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15
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3. Placing the Load
●
When using a waste processing bag or other kind of bag and sterilizing, place the bag in the wire
basket and insert it into the chamber. Using the bag “as is” can cause excessive temperature,
excessive pressure, boil-dry, etc.
●
Do not contact the mouth of the container which is placed in the chamber with the inner surface
of the lid. If the mouth of the container is closed by the lid inner surface, gas or liquid will gush out
of the container when opening the lid.
●
Be sure to use the bottom plate.
●
The mating surface of the chamber to the gasket is important for gas sealing.
Exercise care so as not to damage the section by hitting it with loaded objects.
●
Do not put in any object longer than the depth of the chamber stated in the specifications.
①
Put the objects to be sterilized
(dissolved material) in the chamber.
●
When sterilizing an empty deep container, lay the container on its side in the chamber so that it
will be permeated with steam. An upright position may cause insufficient sterilization.
●
If a waste disposal bag is used in sterilization, open the bag properly so that the bag is not in
contact with the inside surface of the chamber. Insufficient sterilization may be caused if the bag
is closed during sterilization. When the bag is opened excessively, steam is prevented from
circulating in the chamber. This may also result in insufficient sterilization.
●
Do not pile up the sterilization bucket in the chamber. When buckets are excessively piled, steam
fails to penetrate to all points, resulting in incomplete sterilization.
●
In sterilizing liquids such as medical solution and media, pay attention to the quantity of the liquid
in relation to its container. For an Erlenmeyer flask, the amount of liquid should be approx. 3/4 of
the capacity of the container; for a test tube, the appropriate quantity of chemical is approx. half
of the capacity of the container. Too much chemical may result in overflow from the container
during the temperature rising or cooling process.
●
Use container caps that are loose fitting and allow the passage of air. Containers may break if
venting is not possible.
●
In the case of dissolution of agar media, its quantity should be 2 liters or less per container.
Two liters or more of agar medium may not be completely dissolved.
●
Use the DURHAM TEST TUBE (Sample tube) with 6mm caliber or more. At the DURHAM TEST
TUBE (Sample tube) with less than 6 mm caliber, air bubble sometimes remains. Delaying of air
release time may allow no air bubbles to be left. Change the air release time by referring to
“6. Change of Function Setting-Air release time.”
CAUTION
IMPORTANT
△
NOTE
Summary of Contents for Hirayama HG-50
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