Agilent Technologies Cary 50 Hardware Manual Download

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Troubleshooting

Cary 50 Spectrophotometer Hardware Manual

Wavelength calibration

Problem

The position of an absorption (transmission or reflectance) peak
appears to shift over a prolonged period of time.

Solution



Perform a wavelength calibration.



Restart the computer. Agilent recommends that as part of your
standard operating procedure, you restart the computer attached
to the Cary 50 monthly.



Perform the Instrument Performance Test suite in ‘Validate’ to
ensure the instrument results meet specification.

Summary of Contents for Cary 50

Page 1: ...Cary 50 Spectrophotometer Hardware Manual ...

Page 2: ...ed in this document are furnished under a license and may be used or copied only in accordance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcontract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 ...

Page 3: ...s 6 Other precautions 7 Warning and caution messages 7 Information symbols 8 Color coding 9 CE compliance 10 2 Introduction 11 Installation requirements11 Documentation 11 Conventions 12 Specifications 12 Environmental 13 Weights and dimensions13 Computer power supply 13 Connections 14 3 Installation 15 Cell holder 16 Alignment 18 Other sample holders 18 ...

Page 4: ...rror 25 5 Troubleshooting 27 Windows XP service or driver failure 27 Access denied during installation28 FCary service failed to start 29 No green light indicating that the Cary 50 is powered 30 Start button replaced with Connect button 30 Hard disk power supply connection 32 Absorbance is 10 and fluctuates wildly during a scan 32 Instrument performance testing 33 Wavelength calibration 35 6 Spare...

Page 5: ...rly you have an accurate fast flexible and safe analytical system If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Information about safety practices appears throughout the documentation both hard copy and online provided with your instrument and accessories to help you safely operate the instrument and accessories Befo...

Page 6: ...The ONLY modules of the Cary 50 spectrophotometer that operators and other unauthorized personnel are permitted to access are the lamp module on the underside of the instrument and the sample compartment White circles indicate the screws you need to undo to remove the lamp module ALWAYS switch off the computer before changing a lamp The only panel you are permitted to remove is the snap out panel ...

Page 7: ...t in death serious personal injury and damage to equipment and property ALWAYS ensure that laboratory safety practices governing the use handling and disposal of such materials are strictly observed These safety practices should include the wearing of appropriate safety clothing and safety glasses Warning and caution messages Carefully read all warnings and cautions and observe them at all times A...

Page 8: ...ollowing triangular symbols appear in conjunction with warnings on the spectrometer and associated documentation The hazard they depict is shown below each symbol Broken glass Corrosive liquid Electrical shock Eye hazard Fire hazard Heavy weight danger to feet Heavy weight danger to hands Hot surface Noxious gas The following symbol may be used on warning labels attached to the instrument When you...

Page 9: ...uirements of one or more EU directives When attached to the rear of the product indicates that the product has been certified evaluated to CSA 1010 1 and UL 3101 1 Color coding The various indicator lights appearing on Agilent instruments and associated accessories are color coded to represent the status of the instrument or accessory The green indicator lamp on the front of the Cary 50 indicates ...

Page 10: ... that each product complies with the relevant directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with the directives is indicated by The CE marking appearing on the rear of the product The documentation package that accompanies the product containing a copy of the Declaration of Conformity This declaration is the legal declaration by A...

Page 11: ...he pReinstallation manual for future reference If you have misplaced your copy you can obtain a free replacement from your local Agilent office Alternatively download a PDF from the Agilent Web site www agilent com Documentation You have been provided with the following documentation to help you set up and operate your Agilent Cary 50 spectrophotometer Installation guides one for each supported op...

Page 12: ...ssories you ordered Conventions The following conventions have been used throughout the documentation Menus menu items buttons and check boxes have been typed in bold For example click OK and From the Edit menu choose Copy ALL CAPITALS indicate keyboard commands For example Press ENTER and Press SHIFT F3 NOTE Throughout this manual UV Dissolution and UV Fiber Optic Dissolution users should replace...

Page 13: ...5 50 95 8 80 NOTE For optimum analytical performance it is recommended that the ambient temperature of the laboratory be between 20 and 25 C and be held constant to within 2 C throughout the entire working day Weights and dimensions Weights Packed 23 kg 50 7 lb Unpacked 21 kg 46 lb Dimensions width x depth x height Packed 615 x 710 x 360 mm 24 x 28 x 14 in Unpacked 590 x 500 x 200 mm 23 x 20 x 8 i...

Page 14: ... driven accessories will increase the 12 V current by a further 2 A a maximum of 24 W for accessories Agilent recommends a computer power supply rating of 220 W as a minimum Connections 3 5 mm phono jack socket in the left side of the sample compartment for accessories 8 pin DIN connector in the left side of the sample compartment for the diode detector 25 pin D range connector in the right side o...

Page 15: ... Cary 50 weighs over 20 kg 44 lb To avoid injury to personnel or damage to equipment always use two or more people when lifting or carrying the instrument NEVER attempt to lift the instrument alone Details for unpacking the instrument and what to do in case it has been damaged in transit are outlined in the pReinstallation manual Following the instructions in the Cary 50 Installation Instructions ...

Page 16: ...the Maintenance chapter Cell holder A microcell holder see Figure 1 is supplied as standard with the Cary 50 Follow these instructions to install and align it Figure 1 Cell holder base left and microcell holder right 1 Locating holes 2 Cell holder base thumbscrew 3 Cell lifter 4 Microcell holder thumbscrew 5 Adjustment screw 6 Hexagonal ball driver To install the microcell holder 1 Place a microce...

Page 17: ...emove the sample compartment front panel refer to Figure 2 5 If not already fitted install the cell holder base in the sample compartment as follows a Place the cell holder base in the sample compartment aligning the two locating holes over the two locating pins in the floor of the sample compartment b Firmly tighten the thumbscrew on the cell holder base item 2 in Figure 1 6 Place the microcell h...

Page 18: ...l strike the cell aperture move the paper to the left of the cell and check that the beam is passing through the cell If the beam does not appear as though it will pass through the cell adjust the height of the cell as described on Page 17 7 Using the 2 5 millimeter hexagonal ball driver adjust the microcell holder adjustment screw item 5 in Figure 1 and note the intensity of the light striking th...

Page 19: ...ol Panel Add Remove Programs 2 Scroll through the list on the Install Uninstall tabbed page until you find Agilent Cary WinUV 3 Click Add Remove and then Yes Follow the instructions on the screen 4 Repeat Step 3 for the Cary WinUV Help Uninstalling the Cary 50 driver To uninstall the Cary 50 driver 1 Click the Windows Start button and choose Control Panel System 2 On the Hardware tab select Device...

Page 20: ...Installation 20 Cary 50 Spectrophotometer Hardware Manual This page is intentionally left blank ...

Page 21: ...gilent qualified or Agilent authorized customer support representatives WARNING Eye Hazard This instrument contains an intense light source Direct viewing of the light source will cause eye damage Operators and other unauthorized personnel must NEVER remove the main cover NOTE This section refers only to maintenance procedures for the instrument You should refer to your computer and printer plotte...

Page 22: ...he lamp module and realign the light beam Before changing the lamp module ALWAYS disconnect the Cary 50 from the computer NOTE These instructions are also provided in the Cary WinUV Help complete with a video demonstration Refer to the Cary WinUV Software Manual part number 8510162500 for details on using the online Help Replacing WARNING Shock and Eye Hazards When operating the lamp module emits ...

Page 23: ...3 Using a star head screwdriver undo and remove the six screws marked by the white solid circles on the lamp module cover refer to Figure 3 As you remove the last screw use one hand to support the lamp module Figure 3 Underneath the Cary 50 The six screws to be removed are numbered 4 Hold the lamp module out from the instrument and unplug the 3 pin connector from the Cary 50 refer to Figure 4 Disc...

Page 24: ...n connector of the new lamp module into the instrument 2 Fit the lamp module in the base of the Cary 50 ensuring that the wiring is kept clear of the other parts of the instrument 3 Replace and tighten the six screws 4 Return the instrument to its upright position and replace the cable at the rear 5 Reconnect the computer to the Cary 50 ...

Page 25: ...on and choosing All Programs Agilent Cary WinUV or Agilent UV UV FO Dissolution then Align 2 On the Cary tabbed page set the following parameters Beam Mode Single Beam Normal Y Mode T Ave Time 0 5000 Wavelength 500 nm 3 Click Apply 4 Remove the snap out panel refer to Figure 5 from the front of the instrument using a flat blade screwdriver to pry the panel open This exposes the two source mirror a...

Page 26: ...he screw in the other direction You can expect to see some fluctuation due to noise If the signal is out of range or excessively weak or strong click Rescale to bring the signal back into range for display Continue to adjust the screw until the length of the Current Signal bar is maximized When the length of the Current Signal bar is at its greatest repeat for the other adjustment screw NOTE It do...

Page 27: ...ains troubleshooting information to help you solve various problems you may encounter when setting up or using your Agilent Cary 50 hardware Windows XP service or driver failure Problem When you start the Microsoft Windows XP operation system you see a message Service Control Manager At least one service or driver failed during system startup Use Event Viewer to examine the event log for details S...

Page 28: ...een the Cary 50 driver and another driver on the system The most common cause is a sound card for which a driver has not been installed The computer may have a sound card built into the motherboard as well as a separate sound card that the computer recognizes but for which it does not have a driver installed You will need to 1 Click the Windows Start button and choose Control Panel System Click th...

Page 29: ...After installing the Cary WinUV software this message is displayed The FCary service failed to start due to the following error The system cannot find the file specified Solution This indicates the Cary 50 device driver was not copied to the correct directory It is possible you may not have access rights to the installation drive To rectify this 1 Log on as an administrator or ask your Windows XP ...

Page 30: ...tions for the connection of the power supply connector are listed in the installation guide supplied with your Cary 50 and in the Hard disk power supply connection section on Page 31 if you need to use a Y connector Start button replaced with Connect button Problem I have a Connect button instead of a Start button Solution Only one Cary WinUV at a time can communicate with the instrument If you wa...

Page 31: ...conflict with another card A defective Cary 50 PCI card To fix the problem when using Windows XP 1 Uninstall the Cary WinUV software see Page 28 2 Uninstall the Cary 50 driver see Page 19 3 Ensure the computer is turned off and the power cable has been removed Remove the Cary 50 PCI card from the computer 4 Restart the computer 5 Reinstall the Cary WinUV software and Cary 50 PCI according to the C...

Page 32: ...ary 50 fitted plug the appropriate end of the supplied Y connector refer Figure 6 into the connector at the end of the Cary 50 PCI card Figure 6 Y connector supplied with the Cary 50 2 Unplug a hard disk power supply connector and connect it to the Y connector 3 Connect the free end of the Y connector to the hard disk Absorbance is 10 and fluctuates wildly during a scan Problem The Cary 50 is repo...

Page 33: ...t side of the sample compartment is firmly plugged in Instrument performance testing Problem The results of your instrument performance tests do not meet specifications the results obtained during factory testing are included in the packing crate with the instrument Solution Check that The sample compartment is empty The cable connecting the instrument to the computer is correctly connected and th...

Page 34: ...ouble click the Cary WinUV folder or the UV Dissolution or UV FO Dissolution folder on the Windows desktop and then double click the System Information icon If the text reads UnCalibrated on the Calibration tabbed page select Initialize Wait for the instrument to finish initializing and click Calibrate When the calibration routine finishes the text will change to Calibrated Your instrument is now ...

Page 35: ...peak appears to shift over a prolonged period of time Solution Perform a wavelength calibration Restart the computer Agilent recommends that as part of your standard operating procedure you restart the computer attached to the Cary 50 monthly Perform the Instrument Performance Test suite in Validate to ensure the instrument results meet specification ...

Page 36: ...Troubleshooting 36 Cary 50 Spectrophotometer Hardware Manual This page is intentionally left blank ...

Page 37: ... indicated Part Part number Sample compartment lid 1 in Figure 8 0910127200 Sample compartment front panel 2 in Figure 8 0110642990 Lamp mirror adjustment cover 3 in Figure 8 0910127400 Accessory cable port plate 4 in Figure 8 0910124000 Xenon lamp module 0110639690 Cary 50 PCI card 0210155790 Cary 50 computer interconnecting cable 0110637390 3M disposable grounding wrist strap 7910031300 Mouse ma...

Page 38: ...2 1 3 4 Figure 8 Cary 50 showing 1 Sample compartment lid 2 Sample compartment front panel 3 Lamp mirror adjustment cover 4 Accessory cable port plate at rear Ordering details for other Cary 50 accessories are available on the Agilent Web site www agilent com ...

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