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4titude 4s3, Operation Manual

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4titude 4s3 Operation Manual Download Page 7

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4s3™ Semi-Automatic Sheet Heat Sealer

4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0

1.2  Instrument Overview

1.2.1 Sealer Parts

Casing and Control Panel

Rear Power Interface

Heating Platen

Plate Carrier

1.2.2 Control Panel

Operate, Open Drawer &  

Incremental (up) Key

Three digit LED display

Set-up/Run Mode Key

Amber LED Status 

Indicator

Close Drawer &  

Decremental (down) Key

1.2.3 Rear Power Interface

Power Setting 115/230 Vac  

and Fuse Holder

I/O Power Switch 

Mains Power Plug 

Summary of Contents for 4s3

Page 1: ...s Park Damphurst Lane Wotton Surrey RH5 6QT T 44 0 1306 884885 F 44 0 1306 884886 E info 4ti co uk www 4ti co uk Semi Automatic Sheet Heat Sealer 4s3 Operation Manual Version 1 0 4s3 Semi Automatic Sheet Heat Sealer Operation Manual V1 0 en1 11 2018 ...

Page 2: ...his product complies with the following European standards 2014 35 EU Low Voltage Directive 2014 30 EU Electromagnetic Compatibility Directive 2011 65 EU Restriction of the use of certain hazardous Substances in electrical and electronic equipment RoHS Directive 2006 42 EC Machinery Directive Copyright Notice No part of this manual may be reproduced or transmitted in any form or by any means elect...

Page 3: ...rning Refer to manual The machine is designed for indoor laboratory use only at an altitude of less than 2200 m above sea level within a temperature range of 15 C to 35 C and a relative humidity range of 5 to 85 non condensing If the instrument is stored outside these ranges it should be left to stand until it equilibrates to within the above limits Ensure that the voltage selection switch is set ...

Page 4: ...rk proof Do not operate this product near volatile or flammable materials General Operation Safety When using infectious radioactive toxic and other solutions which may pose health risks please observe the appropriate safety precautions Do not use this machine in a potentially explosive environment or with potentially explosive chemicals Install the machine in a location free of excessive dust Avo...

Page 5: ...arning Control Settings 12 3 4 Operation Run Mode 13 3 5 Operation Stand by Mode 13 3 6 Power down Mode 14 3 7 Closing Drawer 14 4 Optimising Seal Quality 14 4 1 Plate Requirements 14 4 2 Plate Support Adapters 15 4 3 Heat Seal Materials 16 4 4 Sealing Parameters 16 5 Troubleshooting 18 5 1 Error Messages 18 5 2 Cleaning the Heating Platen 18 6 Maintenance 19 7 Appendix A Technical Specifications ...

Page 6: ... reliable sealing every time When using the 4s3 for the first time please read this entire operation manual carefully To guarantee problem free and safe operation it is essential to observe the following information 1 1 Unpacking Packing Contents Listing The device is delivered in an external carton and an internal carton with protective PE foam cushions Remove the 4s3 from each carton All packagi...

Page 7: ... Casing and Control Panel Rear Power Interface Heating Platen Plate Carrier 1 2 2 Control Panel Operate Open Drawer Incremental up Key Three digit LED display Set up Run Mode Key Amber LED Status Indicator Close Drawer Decremental down Key 1 2 3 Rear Power Interface Power Setting 115 230 Vac and Fuse Holder I O Power Switch Mains Power Plug ...

Page 8: ...nit has to be placed on a level surface away from direct sunlight ensuring access to the power switch at the back of the unit Ensure that the vents on the casing are not obstructed The mains voltage selector switch should be checked to ensure that the voltage has been set to the correct value Connect the unit to the mains power supply and switch on the I O switch on the rear power interface If the...

Page 9: ...nd on the power setting and fuse holder Changing the Voltage Setting 1 Open fuse holder compartment with a screwdriver 2 With the screwdriver lever out the fuse holder Opening fuse holder compartment Levering out fuse holder 3 Replace the two fuses according to the table below Power Voltage EN 60127 115 V 3 15 A T 230 V 2 A T 4 Insert the fuse holder keeping the set voltage label at the top 5 Clos...

Page 10: ...again to store the new sealing time value In doing so you will also exit from the programme menu The unit returns into run mode please refer to section 3 4 page 13 and the actual temperature of the heating platen is shown in the display 3 3 Stand by Power Down and Audible Warning Control Settings Press S for 10 seconds then release The display shows the symbol L flashing and the last set stand by ...

Page 11: ... use of the 4s3 Sealing Frame 4ti 0612 Press the OPERATE UP button with drawer open plate and adapter loaded The drawer will automatically close and the sealing process will begin Once the plate makes contact with the heating platen the display shows the symbol t and the countdown of the programmed sealing time starts When the countdown timer reaches zero the drawer automatically opens The sealed ...

Page 12: ...ng materials please reder to section 4 3 page 16 The 4s3 is designed to accommodate plates which meet the standard established by the Society of Biomolecular Sciences SBS These include but are not limited to deep well plates PCR and standard microplates in the 96 384 and 1536 well formats In accessing the suitability of a plate for heat sealing it is important to look at its design and the quality...

Page 13: ...ter PCR 96 4ti 0625 optional extra includes 4s3 Sealing Frame 4ti 0612 Note When using the PCR 96 adapter the standard plate adapter 4ti 0615 included should be removed from the drawer 4ti 0625 Fully skirted 1 component 384 well PCR plates FrameStar 384 well plates also compatible Plate Support Adapter PCR 384 4ti 0618 optional extra 4ti 0618 Shallow well plates e g FrameStar 384 Well PCR Plates P...

Page 14: ...heets 125 mm x 78 mm 100 4ti 05381 RA Random Access Pierce Heat Seal Strong sheets 127 mm x 100 mm 100 4ti 0536 Foil Heat Seal sheets 125 mm x 78 mm 100 4ti 0547 Polystyrene Foil Heat Seal sheets 127 mm x 100 mm 100 4ti 0591 Thermal Bond Heat Seal sheets 125 mm x 78 mm 100 4ti 0597 Gas Permeable Heat Seal Mark 2 sheets 125 mm x 78 mm 100 4ti 0597 ST Gas Permeable Heat Seal Mark 2 sheets sterile 12...

Page 15: ...eat Seal 170 180 2 3 160 170 2 4ti 0595 Gas Permeable Heat Seal Mark 2 165 175 3 165 175 3 175 185 3 4ti 0541 SLIT Gas Permeable Clear Heat Seal 175 185 2 3 165 180 3 185 200 3 4ti 0543 96 INDI Heat Seal 175 3 These values are for two component PCR plates For one piece polypropylene plates increase dwell time by one second These values can be used for all polystyrene plates 4 4 2 Sealing Parameter...

Page 16: ...ion ErC Drawer does not move or the system does not detect the movement ErD close drawer sensor and open drawer sensor error both the sensors are active at the same time The errors will remain displayed until the problem is solved To clear the error press the CLOSE DOWN button If any of the above errors persists switch OFF the unit and then switch ON again If within 10 seconds the problem is not s...

Page 17: ... automatically close The system will automatically heat back up to the last set temperature 6 Maintenance The unit does not require regular maintenance but it should be kept clean The external casing of the heat sealer can be cleaned with a cloth dipped in water or ethanol methanol formaldehyde can also be used The unit should not be immersed in solvents Do not use acetone or abrasive cleaners No ...

Page 18: ... humidity 20 80 not condensing Maximum altitude 2000 m Sound Pressure Level Maximum measured level 60 dbA below the limits of 85 dbA defined by regulation in force Safety Information Pollution degree 2 Overvoltage category II Devices for use indoors Setup Parameters Sealing temperature OFF or from 60 C to 200 C Step 1 C Default OFF Sealing time from 0 0 to 10 seconds Step 0 5 second Default 3 seco...

Page 19: ... plates 1 4ti 0614 4s3 Plate Support Adapter Random Access Standard Profile For use of Random Access Seals with standard profile plates 1 4ti 0614 1 4s3 Plate Support Adapter Random Access Standard Profile Break 2 Ways For use of Random Access Seals with standard profile FrameStar Break 2 Ways plates 1 4ti 0615 4s3 Plate Support Adapter Standard For skirted 96 and 384 well plates 1 4ti 0616 4s3 Pl...

Page 20: ...e problems with electrical power or usage not in accordance with product instructions if other than original spare parts supplied by the manufacturer have been used or if other than original 4titude seal sheets have been used The warranty does not automatically cover shipping charges Shipping costs both ways will be covered by 4titude where a returns number is issued within 8 weeks of the original...

Page 21: ...pping remove the plate carrier s from the unit Note If left inside the unit the plate carriers may cause severe damage to the sealer 2 Replace protective elastic bands 3 Push the CLOSE DOWN button to close the drawer DO NOT manually push the drawer closed 4 Switch off the unit Note if the unit is shipped without the protective elastic bands the front panel can be damaged during shipping 5 Place th...

Page 22: ...om the main power and then remove the power cord from the sealer 6 If present unplug the RS232 cable 11 2 Disposal Instruction INFORMATION FOR THE USERS In compliance with the Directives 2002 95 CE 2002 96 CE and 2003 108 CE concerning the restriction of the use of certain hazardous substances in the electrical and electronic equipment and the waste disposal the symbol of the crossed bin shown on ...

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