4
PCR Programs
Tips for Creating PCR Programs for the 384-well SureCycler 8800
42
Agilent SureCycler 8800 Setup and User’s Guide
Tips for Creating PCR Programs for the 384-well SureCycler 8800
When performing PCR on the 384- well block, reducing the duration of the
denaturation step during cycling may improve product yield for certain
enzymes. Denaturation times as short as 3–5 seconds may be optimal in
some protocols.
If you use the PCR Wizard to design a PCR, the program will need to be
edited after program creation to shorten the denaturation temperature
step. See
“To edit program step parameters”
on page 53 for instructions
on program editing.
Summary of Contents for SureCycler 8800
Page 1: ...Agilent Technologies SureCycler 8800 Setup and User s Guide Version A March 2011...
Page 4: ...4 Agilent SureCycler 8800 Setup and User s Guide...
Page 8: ...8 Agilent SureCycler 8800 Setup and User s Guide Contents...
Page 64: ...5 Maintenance and Updates Software Updates 64 Agilent SureCycler 8800 Setup and User s Guide...
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