Operation
3
Pump P-960
User Manual 18-1172-73 Edition AA
15
4
Purge the pump before use as described in section 5.2 Removing
trapped air bubbles from the sample pump or in ÄKTAdesign
Optional Configurations User Manual.
5
Set the pressure limit as described in section 3.4 Setting the pressure
limit. If the pressure limit is exceeded, the pump is paused.
Note: When applying the sample directly onto the column, some
columns generate a back-pressure above 2.0 MPa (the upper
pressure limit of Pump P-960) at their recommended flow
rates. To maintain a back-pressure below 2.0 MPa when
using these columns, we recommend decreasing the flow
rate.
Note: We recommend decreasing the flow rate of the system pump
during equilibration, before sample application, and before
switching the sample pump in-line. The sample pump will
not start if the back-pressure exceeds 2.0 MPa.
3.3.2
Emergency stop
In case of emergency, switch off the power to the ÄKTAdesign system.
3.3.3
Setting the flow rate, starting and stopping the pump
To set the flow rate and start the pump manually:
1
In the
System Control
module, select
Manual:Pump
.
2
Select the instruction
SampleFlow_960
in the list.
3
Type the desired flow rate.
4
Click on the
Execute
button. The pump starts.
5
To stop the pump, set the flow rate to 0 and click on
Execute
.
The pump can be run without liquid, but this should be avoided since
it might increase the mechanical wear.
18117273aa.book Page 15 Thursday, July 3, 2003 5:13 PM