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Amersham Biosciences AKTA design P-960 User Manual Download Page 6

6

Pump P-960

 User Manual 18-1172-73 Edition AA

Short instructions on back page

About this manual

This manual comprises two parts; a practical part (sections 1–5) and a 
reference part (section 6). Sections 1–5 contain the necessary 
information for installing, operating and maintaining the instrument.

18117273aa.book  Page 6  Thursday, July 3, 2003  5:13 PM

Summary of Contents for AKTA design P-960

Page 1: ...Pump P 960 18 1172 73 User Manual 18117273aa book Page 1 Thursday July 3 2003 5 13 PM...

Page 2: ...18117273aa book Page 2 Thursday July 3 2003 5 13 PM...

Page 3: ...G This is a Class A product In a domestic environment this product might cause radio interference in which case the user might be required to take adequate measures Terms and Conditions of Sale Unless...

Page 4: ...18117273aa book Page 4 Thursday July 3 2003 5 13 PM...

Page 5: ...he pressure limit 16 3 5 Calibration 16 3 6 Filling the sample inlet tubings 17 3 7 Ending the run and storage 18 3 8 Changing liquid 18 3 9 Restart after power failure 18 4 Maintenance 4 1 Periodic m...

Page 6: ...k page About this manual This manual comprises two parts a practical part sections 1 5 and a reference part section 6 Sections 1 5 contain the necessary information for installing operating and mainta...

Page 7: ...ms it is used as a sample pump Pump P 960 features Operating pressure up to 2 0 MPa 20 bar 290 psi Flow rates up to 50 ml min A pressure sensor connected to the pump outlet Interface for connecting an...

Page 8: ...bing might loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are being used Connect the tubing by first inserting the tubing fully then tightening the con...

Page 9: ...e located in a place of low temperature variations away from heat sources draught and direct sunlight The unit may be operated at normal ambient temperature in the range 4 C to 40 C 2 3 Installing the...

Page 10: ...t 2 cables are installed at delivery To connect the UniNet 2 cable 1 Remove the termination plug from the last component in the UniNet 2 chain in the system 2 Connect the supplied UniNet 2 cable betwe...

Page 11: ...le union to the pump inlet 2 Connect the pump inlet tubing ETFE i d 1 0 mm to the union using a 1 16 finger tight connector Note In KTAexplorer systems route this tubing through the Connection part to...

Page 12: ...tor to the sample pump Filling a sample loop use a 22 cm long tubing with suitable inner diameter between the pump outlet and the flow restrictor IN port Route the tubing through the hole in the door...

Page 13: ...also find this information in KTAdesign Optional Configurations User Manual 2 9 Connecting an air sensor to Pump P 960 optional Air sensor Air 912N standard Air 912 Air 925 or Air 925N can be used at...

Page 14: ...ting the pump 1 Make sure that all liquid used in the pump is filtered 2 Place the liquid vessels on the workbench or on top of the system then use the flow restrictor see section 2 6 Connecting the f...

Page 15: ...low rate Note We recommend decreasing the flow rate of the system pump during equilibration before sample application and before switching the sample pump in line The sample pump will not start if the...

Page 16: ...at the factory If pressure pulsation or flow inaccuracy occur first remove any trapped air from the sample flow path as described in section 5 2 Removing trapped air bubbles from the sample pump Alte...

Page 17: ...tion 5 2 2 Removing air using a purge tubing 3 7 Using samples with high viscosity When using samples with high viscosity 4 5 cP at flow rates above 25 ml min it is necessary to use teflon tubings wit...

Page 18: ...ne liquid to another it is important that the two liquids are totally miscible with one another If the two are immiscible the pump should be flushed first with an intermediate liquid which is miscible...

Page 19: ...might loosen causing a jet of liquid to spray out This is especially dangerous if using hazardous chemicals Connect the tubing by first inserting the tubing fully then tightening the connector fingert...

Page 20: ...g prevents formation of air bubbles which can cause pressure pulsation and inaccurate flow rate 4 2 2 Additional recommendations for aqueous liquids After a run with an aqueous liquid the pump should...

Page 21: ...he pump from the system 5 Unscrew the two attachment screws to remove the connection part Use the 4 mm hex wrench 4 4 3 Installing the connection part 1 Wipe the rear side of the connection part and t...

Page 22: ...aning and replacing the check valves Faulty operation of the check valves is usually indicated by irregular flow very low flow or unstable pressure traces Probable causes are air dirt or a damage in a...

Page 23: ...valves using the screwdriver 4 6 3 Cleaning the check valves 1 Immerse the check valves in 100 ethanol and place in an ultasonic bath for 5 10 minutes 2 Repeat the ultrasonic bath with distilled water...

Page 24: ...pcs Outlet check valve short 2 pcs Outlet check valve long 1 pc The inlet check valves have line shaped holes The outlet check valves have round holes Fig 4 1 Check valves in Pump P 960 To install a...

Page 25: ...ertight CAUTION Only spare parts approved or supplied by Amersham Biosciences may be used for maintaining and servicing the pump Error symptom Possible cause Corrective action Leakage A tubing connect...

Page 26: ...flow rate is not calibrated properly Contact Amersham Biosciences for advice Erratic flow or pressure pulsation Leaking tubing connector Check the tubing connectors Tighten or replace if necessary Th...

Page 27: ...flow rate is too high Decrease the flow rate The pressure limit is too low Increase the pressure limit Not running No power to the system Check that the power to the system is switched on No UniNet 2...

Page 28: ...nually If there are only small amounts of air in the sample flow path remove the air as follows 1 Immerse the end of the sample inlet tubing in a suitable buffer 2 If using sample valve V5 set the val...

Page 29: ...the chosen sample inlet ports 1 7 Note The sample inlet tubings should be filled before filling the buffer tubing connected to port 8 4 Disconnect the connector fitted to the port that will be used fo...

Page 30: ...ow path tubing with buffer to remove any trapped air bubbles according to the procedure in section 5 2 1 Procedure for system not including sample valve V5 1 Put the sample inlet tubing in a buffer ve...

Page 31: ...is continuously monitored by the pressure sensor located above the connection part The signal which is proportional to the pump pressure is monitored by UNICORN via the UniNet 2 connection The connec...

Page 32: ...ng the connection part through the pump outlet the flow passes the pressure sensor where the pressure is measured The three plunger pumps are actuated by pistons in a sequential order meaning that the...

Page 33: ...ft 0 02 MPa 0 05 MPa Max 0 01 MPa week Tubing connectors pump inlet pump outlet pressure sensor inlet pressure sensor outlet male M6 male 1 16 finger tight male 1 16 finger tight male 1 16 finger tigh...

Page 34: ...re 100 Acetic acid 0 1 M Acetone 1 Aqueous buffers pH 2 12 Decon 90 10 for washing only Ethanol 20 Ethanol 96 for washing only Ethylene glycol 20 Glycerol 50 Guanidine 6 M HCl 0 1 M HCl 1 M for washin...

Page 35: ...check valve long 2 1 18 1172 44 Outlet check valve short 3 1 18 1172 45 Outlet check valve long 4 1 18 1172 46 O ring kit P 960 incl 1 42 1 52 mm for connection part 4 1 2 mm for inlet and outlet chec...

Page 36: ...m o d 1 8 and 3 16 tubing connector to P 960 inlet port 3 18 1127 76 Stop plug 5 16 PEEK 5 18 1112 50 Stop plug 1 16 PEEK 5 18 1112 52 PEEK tubing i d 0 75 mm o d 1 16 2 m 18 1112 53 Tefzel tubing i d...

Page 37: ...Reference information 6 Pump P 960 User Manual 18 1172 73 Edition AA 37 18117273aa book Page 37 Thursday July 3 2003 5 13 PM...

Page 38: ...6 Reference information 38 Pump P 960 User Manual 18 1172 73 Edition AA 18117273aa book Page 38 Thursday July 3 2003 5 13 PM...

Page 39: ...18117273aa book Page 39 Thursday July 3 2003 5 13 PM...

Page 40: ...at the unit is installed according to the installation instructions Pump P 960 is controlled from UNICORN For manual control use the instructions found in System Control Manual Pump 1 Switch on the pu...

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