Accu-Scope 3019 Manual Download

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3019 FLUORESCENCE MICROSCOPE SYSTEM

ACCU-SCOPE

73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com

CONTENTS

SAFETY NOTES .................................................................................................................. 3

CARE AND MAINTENANCE ................................................................................................ 3

INTRODUCTION .................................................................................................................. 4

UNPACKING AND COMPONENTS DIAGRAMS .............................................................. 4-6

ASSEMBLY DIAGRAM & PROCEDURE ........................................................................ 7-10

ADJUSMENT & OPERATION

INTERPUPILLARY DISTANCE

......................................................................................... 11

FOCUSING

..................................................................................................................... 11

FOCUSING TENSION

..................................................................................................... 11

FIELD DIAPHRAGM

........................................................................................................ 12

APERTURE DIAPHRAGM

................................................................................................ 12

OIL OBJECTIVE

.............................................................................................................. 13

FLUORESCENCE OPERATION

PREPARATION

............................................................................................................... 14

ILLUMINATION

............................................................................................................... 15

FILTER FLASHBOARD

.................................................................................................... 15

CENTERING THE MERCURY LAMP

................................................................................ 16

CENTERING THE FIELD IRIS DIAPHRAGM

..................................................................... 17

SELECTING THE FLUORESCENCE FILTER CUBE

.......................................................... 17

TROUBLESHOOTING ........................................................................................................ 18

MAINTENANCE.................................................................................................................. 19

SERVICE ............................................................................................................................ 19

WARRANTY ....................................................................................................................... 19

Summary of Contents for 3019

Page 1: ...MANUAL 73 Mall Drive Commack NY 11725 631 864 1000 P 631 543 8900 F www accu scope com info accu scope com 3019 FLUORESCENCE MICROSCOPE SYSTEM ...

Page 2: ...6 ASSEMBLY DIAGRAM PROCEDURE 7 10 ADJUSMENT OPERATION INTERPUPILLARY DISTANCE 11 FOCUSING 11 FOCUSING TENSION 11 FIELD DIAPHRAGM 12 APERTURE DIAPHRAGM 12 OIL OBJECTIVE 13 FLUORESCENCE OPERATION PREPARATION 14 ILLUMINATION 15 FILTER FLASHBOARD 15 CENTERING THE MERCURY LAMP 16 CENTERING THE FIELD IRIS DIAPHRAGM 17 SELECTING THE FLUORESCENCE FILTER CUBE 17 TROUBLESHOOTING 18 MAINTENANCE 19 SERVICE 19...

Page 3: ...an 15 If the bulb is installed at an angle greater than this there is an increased risk of the mercury bulb bursting CARE AND MAINTENANCE 1 Do not attempt to disassemble any component including eyepieces objectives or focusing assembly 2 Keep the instrument clean remove dirt and debris regularly Accumulated dirt on metal surfaces should be cleaned with a damp cloth More persistent dirt should be r...

Page 4: ...es ensure each microscope is of the highest quality prior to shipment UNPACKING AND COMPONENTS Your microscope arrived packed in a molded Styrofoam container Do not discard the container the container should be retained for reshipment of your microscope if needed Avoid placing the microscope in dusty surroundings or in high temperature or humid areas as mold and mildew will form Carefully remove t...

Page 5: ... 73 Mall Drive Commack NY 11725 631 864 1000 www accu scope com 5 COMPONENTS DIAGRAM Lock Screw Y Axis Adjustment Knob Coarse Focusing Knob Fine Focusing Knob X axis Adjustment Knob Power Switch Iris Diaphragm Adjustment Control Objective Nosepiece ...

Page 6: ...ORESCENCE COMPONENTS DIAGRAM Lock Screw Condenser Adjustment Knob Mercury Bulb Adjustment Screws Mercury Lamp Housing Shutter Slider Field Diaphragm Adjustment Screw Mercury Lamp Power Supply Microscope Frame Fluorescence Filter Selection Lever Fluorescence Illuminator Eyepiece Head Field Diaphragm Adjustment Lever ...

Page 7: ...ans at our New York facility prior to shipment Should you need to disassemble assemble your microscope in the future please follow the instructions outlined below When assembling the microscope make sure that all parts are free of dust and dirt and avoid scratching any parts or touching glass surfaces Eyepiece Head Mercury Lamp House Mercury Lamp Socket Assembly Fluorescence Illuminator Mercury La...

Page 8: ... cubes can be installed in any order according to user preference You can install up to four 4 filter cubes two 2 on the right side and two 2 on the left side of the fluorescence illuminator When you are finished installing two blocks on either the right or left side slide the filter track back in replace the cover and tighten the screw IMPORTANT if the lock screw of a filter cube is not tightened...

Page 9: ...unt the positive pole the wide head before the negative pole or damage to the lamp may occur Never use excessive force when mounting the mercury lamp Avoid touching or soiling the mercury lamp If this happens gently wipe it with lens tissue or a soft clean cloth To prevent hazard always turn the main switch on the power supply unit to O OFF position unplug the power cord plug from the main outlet ...

Page 10: ...e mercury lamp house and tighten the lock screw Connect the other side of the plug to the port on the mercury lamp power supply Connect the one side of the power cord to the socket on the mercury power supply and the other to an electrical outlet that is well grounded The Fuse Fig 11 Make sure the main switch to the microscope and mercury power supply are in the off O position Remove the plug Fast...

Page 11: ...ng manner set both diopter collars to 0 Using your left eye only and the 10X objective focus your specimen by adjusting the coarse adjustment knob When the image is in view refine the image to its sharpest focus by turning the fine adjustment knob Rotate the diopter collar to obtain the sharpest focus To obtain the same sharp image using your right eye do not touch the coarse or fine adjustments I...

Page 12: ... the best performance and obtain the clearest image Fig 15 Adjusting the Aperture Diaphragm The aperture size is increased or decreased by rotating the condenser aperture diaphragm lever When the aperture is closed the brightness and resolution are decreased but the contrast and range of focus are increased If the aperture diaphragm is opened the brightness and resolution are increased however the...

Page 13: ...ctive back and forth Rotate the nosepiece so the 100xR oil immersion objective is in the light path With your eye at the level of the stage use the coarse focus knob to raise the stage with the specimen cover glass When you see a flash of light at this location the objective lens has made contact with the immersion oil and the microscope can now be focused using the fine focus knob Each time you f...

Page 14: ...ff of the mercury lamp will shorten its service life considerably Precautions on the specimen color fading The system employs high intensity excitation light to enable bright observation of dark fluorescence specimens As a result if high power objectives are used frequently color fading of the specimen occurs early degrading the view contrast of fluorescence images So it is effective to use the sh...

Page 15: ...s from the timer LIFE TIME press and hold the reset button RESET for more than 5 seconds The indicating range of the current CURRENT is 0 9 99A No need to switch on for transmitted illumination when using fluorescence illumination Do not cut off power supply within 15 minutes after mercury bulb is triggered to avoid damage to the bulb In order to prolong the life of mercury bulb please do not re l...

Page 16: ...g knob of mercury bulb and the horizontal adjusting knob of mercury bulb to make the bulb image on the scale of the slide Fig 22 A Adjust the knob of the parabolic mirror to focus the horizontal centering knob and the vertical centering knob to make the bulb mirror image on the scale of the slide Fig 22 B Adjust the knob and to make the bulb image and bulb mirror image symmetrical to the scale of ...

Page 17: ...ragm lever out until the diaphragm comes into the smallest state Use the two centering screws to adjust the two field iris diaphragm centering screws alternately to move the image of the diaphragm to the center Fig 24 shows the adjustment of diaphragm Move the field diaphragm lever to open the diaphragm As this makes slight deviation noticeable adjust the centering precisely Enlarge the diaphragm ...

Page 18: ...s not proper for the specimen Use proper mirror block The edge of the field of view is obscured or not evenly illuminated The objective is improperly engaged in the light path Make sure the nosepiece clicks properly into place The fluorescence mirror block is improperly engaged in the light path Engage it properly in the light path The field of view doesn t open fully Open it fully ND filter is st...

Page 19: ...a period of five years from the date of invoice to the original end user purchaser The mercury power supply is warranted for a period of one year from the date of invoice to the original end user purchaser This warranty does not cover damage caused in transit misuse neglect abuse or damage resulting from improper servicing or modification by other then ACCU SCOPE approved service personnel This wa...

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