Owner’s Guide
44
Setting up to Record From a Subject
This section provides a step-by-step guide to making a recording from a subject. Aft er
making the subject comfortable, a suitably qualified operator can use the following
steps to attach the headstage. The operator should follow their institute’s guidelines for
making neurological recordings and use precautions to minimize the risk of infection:
1.
Attach the headstage to the Neuro Amp EX.
2.
Securely attach the headstage to the subject by taping or bandaging it to the
limb or trunk.
3.
Insert all the pins into the headstage. Attach the Ground/reference electrode
first, using Figure 9–7 as a guide.
4.
Connect the active microelectrode (+) and the reference electrode (-) to the
positive and negative inputs of the headstage, respectively.
5.
The Audio Output from the Neuro Amp EX can be connected to external
speakers, if required.
6.
To help locate the nerve site, nerve stimulating techniques compatible with
your institute’s guidelines may be used. Stimulation may be made using the
ADInstruments Stimulus Isolator, or another Isolated Stimulator.
7.
Changes in signal output in response to nerve stimulation can be observed using
the Neuro Amp EX dialog or by recording data using Scope View in LabChart.
8.
If no longer required, move stimulation leads away from the headstage before
recording. Leaving them attached or close to the headstage will introduce noise.
For microneurography studies, the subject is normally passive during the procedure. The
headstage
must
be connected to the Neuro Amp EX before and during the procedure.
During the procedure, an operator:
•
securely attaches the headstage to the subject using tape or a bandage (Figure
9–9);
•
chooses a zero/reference point on the subject by attaching the Ground/Reference
electrode to the subject (Figure 9–10);
•
and, finally, connects the active and reference microelectrodes to the subject
(Figure 9–11).
The headstage must be securely attached (Figure 9–9) to the limb of the subject under
investigation. This ensures that the headstage is in contact with the subject and helps to
minimize artifacts from movement.
Summary of Contents for BIO
Page 1: ...Owner s Guide BIO AND NEURO AMPLIFIERS ...
Page 18: ...Owner s Guide 16 ...
