LPA and buffer MegaBACE loading so...

Page 42: ...cols and procedures Instrument hardware components System startup and shutdown Workflow Capillary array installation and replacement Changing optical filters System software System startup Setting the...

Page 43: ...intenance and troubleshooting System specifications Cleaning the cathode and anode Cleaning the air filter Cleaning the water tanks and bottles Check if completed Tasks and subtasks Artisan Technology...

Page 44: ...can be located below the laboratory bench The UPS if used can be located on the floor near the laboratory bench 0 279 m 11 in 0 563 m 21 in 0 393 m 15 5 in Instrument control workstation Computer and...

Page 45: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Page 46: ...2 C 1 weight 2 3 configurations dye set and emission 3 3 cylinders see nitrogen D data transfer and storage 4 2 dimensions computer 2 2 instrument 2 1 laboratory bench 2 2 power supply fan module 2 2...

Page 47: ...2 5 1 nitrogen cylinder 2 10 instrument airflow requirement 2 4 clearance above 2 1 dimensions 2 1 2 2 electrical requirements 2 4 to 2 6 moving 5 1 space requirements 2 1 2 2 C 1 weight 2 3 instrumen...

Page 48: ...trument 5 1 S SCSI connection 1 3 sequencing chemistry requirements 3 4 consumables 3 7 dyes and emission optics 3 1 laboratory equipment 3 6 site information form A 1 site requirements 2 1 to 2 10 5...

Page 49: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Page 50: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

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