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Agilent Technologies SPS 4, User Manual

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Agilent Technologies SPS 4 User Manual Download Page 71

71

Agilent Technologies

Agilent SPS 4 Autosampler
User’s Guide

7
Running the Calibration Wizard

Before User Calibration

72

Calibration Wizard Overview

72

Performing User Calibration

74

Error List

79

The sample probe can be displaced from the center after 
hitting a sample vial or the sides of the fixed wash reservoir. 
User calibration allows for minor adjustments to center the 
sample probe. This chapter provides instructions on 
performing user calibration for the Agilent SPS 4 
autosampler using the Calibration Wizard software.

If centering for the sample probe on your autosampler is 
misaligned, refer to 

Chapter 6

, "Troubleshooting".

Summary of Contents for SPS 4

Page 1: ...Agilent Technologies Agilent SPS 4 Autosampler User s Guide...

Page 2: ...s a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or disclo...

Page 3: ...ymbols 11 CE Compliance 12 Environmental Conditions 12 Electromagnetic Compatibility 13 2 Introduction Overview 16 Front and Rear Views 17 Front View 17 Rear View 19 Intended Use 21 Specifications 22...

Page 4: ...e Sample Probe 42 Fitting the Wash Pump Tubing 44 Two Drain Arrangements 46 Loading Wash Pump Tubing 47 Pressure Plate Adjustment 48 Connecting the Power and the Communications Cable 48 Confirming the...

Page 5: ...des 66 Initialization Problems 68 Probing Problems 69 Probe Arm Jam 69 When the Sample Probe Hits an Object 69 Communication Problems 70 7 Running the Calibration Wizard Before User Calibration 72 Cal...

Page 6: ...6 Agilent SPS 4 Autosampler User s Guide Contents Connections 92 Care and Maintenance 92 Preventing Earthquake Damage 94 Default Rinse Materials 95...

Page 7: ...that when used properly you have an accurate fast flexible and safe analytical system If the equipment is used in a manner not specified by Agilent the protection provided by the equipment may be imp...

Page 8: ...ther than that supplied with this equipment The power cord set that is shipped with this equipment cannot be used with other products Position the autosampler so that it is easy to access the mains po...

Page 9: ...ices for the safe handling of flammable liquids Other Precautions Use of the autosampler in any manner other than that specified by Agilent may impair the protection provided by the equipment Use of t...

Page 10: ...Off supply Hazardous voltage and potential for electrical shock Do not touch terminals that have this symbol when the equipment is on Caution refer to accompanying documentation Equipment marked with...

Page 11: ...et appareil ISM est conforme la norme NMB 001 du Canada This is the symbol for an Industrial Scientific and Medical Group 1 Class A product CISPR 11 South Korean Certification KC mark Warning Symbols...

Page 12: ...anies the product containing a copy of the Declaration of Conformity The Declaration of Conformity DoC is the legal declaration by Agilent that the product complies with the directives listed above an...

Page 13: ...tion is subject to the following two conditions 1 This equipment may not cause harmful interference 2 This equipment must accept any interference received including interference that may cause undesir...

Page 14: ...s Class A suitable for professional use and is for use in electromagnetic environments outside of the home A A ICES NMB 001 This ISM device complies with Canadian ICES 001 Cet appareil ISM est conform...

Page 15: ...15 Agilent Technologies Agilent SPS 4 Autosampler User s Guide 2 Introduction Overview 16 Front and Rear Views 17 Intended Use 21 Specifications 22 Options and Accessories 25...

Page 16: ...sories Depending on your application there is a choice of options Note that diameters in the following list mean outside diameters OD Standards Rack 34 well rack 12 wells for 29 mm diameter tubes 22 w...

Page 17: ...d up and down Z axis Figure 1 shows the front view of the Agilent SPS 4 Figure 1 Front view of the Agilent SPS 4 1 Spill Tray The spill tray sits on the bottom base bars of the Agilent SPS 4 and catch...

Page 18: ...pter The microtiter plate adapter is used to mount four 96 well microtiter plates on the rack location mat It occupies two sample rack positions 6 Fixed Wash Reservoir The fixed wash reservoir is loca...

Page 19: ...of Agilent SPS 4 1 Power Switch The power switch turns on off the Agilent SPS 4 I Power is ON O Power is OFF When the Agilent SPS 4 is turned on the initialization sequence runs to check the basic fu...

Page 20: ...Maximum two channels of three channel pump are used to pump the rinse solution to the fixed wash reservoir One channel can also be used to pump the used rinse solution to the waste container optional...

Page 21: ...es held in racks loaded on the rack location mat to the instrument for analysis The Agilent SPS 4 is controlled through software commands sent via a USB interface The Agilent SPS 4 should only be used...

Page 22: ...SPS 4 has an AC adapter It is rated at the following Table 1 Suitable environmental conditions for the Agilent SPS 4 Environmental Condition Specification Temperature Range Non Operating 20 C 60 C 20...

Page 23: ...d to be lifted by two people Table 3 Weights and dimensions Weights and Dimensions Specification Length 600 mm 23 6 in Depth 363 mm 14 3 in Height 510 mm 20 1 in Shipping Dimensions Packed in a Single...

Page 24: ...Indicators 4 Communications Connections Host USB 5 VDC 100 mA Max Auxiliary RS485 8 12 5 VDC 250 mA Max not used Host Interface Port USB Type A to Type B Auxiliary Port not used RS485 DB9 Male connec...

Page 25: ...le probe kit 1 2 mm ID Dual port fixed wash reservoir Microtiter plate adapter kit High uptake volume rinse kit Rinse tube kit for low volatile organic Pump drain kit Well plate startup kit Nebulizer...

Page 26: ...mm x 0 6 mm PTFE Tube 2 mm x 3 mm Tubing PVC 8 mm x 5 mm 2 m Tubing 2 5 mm ID 2 m Tubing solvent resistant 0 093 in ID 2 m Tubing solvent resistant 0 187 in ID 2 m Barb connector 2 5 mm 1 5 mm Fittin...

Page 27: ...position sample rack Allows 16 mm OD tubes to be used in 60 position rack Qty 2 recommended per rack Rack overlay for 40 position sample rack Allows 18 mm OD tubes to be used in 40 position rack Qty...

Page 28: ...28 Agilent SPS 4 Autosampler User s Guide 2 Introduction This page is intentionally left blank...

Page 29: ...Power and the Communications Cable 48 Confirming the Operation 50 Setting up the Instrument Controlling Software for SPS 4 50 Notes on Lifting the Autosampler 51 The Agilent SPS 4 autosampler is desi...

Page 30: ...ks 8 Fitting the Sample Probe 9 Fitting the Wash Pump Tubing 10 Connecting the Power and the Communications Cable 11 Confirming the Operation 12 Setting up the Instrument Controlling Software for SPS...

Page 31: ...Installation 3 Agilent SPS 4 Autosampler User s Guide 31 Figure 3 Autosampler orientation Rear Front Right Left X Axis Z Axis T Axis...

Page 32: ...length of capillary tubing connecting to the autosampler Inspection and Unpacking Unpacking and Lifting NOTE Keep the shipping box and packaging If you need to return the autosampler during the warra...

Page 33: ...heck all of the items you have received against the packing list A typical autosampler configuration could include the following items Spill tray Rack location mat Sample probe Standards rack Sample r...

Page 34: ...n a raised position to allow for easy access to individual samples or racks Fitting the Spill Tray NOTE When you install the autosampler with the optional Cover Kit install the Cover Kit before instal...

Page 35: ...ess of liquid into the spill tray is cleaned up immediately after a spill has occurred To access the spill tray for cleaning the sample racks the standards rack and the fixed wash reservoir together w...

Page 36: ...e rack location mat 1 Ensure that the spill tray is correctly fitted onto the autosampler base bars 2 One end of the rack location mat has a dovetail feature as shown in Figures 6 and 7 Orient the rac...

Page 37: ...gure 8 Single port fixed wash reservoir and kit The fixed wash reservoir is supplied as a kit with the inlet and outlet nipples fitted along with some lengths of inlet 2 5 mm ID tubing and outlet 5 0...

Page 38: ...ch in turn is connected to the outlet tubing from the wash pump For the dual port fixed wash reservoir there are two inlet nipples which will need to be connected to tubing from 2 separate channels of...

Page 39: ...ng on your application 34 well rack 12 wells for 29 mm OD tubes 22 wells for 17 mm OD tubes 5 well rack for 61 mm OD bottles To install the standards rack 1 Locate the holes for the standards rack in...

Page 40: ...side of the standards rack position The following rack options are supported 21 position rack for 30 mm OD tubes 24 position rack for 25 mm OD tubes 40 position rack for 20 mm OD tubes 60 position ra...

Page 41: ...k If a vial sits at an angle in the rack the probe may hit the side of the vial as it enters or leaves the vial This can affect the alignment of the probe and or damage the vial and the probe which mi...

Page 42: ...e probe and slide the nut off the sample probe 4 Place the mount nut from the sample probe on top of the accessories plate with the recessed edge facing up and the holes approximately aligned 5 Move t...

Page 43: ...e left around the probe arm housing When using a carbon fiber sample probe do not run the sample line through the hook or tube restraint at the top of the Z axis slide Connect the cable tie to the eye...

Page 44: ...ument s sample introduction system adjusting the length from the exit point as required Fitting the Wash Pump Tubing A wash pump is fitted on the rear of the autosampler and is used to supply fresh ri...

Page 45: ...u select a tube material that is compatible with the rinse solution If you are not sure which material to use contact your Agilent representative for a recommendation When a pump channel is not in use...

Page 46: ...h reservoir as shown in Figure 16 It then drains out from the outlet of the wash reservoir into the waste container by means of a gravity drain Figure 16 Gravity Drain Arrangement Pumped Drain Arrange...

Page 47: ...the right tube clamping block Ensure there are no twists or kinks in the tubing after loading as this will adversely affect tube life 4 Reposition the pressure plate over the rotor and fix by slotting...

Page 48: ...ker 3 Loosen the occlusion screw with a 5 5 mm 7 32 wrench until there is no pressure on the pump tubing 4 Turn the wash pump on using the instrument control software 5 While the wash pump is running...

Page 49: ...autosampler until all checks and settings for the operation have been completed Host Communications Port USB The host communications port USB is used to connect the autosampler to the computer contro...

Page 50: ...frame 3 The X axis may move to clear the end frame The probe arm rotates counter clockwise on the Theta axis and stops 4 The X axis homes against the left end frame 5 The probe arm moves to the four c...

Page 51: ...fixed wash reservoir and any associated tubing Remove the clear side covers if fitted It is advisable to lift the autosampler with two people positioning their hands through the end frames at either e...

Page 52: ...52 Agilent SPS 4 Autosampler User s Guide 3 Installation This page is intentionally left blank...

Page 53: ...Agilent Technologies Agilent SPS 4 Autosampler User s Guide 4 Operation Starting up the Autosampler 54 Shutting Down the Autosampler 55 Flushing the Wash Reservoir and Flow Path 55 Software Control 5...

Page 54: ...is illuminated green when the initialization sequence is completed correctly If an error occurs during the initialization sequence refer to Chapter 6 Troubleshooting 4 Adjust the pressure plates of t...

Page 55: ...Generally you can operate the autosampler without flushing the wash system wash reservoir and flow path Under normal circumstances you can simply drain the wash system prior to shutting down the autos...

Page 56: ...en flushed you can proceed with the sampling sequence or drain the wash system as part of the shutdown procedure Software Control All operations of the autosampler can be controlled by commands from t...

Page 57: ...aintenance Routine Maintenance 58 Cleaning 58 Replacing the Wash Pump Tubing 59 Lubrication 60 Service 60 Spare Parts 60 The Agilent SPS 4 autosampler has been designed for low maintenance and there a...

Page 58: ...r blockages Weekly Check the wash pump flow rates and replace the pump tubing if necessary Alternatively move the tubing so that the other set of bridges are secured in the peristaltic pump Cleaning T...

Page 59: ...g the autosampler 2 Release the pressure plate by disengaging the occlusion arm 3 Remove the old tubing and replace the pump tubing Carefully pull or cut the old tubing to remove it Replace the new tu...

Page 60: ...ampler is self lubricating and does not need the user to apply lubrication Service An authorized Agilent customer support representative must perform all servicing of the autosampler For contact infor...

Page 61: ...nt SPS 4 Autosampler User s Guide 61 Nipple PVDF 5 mm ID FWR Nipple PVDF 2 5 mm ID FWR Tubing PVC 8 mm OD x 5 mm ID Tubing 2 5 mm ID 2 meter length Barb connector 2 5 mm 1 5 mm Table 6 Consumable spar...

Page 62: ...62 Agilent SPS 4 Autosampler User s Guide 5 Maintenance This page is intentionally left blank...

Page 63: ...Probing Problems 69 Communication Problems 70 If your Agilent SPS 4 Autosampler is not operating correctly it can be difficult to determine whether the condition is caused by the autosampler the instr...

Page 64: ...ems on Page 65 If the power error indicator is not lit or the LEDs are lit RED refer to Operating State Problems on Page 65 For the error state indicated by the power error indicator refer to Error In...

Page 65: ...tosampler Turn the power off at the power point Check that the power cable is correctly seated into the power supply Check the power plug from the power supply is inserted correctly into the power sup...

Page 66: ...d error descriptions In the table E1 means the leftmost LED E4 means the rightmost LED 0 means LED off G means LED green on and R means LED red on If an error occurs a combination of the LEDs show the...

Page 67: ...Refer to Probing Problems on Page 69 for details R 0 0 0 8 Z axis position error The Z axis motor was commanded to move but did not reach the desired position Refer to Probing Problems on Page 69 for...

Page 68: ...or indicator illuminates to display the error condition See Table 7 for the error indicator codes Check the Agilent SPS 4 by doing the following 1 Turn the power off and move each axis manually until...

Page 69: ...attempt the initialization sequence again 3 If unsuccessful contact your Agilent customer support representative When the Sample Probe Hits an Object If the sample probe hits an object like a sample...

Page 70: ...upport representative Communication Problems 1 Check that the computer and the autosampler have the same communication settings 2 Disconnect the USB cable and initialize the autosampler to ensure that...

Page 71: ...can be displaced from the center after hitting a sample vial or the sides of the fixed wash reservoir User calibration allows for minor adjustments to center the sample probe This chapter provides ins...

Page 72: ...end frames The sample racks are correctly engaged in the locating holes in the rack location mat and are seated flat on the rack location mat A sample probe is fitted and has been mounted correctly Th...

Page 73: ...ust the X and Y axes positions of the sample probe at each calibration point Rear Left Rear Right Front Left and Front Right buttons Moves the probe to the calibration point Rear Left Rear Right Front...

Page 74: ...the drop down list and then click Connect in the Com Port field If you chose the wrong one an error message appears If the appropriate COM port number is not listed check the autosampler power the USB...

Page 75: ...a Adjust the sample probe height to the top of the sample well using the Up Down buttons b Adjust the XY position to the middle of the well using the arrow buttons c When the position of the probe is...

Page 76: ...n and the indicator next to the button labeled Rear Right changes to orange To adjust the probe position in point Rear Right a Adjust the sample probe height to the top of the sample well using the Up...

Page 77: ...on and the indicator next to the button labeled Front Left changes to orange To adjust the probe position in point Front Left a Adjust the sample probe height to the top of the sample well using the U...

Page 78: ...n and the indicator next to the button labeled Front Right changes to orange To adjust the probe position in point Front Right a Adjust the sample probe height to the top of the sample well using the...

Page 79: ...of the sample probe by clicking on each corner position button and driving the Z Axis position down Confirm that the sample probe is positioned in the center of each well Error List On running the ca...

Page 80: ...tion Wizard If the problem still remains unresolved contact your Agilent customer support representative Autosampler Error Example Move Z failed with ERR 8 An autosampler error occurs if the arm has a...

Page 81: ...81 Agilent Technologies Agilent SPS 4 Autosampler User s Guide A Appendices Installing the Cover Kit 82 Preventing Earthquake Damage 94 Default Rinse Materials 95...

Page 82: ...the samples from drafts dust and possible airborne contamination Protect the autosampler from corrosive vapors arising from handling acidic samples standards or rinse solution Figure 20 Autosampler C...

Page 83: ...oid fingerprints on the cover as a result of assembly Unpacking your Autosampler Cover Ensure you open the box from the top Remove each component in its individual wrapping and arrange these on a clea...

Page 84: ...cable ducting that clips onto the rear of the end frame to hold the power and communication cables in place 2 Remove the Spill Tray and the Rack Location Mat 3 Lay the unit forward onto its front whi...

Page 85: ...f the end frames with the fitted grommet to the right side of the wash pump tubing ducting as shown below 5 Lift the top of the end cover up over the edge of the chassis as shown below Grommet in pump...

Page 86: ...ting with the hole in the rear cover a small amount of force may be applied as shown below 7 Fasten the Rear Cover to the wash pump tubing ducting using the plastic screw and nut supplied as shown bel...

Page 87: ...communication cables through the cable ducting and clip onto the right end frame on the rear of the autosampler a Flare the bottom end of the cable ducting and fit to bottom of end frame cover slot as...

Page 88: ...s 9 Stand the autosampler back up onto its feet and re position The rear cover is now fitted as shown below 10 Re fit the Spill Tray 11 Re fit the Rack Location Mat carefully feeding the fixed wash re...

Page 89: ...net fitting clockwise as shown below 2 Take the side cover with the exhaust hose connection fitted and fit to the side of the autosampler that suits connection to an exhaust hose The side cover is pus...

Page 90: ...sition the front cover with the handle facing outwards and the bottom fold facing inwards Guide the front cover into the slots at the top of the end frames as shown below 2 Supporting the front cover...

Page 91: ...racks and standard racks tubes can be loaded or unloaded The door is held in the open position by a step in the end frame slots WARNING Dropping the front cover can cause liquid to splash from sample...

Page 92: ...on with a cable tie or hose clamp Care and Maintenance Do not use abrasive cleaners or strong solvents to clean the cover as this will reduce the clarity of the cover Use a soft cloth and household wi...

Page 93: ...have a cover kit installed if working with organic solvents Any failure in the extraction system could present a fire hazard CAUTION Failure to provide adequate extraction at all times will potentiall...

Page 94: ...endices Preventing Earthquake Damage If necessary prepare seismic brackets similar to those in figure below and secure the brackets NOTE Seismic brackets are not supplied by Agilent Prepare these item...

Page 95: ...water For your reference the standard supplied rinse lines contain the following materials Part Material Polyethylene tube 1 5 mm x 0 6 mm Polyethylene Peri pump tube Solva 3 bridged grey grey 12 pk P...

Page 96: ...96 Agilent SPS 4 Autosampler User s Guide A Appendices This page is intentionally left blank...

Page 97: ......

Page 98: ...2015 Printed in Malaysia Revision A May 2015 G8410 90000 G8410 90000 www agilent com Agilent Technologies In This Book This book explains the procedures necessary to use the Agilent SPS 4 Autosampler...

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