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Blood FlowMeter

Owner’s Guide

Blood FlowMeter

Owner’s Guide

Laser Doppler Monitoring

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Summary of Contents for Blood FlowMeter

Page 1: ...i Blood FlowMeter Owner s Guide Blood FlowMeter Owner s Guide Laser Doppler Monitoring ...

Page 2: ... their respective owners Document Number 6377 Rev D Date of issue 06 20 Copyright ADInstruments NZ Limited 2020 All rights reserved PowerLab LabChart and ADInstruments are registered trademarks of ADInstruments NZ Limited Windows 8 Windows 7 Windows 10 Windows Vista and NET Framework are trademarks of Microsoft Corporation Apple the Apple logo MacOS and Macintosh are trademarks of Apple Computer I...

Page 3: ...rt LCC Digital 16 Signal Output Connectors Analog 16 Fan Outlet 16 DC Power Connector 16 Power Switch 16 2 Setting Up 17 Connecting the Blood FlowMeter 18 Switching on the Blood FlowMeter 19 Connecting the Laser Doppler Probe 19 Probe Application 20 Temperature Warning 20 Probe Calibration 21 Using the Blood FlowMeter with LabChart 22 LDF Output Calibration 22 BSC Output Calibration 22 Removing th...

Page 4: ...s 25 Cleaning 25 Disinfection 26 Sterilization 26 B Troubleshooting 28 C Technical Aspects 31 Laser Doppler Flowmetry 32 LDF Theory 32 How it Works 33 Probe Operation 34 What the Blood FlowMeter Measures 34 The Blood Perfusion Unit BPU 34 Zero BPU 35 Motion Artifact Noise 35 D Speciﬁcations 37 Doppler Flow Specifications 37 Power Supply 38 Physical Configuration 38 Index 39 ...

Page 5: ...diagnose treat or monitor a subject Furthermore no product is intended for the prevention curing or alleviation of disease injury or handicap Where a product meets IEC 60601 1 it is under the principle that it is a more rigorous standard than other standards that could be chosen it provides a high safety level for subjects and operators The choice to meet IEC 60601 1 is in no way to be interpreted...

Page 6: ...nnection to human subjects even when there is direct electrical connection to the heart Warning symbol The exclamation mark inside a triangle means that the supplied documentation must be consulted for operating cautionary or safety information before using the device Further information is available on request Bio Amp Safety Instructions The Bio Amp inputs displaying any of the safety symbols are...

Page 7: ...mulus electrode The Isolated Stimulator output must not be used with individual physically separate stimulating electrodes Stimulation must not be applied across the chest or head Do not hold one electrode in each hand Always use a suitable electrode cream or gel and proper skin preparation to ensure a low impedance electrode contact Using electrodes without electrode cream can result in burns to ...

Page 8: ...tive General Safety Instructions To achieve the optimal degree of subject and operator safety consideration should be given to the following guidelines when setting up a PowerLab system either as stand alone equipment or when using PowerLab equipment in conjunction with other equipment Failure to do so may compromise the inherent safety measures designed into PowerLab equipment The following guide...

Page 9: ... manufacturer s cleaning instructions are not followed The ambient environment such as the temperature and relative humidity of the system should be kept within the manufacturer s specified range or the isolation barrier may be compromised The entry of liquids into equipment may also compromise the isolation barrier If spillage occurs the manufacturer of the affected equipment should be contacted ...

Page 10: ... not require periodic calibration or adjustment to ensure safe operation Internal diagnostic software performs system checks during power up and will report errors if a significant problem is found There is no need to open the instrument for inspection or maintenance and doing so within the warranty period will void the warranty Your PowerLab system can be periodically checked for basic safety by ...

Page 11: ...emperature in the range 5 35 C Non condensing humidity in the range 0 90 Disposal Forward to recycling center or return to manufacturer Unwanted equipment bearing the Waste Electrical and Electronic Equipment WEEE Directive symbol requires separate waste collection For a product labeled with this symbol either forward to a recycling center or contact your nearest ADInstruments representative for m...

Page 12: ...12 Blood FlowMeter Owner s Guide ...

Page 13: ...beds of skin and other tissues This Blood FlowMeter is LabChart compatible LCC which means it can be used directly with LabChart acquisition and analysis software in addition to an analog recording via a PowerLab data acquisition unit This owner s guide covers the features of the Blood FlowMeter its operation maintainence and safety information 1 C H A P T E R 1 1 Introduction ...

Page 14: ...ck that there are no obvious signs of damage to the outside of the Blood FlowMeter casing 2 Check that there is no obvious sign of internal damage such as rattling Pick up the Blood FlowMeter tilt it gently from side to side and listen for anything that appears to be loose If you have found a problem contact your authorized ADInstruments representative immediately and describe the problem so arran...

Page 15: ...h optical interfaces for the fiber optics in the probe cable The probes are designed to push and click into the connector Do not touch or tamper with any part of the connector as it may damage the optics or internal circuitry The Back Panel Probe Calibration Button The Blood FlowMeter is designed to automatically configure and calibrate itself for specific probes This operation requires a MLA191 C...

Page 16: ...larly PowerLabs The signal from the Output BSC represents the percentage backscatter tissue remittance It has a range of 0 5 V 1 remittance is 50 mV The Output LDF provides the laser Doppler flowmetry output signal in the range 0 5 V This signal represents the relative Blood Perfusion with 1 perfusion unit equalling 1 mV Fan Outlet In order to maintain the internal laser diode temperature the Bloo...

Page 17: ...DInstruments LabChart software You should first install the appropriate LabChart version for Windows or Macintosh on your computer Place the LabChart CD in the CD drive of your computer The installer should auto run If it doesn t see the Read Me on the CD Follow the onscreen instructions Next install the Blood FlowMeter Device Enabler The Blood FlowMeter Device Enabler is a software add on for Lab...

Page 18: ...ood FlowMeter to your PC or laptop using the USB cable supplied with the device Note Windows may display a message to indicate that the device driver is being installed when the Blood FlowMeter is turned on BNC Connection Analog Connect the BNC outputs of the Blood FlowMeter to the signal inputs of your recording device Note that the Blood FlowMeter has outputs for both LDF and BSC signals see Sig...

Page 19: ...onnect the probe to the probe connector on the front of the Blood FlowMeter orientate the plug with respect to the socket until it is aligned correctly The socket is designed to only accept the plug in one particular orientation Once aligned carefully push the plug into the socket until locked in position If everything is OK then the status indicator for the probe will glow amber If there is somet...

Page 20: ...at the probe site can disturb the blood perfusion reading Avoid direct illumination of the measurement site especially direct sunlight If erroneous readings of the measurement site from external lighting levels are suspected cover the attached probe and measurement area with a light piece of opaque material Probe signal outputs for different conditions are listed in Table 2 1 Condition LDF Backsca...

Page 21: ...y time by removing the probe or by pressing the CAL button during the calibration 1 Ensure the probe has been properly initialized for at least 10 minutes prior to attempting the calibration see Connecting the Laser Doppler Probe p 18 2 Using the positioning clamps provided with the MLA191 Calibration Kit immerse the probe to mid depth in the calibration solution 3 Press the CAL button once A sing...

Page 22: ...ge output per calibrated unit The LDF output produces 1 mV for each blood perfusion unit As the maximum output of the Blood FlowMeter is 5000 perfusion units or 5 V you should set the range of the corresponding input in LabChart to 5 V or more sensitively depending on your requirements As LDF output only produces an output in the range 0 5 V you can also set the Amplitude axis scale to single side...

Page 23: ...values required As the BSC output represents the relative strength of the returned signal it is a voltage that represents the percentage of backscattered light A 100 backscattered signal is represented by a voltage of 5 V Zero backscattering corresponds to 0 V This gives an output that corresponds to 50 mV per backscattering Removing the Probes To remove a probe from the instrument gently pull the...

Page 24: ...e outside cover of the Blood FlowMeter or to remove spills wipe the device with a soft lint free cloth Do not use alcohol or abrasive based cleaners as this may damage the external surfaces of the device Care of the Probes Handling the Probes Caution Probes for the Blood FlowMeter must be handled with care Failure to do this may result in breakage of the internal optical fibers scratching the poli...

Page 25: ...es be wiped with a soft cloth preferably one that does not shed fibers impregnated with 70 alcohol in water It is recommended that after use probes are cleaned immediately as it is easier to remove soiling and particulate matter before it dries onto the probe surfaces 1 Visually inspect the probe end cable and connector If there is no visible soiling wipe the probe end and cable with a soft cloth ...

Page 26: ...e end and cable in 2 glutaraldehyde or 70 alcohol in water The disinfectant manufacturer s recommended immersion times should be used Sterilization Only some of the dedicated perfusion probes may be sterilized by moist heat steam For further details please contact your ADInstruments representative ...

Page 27: ...27 Appendix A Care and Maintenance ...

Page 28: ...Lab hardware and software guides please contact your ADInstruments representative Although the Blood FlowMeter has been designed to be very reliable there may be occasions when it does not appear to function correctly In the majority of cases the problem can be fixed by checking connections and or resetting the Blood FlowMeter Very rarely will there be an actual problem with the Blood FlowMeter Th...

Page 29: ...t light when the probe is plugged in A faulty or damaged probe connector Check that all the connection pins are straight If one or more is bent do not attempt to bend them back Contact your ADInstruments representative for help The Blood FlowMeter internal processor is not working Turn off the power wait five seconds and turn on the power again If the Status indicator light still does not light th...

Page 30: ...rything else has been checked and there is still no laser output then you should contact you ADInstruments representative for service There is no signal output from the Blood FlowMeter No power to the Blood FlowMeter Check that the Power indicator on the Blood FlowMeter is on The probe is not plugged in correctly Check the probe is connected correctly A loose or missing output connection to your r...

Page 31: ...rtion of the bulk skin blood perfusion also fulfils the skin s metabolic requirements It is the upper dermis i e the first 1 5 4 0 mm of tissue depending on the site that is chiefly responsible for providing a nutritional supply of blood to the avascular epidermis the integrity of which is essential for the well being of the individual The degree of blood cell perfusion in this region of the micro...

Page 32: ...s scattered by moving blood cells are spectrally broadened according to the Doppler effect Maximum Doppler shifts occur when blood cells are moving in a direction parallel to the incident light beam and the detected light scattered light from the cells is detected in a direction opposite to its origin For example a Doppler shift of about 4 kHz is obtained when laser light of a wavelength of 830 nm...

Page 33: ...eturned signal which is then turned into a continuous signal that represents the blood perfusion in the illuminated area The block diagram below shows a very basic outline of the fundamental components The input channel is supplied with laser light at a wavelength of 830 nm visible red light from an internal semiconductor laser diode which is temperature controlled by means of a Peltier cell and f...

Page 34: ...to the blood perfusion in the area of measurement Probe Operation There are several types of optic probes that can be used with the system each one having its own optical characteristics In order to make sure that different probes will produce consistent results each probe contains a means of identifying itself to the system In this way you can change probes without having to recalibrate your syst...

Page 35: ...absolute zero is rarely obtained Even during total occlusion of the tissue blood perfusion there is often some small residual motion of blood cells trapped in the vessels as well as some small muscle and tissue movement in the measuring volume Even after surgical removal of tissue localized cell movement and Brownian motion may still occur in the severed blood vessels The Blood FlowMeter s interna...

Page 36: ...ilator or other mechanical device is employed Under these conditions artifacts should be minimized by controlling vibration reaching the measurement site and the Blood FlowMeter This may be achieved by placing the ventilator on a separate table and on a vibration absorbing medium ...

Page 37: ...ative units 0 100 corresponding to 0 5 V output Laser type Semiconductor laser diode temperature stabilized Laser wavelength 830 nm 10 nm Laser power 0 5 mW from probe Laser class Class 1 as per 21 CFR 1040 10 and 1040 11 Doppler signal bandwidth 16 kHz Linearity range Up to 0 35 moving scatterers by volume Flow response time 0 2 s Reading stability 5 measured with standard motility solution Zeroi...

Page 38: ...rnal enclosure Voltage range 100 240 V AC 50 60 Hz 0 8 0 4 A Output power 5 V 12 V DC Fusing Internal 2 A 240 V resettable Physical Conﬁguration Dimensions h w d 70 mm 240 mm 260 mm 2 70 9 45 10 2 Weight 2 5 kg Power requirements 240 V or 120 V internally set 15 W Operating temperature 5 35 C Operating humidity 0 70 non condensing ADInstruments reserves the right to alter these specifications at a...

Page 39: ...obes 18 connectors back panel 15 front panel 14 power 16 probe input 15 signal output 15 USB 16 cooling requirements 16 D Doppler effect 32 F fan outlet 16 front panel 14 I indicator lights power 15 probe status 15 L Laser Doppler Flowmetry 32 laser illumination 32 LDF output 32 LDF theory 32 M maintenance 10 microvascular perfusion 32 motion artifacts 35 O optic fiber 33 P power indicator light 1...

Page 40: ...4 red blood cell perfusion 34 removing probes 23 S Safety Notes 5 11 signal connection 18 signal outputs 15 sterilization of probes 26 storage 11 storage of probes 25 T technical aspects 31 troubleshooting 28 U using this guide 14 Z zero BPU readings 35 ...

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