3
Operation
16
Pump P-960
User Manual 18-1172-73 Edition AA
3.4
Setting the pressure limit
When applying the sample directly onto the column, it is important to
set the upper pressure limit of the sample pump according to the
pressure specification of the column that is used.
To set the upper pressure limit:
1
In
System Control
, select
Manual:Alarm&Mon
.
2
Select the
Alarm_SamplePressure_P960
in the instruction list.
3
Select the
Enabled
mode.
4
Type the desired pressure limit in the
HighAlarm
field.
5
Click on the
Execute
button.
3.5
Calibration
The sample pump is calibrated at the factory.
If pressure pulsation or flow inaccuracy occur, first remove any trapped
air from the sample flow path as described in section 5.2 Removing
trapped air bubbles from the sample pump. Alternatively, follow the
instruction for your configuration in the ÄKTAdesign Optional
Configurations User Manual.
If the flow rate remains out of specification, contact the local
Amersham Biosciences service representative.
3.5.1
Calibrating the pressure offset
To calibrate the pressure offset:
1
Expose the pressure sensor to atmospheric pressure only.
2
In the
System Control
module in UNICORN, select
System:Calibrate
.
3
Select
P960Press
.
4
Click on the
Start Calibrate
button.
The system adjusts the pressure offset of the pump.
18117273aa.book Page 16 Thursday, July 3, 2003 5:13 PM